Post-Translational His-Cys Cross-Linkage Formation in Tyrosinase Induced by Copper(II)鈭扨eroxo Species

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• 作者：Nobutaka Fujieda ; Takuya Ikeda ; Michiaki Murata ; Sachiko Yanagisawa ; Shigetoshi Aono ; Kei Ohkubo ; Satoshi Nagao ; Takashi Ogura ; Shun Hirota ; Shunichi Fukuzumi ; Yukihiro Nakamura ; Yoji Hata ; Shinobu Itoh
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：February 9, 2011
• 年：2011
• 卷：133
• 期：5
• 页码：1180-1183
• 全文大小：743K
• 年卷期：v.133,no.5(February 9, 2011)
• ISSN：1520-5126

文摘

Autocatalytic formation of His-Cys cross-linkage in the enzyme active site of tyrosinase from Aspergillus oryzae has been demonstrated to proceed by the treatment of apoenzyme with Cu^II under aerobic conditions, where a (渭-畏²:畏²-peroxo)dicopper(II) species has been suggested to be involved as a key reactive intermediate.