Effect of Neural Stem Cells on Apoptosis of PC12 Cells Induced by Serum Deprivation

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• 作者：Xiangqin Li ; Tianqing Liu ; Kedong Song ; Shui Guan ; Leilei Zhu ; Dan Ge ; Zhanfeng Cui
• 刊名：Biotechnology Progress
• 出版年：2007
• 出版时间：August 2007
• 年：2007
• 卷：23
• 期：4
• 页码：952 - 957
• 全文大小：482K
• 年卷期：v.23,no.4(August 2007)
• ISSN：1520-6033

文摘

Neural stem cells (NSCs) have a bright application prospect to be used to treat neurodegenerativediseases due to their capacity to give rise to the appropriate cell types when they are grafted. Atpresent, however, the function of NSCs after transplantation is not quite ensured, whether toreplace the degenerative cells or to secrete nutrient factors. On the other hand, pheochromocytomacell line 12 (PC12) cells have been widely used for investigating Parkinson's disease (PD) sincetheir apoptosis is similar to that of dopaminergic neuron cells. Therefore, the possiblecytoprotective effects of NSCs on the apoptosis of PC12 cells induced by serum deprivationwere investigated in this paper. PC12 cells were cocultured with NSCs in DMEM/F12 mediumfree of serum, and their morphologies, viabilities, and survival were observed with an invertedmicroscope and assessed with a CCK-8 assay. In addition, the concentrations of glial derivedneurotrophic factor (GDNF) in different medium were detected with a GDNF Elisa kit, and themechanism of NSC's protective effect on PC12 cell apoptosis induced by serum deprivationwas analyzed. The results showed that (1) PC12 cell apoptosis induced by serum deprivationincreased with time, and only about 44.25% PC12 cells survived after 72 h; (2) NSCs culturemedium protected against PC12 cell apoptosis insignificantly; (3) NSCs' supernatant and NSCsmildly prevented PC12 cells from apoptosis; (4) the amount of GDNF secreted by NSCs increasedafter the coculture with the apoptotic PC12 cells induced by serum deprivation. It can be concludedthat there exists clear interaction between NSCs and apoptotic PC12 cells, and that GDNFsecretion from NSCs is one of the important mechanisms to prevent the apoptosis of PC12cells.