Diffusion time (Δ) effect in diffusion measurements has been validated as a sensitive biomarker in liver fibrosis by rat models. To extend this finding to clinical study, a reliable imaging technique is highly desirable. This study aimed to develop an optimal stimulated echo acquisition mode (STEAM) diffusion-weighted imaging (DWI) method dedicated to human liver imaging on 3 Tesla (T) and preliminarily investigate the dependence effect in healthy volunteers.