Purification and characterization of a fibrinolytic enzyme from the food-grade fungus, Neurospora sitophila

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• 作者：Xiao lan Liu ; Narasimha kumar Kopparapu ; Hong chen Zheng ; Priti Katrolia ; Yong ping Deng ; Xi qun Zheng ; ^{Zhengxiqun@126.com}
• 关键词：BSA ; bovine serum albumin ; SDS-PAGE ; sodium dodecyl sulfate polyacrylamide gel electrophoresis ; EDTA ; ethylenediamine tetracetic acid ; TPCK ; N-tosyl-l-phenylalanine chloromethyl ketone ; SBTI ; soybean trypsin inhibitor ; PMSF ; phenylmethane sulfonyl fluoride
• 刊名：Journal of Molecular Catalysis B: Enzymatic
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：134
• 期：part_PA
• 页码：98-104
• 全文大小：1148 K
• 卷排序：134

文摘

A novel fibrinolytic enzyme was purified from food-grade fungus, Neurospora sitophila. It is a monomer with molecular mass of 34 kDa; and has pI of 9.3 ± 0.2 as determined by IEF. It was optimally active at pH 7.6 and 41 °C; stable up to 52 °C and in wide pH 4–11. It hydrolyzed fibrin clot directly, and cleaved Aα, Bβ and γ-chains of fibrinogen. It can be developed as a safe potential candidate for oral fibrinolytic therapy.