Molecular evolution of influenza A/H3N2 viruses in the province of Québec (Canada) during the 1997–2000 period

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• 作者：Hardy ; Isabelle ; Li ; Yan ; Coulthart ; Michael B. ; Goyette ; Nathalie ; Boivin ; Guy
• 关键词：Influenza ; Hemagglutinin ; Molecular characterization ; Drift
• 刊名：Virus Research
• 出版年：2001
• 出版时间：August, 2001
• 年：2001
• 卷：77
• 期：1
• 页码：89-96
• 全文大小：105 K

文摘

In this study, we compared antigenic (hemagglutination inhibition (HI) assay) and molecular (sequencing of the hemagglutinin (HA1) gene) characterization of influenza isolates collected in the Province of Québec (Canada) during the last three flu seasons (1997–2000). Twenty-three isolates were tested by a standard HI assay and 37 by sequencing of the HA1 gene for their homology to the A/H3N2 vaccine strains A/Wuhan/359/95 (1997–1998) and A/Sydney/5/97 (1998–1999 and 1999–2000). By HI, two isolates were antigenically similar to A/Wuhan/359/95 (both from 1997 to 1998), 16 were similar to A/Sydney/5/97 (1997–2000) and no conclusions could be inferred for the other five isolates due to identical HI titers for the two vaccine strains (n