Digital RT-PCR method for hepatitis A virus and norovirus quantification in soft berries

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• 作者：Audrey Fraisse ; Coralie Coudray-Meunier ; Sandra Martin-Latil ; Catherine Hennechart-Collette ; Sabine Delannoy ; Patrick Fach ; Sylvie Perelle ; ^{sylvie.perelle@anses.fr}
• 关键词：Hepatitis A virus ; Norovirus ; Digital RT-PCR ; RT-qPCR ; Soft berries ; PCR inhibition
• 刊名：International Journal of Food Microbiology
• 出版年：2017
• 出版时间：21 February 2017
• 年：2017
• 卷：243
• 期：Complete
• 页码：36-45
• 全文大小：1241 K
• 卷排序：243

文摘

Quantification of HAV and noroviruses in red fruits Comparison of digital RT-PCR and RT-qPCR Significant reduction of PCR inhibition with additional purification step of RNA Significant reduction of PCR inhibition with RT-dPCR This study offers reliable alternative methods to quantify viruses in red fruits.