Comparison of multiplex PCR hybridization-based and singleplex real-time PCR-based assays for detection of low prevalence pathogens in spiked samples

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• 作者：Donna Hockman^a ; Ming Dong^b ; Hong Zheng^b ; Sanjai Kumar^b ; Matthew D. Huff^a ; Elena Grigorenko^a ; Maureen Beanan^c ; Robert Duncan^b ; ^{robert.duncan@fda.hhs.gov}
• 关键词：Spiked specimens ; Blood-borne pathogens ; Real-time PCR ; Target Enriched Multiplex PCR
• 刊名：Journal of Microbiological Methods
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：132
• 期：Complete
• 页码：76-82
• 全文大小：351 K
• 卷排序：132

文摘

Standardized methods need to be established for evaluating low-prevalence pathogen diagnostic devices. Biothreat and emerging agents such as Francisella tularensis and Babesia microti are examples of low-prevalence pathogens. The effectiveness of standardized methods was demonstrated on two testing platforms, real-time PCR and TEM-PCR. Compared results from two types of sample matrix, healthy donor blood and blood from patients with signs of infection. Reproducibility of comparisons demonstrated the standardized methods are effective for evaluating diagnostic devices.