Development of a high-throughput multiplexed real time RT-PCR assay for detection of human pegivirus 1 and 2

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• 作者：Matthew Frankel ; ^{matthew.frankel@abbott.com} ; Kenn Forberg ; Kelly E. Coller ; Michael G. Berg ; John Hackett Jr. ; Gavin Cloherty ; George J. Dawson
• 关键词：RT-PCR ; PCR ; HPgV-2 ; Viral discovery ; Automation ; HCV ; Pegivirus
• 刊名：Journal of Virological Methods
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：241
• 期：Complete
• 页码：34-40
• 全文大小：896 K
• 卷排序：241

文摘

Development of multiplexed RT-PCR assay for simultaneous detection of HPgV-1 & 2. Identification of 8 additional samples positive for HPgV-2 RNA. Optimization described for the inclusion of 2 HPgV-2 targets and 1 HPgV-1. Assay provides opportunity to screen large cohorts using an automated platform.