巢湖低温高效聚磷菌的筛选及其特性
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摘要
采用低温富集培养及混合平板分离技术,从巢湖底泥中分离筛选得到2株在低温下仍具有较高效能的菌株D3、D6,经过厌氧/好氧交替培养,2种菌株在低温下(8℃)的除磷率均达到80%以上。在低温下,研究了p H、微量元素对2株菌株生长及除磷率的影响,实验结果表明,2株菌都具有广泛的温度适应性,适宜其生长和除磷的p H为中性偏碱,微量元素的缺乏对2株菌株的生长和除磷效果有不同程度的不良影响。染色观察显示,厌氧培养时菌体内聚羟基丁酸(PHB)颗粒明显增多,转为好氧培养后异染颗粒增多,为典型的聚磷菌特征。经鉴定,2株菌均属假单胞菌属。
Using low-temperature enrichment culture and mixed plate separation technology,two strains( D3,D6) with preferred phosphorus accumulation at low temperature were isolated from the sediment in Chaohu Lake. After the alternating anaerobic / aerobic cultivation,the phosphorus removal rate of two strains at low temperature( 8℃) reached more than 80%. More important factors( p H,microelements) that affected two strains on growth and phosphorus removal rate were studied at low temperatures. The experimental results showed that two strains have wide temperature adaptability,and the optimal p H for the two strains of growth and phosphorus removal is neutral to slightly alkaline,the lack of microelements in two strains of growth and phosphorus removal effect at different levels. Staining observation showed that the polyphydroxybutyrate( PHB) particles increased obviously at the anaerobic stage,and metachromatic granules increased accordingly at the aerobic stage,which were the typical characteristics of the PAOs. Both of the isolated strains were identified as Pseudomonas sp.
Using low-temperature enrichment culture and mixed plate separation technology,two strains( D3,D6) with preferred phosphorus accumulation at low temperature were isolated from the sediment in Chaohu Lake. After the alternating anaerobic / aerobic cultivation,the phosphorus removal rate of two strains at low temperature( 8℃) reached more than 80%. More important factors( p H,microelements) that affected two strains on growth and phosphorus removal rate were studied at low temperatures. The experimental results showed that two strains have wide temperature adaptability,and the optimal p H for the two strains of growth and phosphorus removal is neutral to slightly alkaline,the lack of microelements in two strains of growth and phosphorus removal effect at different levels. Staining observation showed that the polyphydroxybutyrate( PHB) particles increased obviously at the anaerobic stage,and metachromatic granules increased accordingly at the aerobic stage,which were the typical characteristics of the PAOs. Both of the isolated strains were identified as Pseudomonas sp.
引文
[1]Morohoshi T.,Yamashita T.,Kato J.,et al.A method for screening remove polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater.Journal of Bioscience and Bioengineering,2003,95(6):637-640
[2]蔡天明,管莉菠,崔中利,等.高效聚磷菌株GM1的分离和聚磷特性研究.土壤学报,2005,42(4):635-641Cai Tianming,Guan Libo,Cui Zhongli,et al.Isolation and characterization of strain GM1 with high capability of accumulating Poly-P.Acta Pedologica Sinica,2005,42(4):635-641(in Chinese)
[3]Aslan S.,Kapdan I.K.Batch kinetics of nitrogen and phosphorus removal from synthetic wastewater by algae.Ecological Engineering,2006,28(1):64-70
[4]Hao Xiaodi,Van Loosdrecht M.C.M.,Meijer S.C.F.,et al.Model-based evaluation of two BNR processes-UCT and A2N.Water Research,2001,35(12):2851-2860
[5]王春丽,马放,王强.一株耐低温反硝化聚磷菌的筛选及其特性研究.环境工程学报,2007,1(4):21-24Wang Chunli,Ma Fang,Wang Qiang.Screening of a low temperature-resistant denitrifying polyphosphate-accumulating organisms and its characteristics.Chinese Journal of Environmental Engineering,2007,1(4):21-24(in Chinese)
[6]李智勇,王淑滢,吴凡松,等.强化生物除磷体系中反硝化聚磷菌的选择与富集.环境科学学报,2004,24(1):45-49Li Yongzhi,Wang Shuying,Wu Fansong,et al.Selection and enrichment of denitrifying phosphate accumulating bacteria in biologically enhanced phosphate removal process.Acta Scientiae Circumstantiae,2004,24(1):45-49(in Chinese)
[7]慕庆峰,文波龙,贝丽霞,等.一株反硝化聚磷菌的脱氮、除磷能力及其固定化净化水体的研究.湿地科学,2013,11(2):227-232Mu Qingfeng,Wen Bolong,Bei Lixia,et al.Nitrogen and phosphorus removal ability of a denitrifying phosphate accumulating bacteria and its immobilization on water purification.Wetland Science,2013,11(2):227-232(in Chinese)
[8]Stante L.,Cellamare C.M.,Malaspina F.,et al.Biological phosphorus removal by pure culture of Lamnropedia spp..Water Research,1997,31(6):1317-1324
[9]周群英,高廷耀.环境工程微生物(第2版).北京:高等教育出版社,2000
[10]刘艳杰,李建政,许修宏,等.高效低温聚磷菌的筛选及特性研究.环境科学与管理,2012,37(5):36-40Liu Yanjie,Li Jianzheng,Xu Xiuhong,et al.Screening of efficient and low-temperature polyphosphate-accumulating organisms and their characteristics.Environmental Science and Management,2012,37(5):36-40(in Chinese)
[11]李楠,王秀蘅,亢涵,等.p H对低温除磷微生物种群与聚磷菌代谢的影响.环境科学与技术,2013,36(3):9-11Li Nan,Wang Xiuheng,Kang Han,et al.Influence of p H on microbial community structure and metabolic characteristics of PAOs in biological phosphorus removal at low temperature.Environmental Science&Technology,2013,36(3):9-11(in Chinese)
[12]傅宏兵.高效聚磷菌的分离及其特性研究.合肥:安徽大学硕士学位论文,2009,Fu Hongbing.Study on isolation and characterization of phosphorus-accumulating bacteria.Hefei:Master Dissertation of Anhui University,2009(in Chinese)
[13]胡筱敏,杨辉.新型A/O/A/O-SBR中DPB的富集及其典型菌株特性研究.安全与环境学报,2012,12(4):104-108Hu Xiaomin,Yang Hui.On enrichment way and characteristic features of typical DPB strains in a new species of A/O/A/O-SBR.Journal of Safety and Environment,2012,12(4):104-108(in Chinese)
[14]南亚萍,袁林江,王洋.金黄色葡萄球菌聚磷特性研究.环境科学学报,2013,33(4):1028-1034Nan Yaping,Yuan Linjiang,Wang Yang.Characteristic and performance of phosphate accumulation by Staphyloccocus aureus.Acta Scientiae Circumstantiae,2013,33(4):1028-1034(in Chinese)
[2]蔡天明,管莉菠,崔中利,等.高效聚磷菌株GM1的分离和聚磷特性研究.土壤学报,2005,42(4):635-641Cai Tianming,Guan Libo,Cui Zhongli,et al.Isolation and characterization of strain GM1 with high capability of accumulating Poly-P.Acta Pedologica Sinica,2005,42(4):635-641(in Chinese)
[3]Aslan S.,Kapdan I.K.Batch kinetics of nitrogen and phosphorus removal from synthetic wastewater by algae.Ecological Engineering,2006,28(1):64-70
[4]Hao Xiaodi,Van Loosdrecht M.C.M.,Meijer S.C.F.,et al.Model-based evaluation of two BNR processes-UCT and A2N.Water Research,2001,35(12):2851-2860
[5]王春丽,马放,王强.一株耐低温反硝化聚磷菌的筛选及其特性研究.环境工程学报,2007,1(4):21-24Wang Chunli,Ma Fang,Wang Qiang.Screening of a low temperature-resistant denitrifying polyphosphate-accumulating organisms and its characteristics.Chinese Journal of Environmental Engineering,2007,1(4):21-24(in Chinese)
[6]李智勇,王淑滢,吴凡松,等.强化生物除磷体系中反硝化聚磷菌的选择与富集.环境科学学报,2004,24(1):45-49Li Yongzhi,Wang Shuying,Wu Fansong,et al.Selection and enrichment of denitrifying phosphate accumulating bacteria in biologically enhanced phosphate removal process.Acta Scientiae Circumstantiae,2004,24(1):45-49(in Chinese)
[7]慕庆峰,文波龙,贝丽霞,等.一株反硝化聚磷菌的脱氮、除磷能力及其固定化净化水体的研究.湿地科学,2013,11(2):227-232Mu Qingfeng,Wen Bolong,Bei Lixia,et al.Nitrogen and phosphorus removal ability of a denitrifying phosphate accumulating bacteria and its immobilization on water purification.Wetland Science,2013,11(2):227-232(in Chinese)
[8]Stante L.,Cellamare C.M.,Malaspina F.,et al.Biological phosphorus removal by pure culture of Lamnropedia spp..Water Research,1997,31(6):1317-1324
[9]周群英,高廷耀.环境工程微生物(第2版).北京:高等教育出版社,2000
[10]刘艳杰,李建政,许修宏,等.高效低温聚磷菌的筛选及特性研究.环境科学与管理,2012,37(5):36-40Liu Yanjie,Li Jianzheng,Xu Xiuhong,et al.Screening of efficient and low-temperature polyphosphate-accumulating organisms and their characteristics.Environmental Science and Management,2012,37(5):36-40(in Chinese)
[11]李楠,王秀蘅,亢涵,等.p H对低温除磷微生物种群与聚磷菌代谢的影响.环境科学与技术,2013,36(3):9-11Li Nan,Wang Xiuheng,Kang Han,et al.Influence of p H on microbial community structure and metabolic characteristics of PAOs in biological phosphorus removal at low temperature.Environmental Science&Technology,2013,36(3):9-11(in Chinese)
[12]傅宏兵.高效聚磷菌的分离及其特性研究.合肥:安徽大学硕士学位论文,2009,Fu Hongbing.Study on isolation and characterization of phosphorus-accumulating bacteria.Hefei:Master Dissertation of Anhui University,2009(in Chinese)
[13]胡筱敏,杨辉.新型A/O/A/O-SBR中DPB的富集及其典型菌株特性研究.安全与环境学报,2012,12(4):104-108Hu Xiaomin,Yang Hui.On enrichment way and characteristic features of typical DPB strains in a new species of A/O/A/O-SBR.Journal of Safety and Environment,2012,12(4):104-108(in Chinese)
[14]南亚萍,袁林江,王洋.金黄色葡萄球菌聚磷特性研究.环境科学学报,2013,33(4):1028-1034Nan Yaping,Yuan Linjiang,Wang Yang.Characteristic and performance of phosphate accumulation by Staphyloccocus aureus.Acta Scientiae Circumstantiae,2013,33(4):1028-1034(in Chinese)
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