Ct shift: A novel and accurate real-time PCR quantification model for direct comparison of different nucleic acid sequences and its application for transposon quantifications

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• 作者：Orsolya Kolacsek ; Enikő Pergel ; Nó ; ra Varga ; Á ; gota Apá ; ti ; Tamá ; s I. Orbá ; n ; ^{orban.tamas@ttk.mta.hu}
• 关键词：qPCR ; quantitative polymerase chain reaction ; Ct ; threshold cycle ; ΔΔCt ; comparative Ct ; RT-PCR ; reverse transcription PCR ; PGBD3 ; PiggyBac derived transposon family member 3 ; CS ; Cockayne syndrome ; CSB ; Cockayne syndrome B ; SB ; Sleeping Beauty ; Rcor1 ; REST corepressor 1 ; EGFP ; enhanced green fluorescent protein ; IRDR-L ; inverted repeat direct repeat &ndash ; left ; IRDR-R ; inverted repeat direct repeat &ndash ; right ; plst ; plasmid standard ; RPPH1 ; Ribonuclease P RNA component H1 ; Hsp90 ; heat shoc
• 刊名：Gene
• 出版年：2017
• 出版时间：20 January 2017
• 年：2017
• 卷：598
• 期：Complete
• 页码：43-49
• 全文大小：959 K
• 卷排序：598

文摘

The novel ‘Ct shift’ method is described for absolute real-time PCR quantification. The Ct shift is applied for exact determination of DNA transposon copy numbers. The method is used to determine the complex splicing pattern of the CSB-PGBD3 locus.