The Tianluoshan Site in Yuyao City is a relatively complete Neolithic site about early Hemudu culture.  The authors analyzed 22 samples with an interval of 10cm in section TLS1 and 3 samples in section TLS2. Detailed evidence of phytolith，diatom，charcoal and sponge spicule from the Tianluoshan Site reveals a cultural and environmental context of ancient human activity. It came to the conclusions that rice cultivation began in the early of Hemudu culture and that there were two relatively high sea level periods before and after Tianluoshan cultural layers. These two relatively high sea level periods maybe be related to the development and disappearance of Hemudu Culture.