菜豆再生体系及遗传转化体系研究进展
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摘要
菜豆分子育种及功能基因组学分析需要高效稳定的再生体系和遗传转化体系。归纳了近年来国内外菜豆再生体系及遗传转化体系的研究进展。首先阐述了菜豆再生方法及研究现状,其中包括基于器官发生和体细胞胚发生不同途径菜豆再生体系的建立和相关的影响因素。其次概括了农杆菌介导法在菜豆遗传转化方面的研究进展,并分析了影响农杆菌介导遗传转化效率的主要因素,包括受体外植体类型和外植体损伤、农杆菌菌株及菌液浓度、共培养条件(时间、温度、光照时间)、乙酰丁香酮浓度等。然后叙述了有关基因枪介导菜豆遗传转化的研究进展。最后对目前菜豆再生体系和遗传转化体系存在的难题进行了总结,同时对今后研究的方向和思路做出了比较可靠的趋向预测,以期为建立高效稳定的菜豆再生体系和遗传转化体系提供参考依据。
High-efficient and stable regeneration system and genetic transformation system are required for molecular breeding and functional genomics analysis of common bean. The regeneration system and Agrobacterium-mediated transformation of common beans at home and abroad in recent years were summarized. Firstly, the regeneration methods and research status of common bean were introduced, including the establishment of regeneration system based on organogenesis and somatic embryogenesis and related impact factors. Secondly, the research progress of Agrobacterium-mediated transformation in common bean was summarized, and the main factors affecting the efficiency of Agrobacterium-mediated transformation were analyzed, including the types of receptor explants and the damage of explants, Agrobacterium strain and bacterial concentration, co-culture conditions(time, temperature, the time of illumination), acetosyringone concentration, etc. Then the progress of gene gun-mediated transformation of common bean was reviewed.Finally, the problems in current regeneration system and genetic transformation system of common bean were summarized. At the same time, the future research directions and ideas were predicted, in order to provide references for the establishment of high efficient and stable regeneration system and genetic transformation system of common bean.
High-efficient and stable regeneration system and genetic transformation system are required for molecular breeding and functional genomics analysis of common bean. The regeneration system and Agrobacterium-mediated transformation of common beans at home and abroad in recent years were summarized. Firstly, the regeneration methods and research status of common bean were introduced, including the establishment of regeneration system based on organogenesis and somatic embryogenesis and related impact factors. Secondly, the research progress of Agrobacterium-mediated transformation in common bean was summarized, and the main factors affecting the efficiency of Agrobacterium-mediated transformation were analyzed, including the types of receptor explants and the damage of explants, Agrobacterium strain and bacterial concentration, co-culture conditions(time, temperature, the time of illumination), acetosyringone concentration, etc. Then the progress of gene gun-mediated transformation of common bean was reviewed.Finally, the problems in current regeneration system and genetic transformation system of common bean were summarized. At the same time, the future research directions and ideas were predicted, in order to provide references for the establishment of high efficient and stable regeneration system and genetic transformation system of common bean.
引文
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[3]张欣,付亚萍,周君莉,等.水稻规模化转基因技术体系构建与应用[J].中国农业科学,2014,47(21):4141-4154.
[4]刘允军,贾志伟,刘艳,等.玉米规模化转基因技术体系构建及其应用[J].中国农业科学,2014,47(21):4172-4182.
[5]叶兴国,徐惠君,杜丽璞,等.小麦规模化转基因技术体系构建及其应用[J].中国农业科学,2014,47(21):4155-4171.
[6] Malik K A, Saxena P K. Regeneration in Phaseolus vulgaris l.:High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron[J].Planta,1992,186(3):384-389.
[7] Delgado-sanchez P, Saucedo-ruiz M, Gonzalez-chavira M, et al. An organogenic plant regeneration system for common bean(Phaseolus vulgaris L.)[J].Plant Science,2006,170(4):822-827.
[8] Mukeshimana, Gerardine, Kelly, et al. Factors influencing regeneration and Agrobacterium tumefaciens-mediated;transformation of common bean(Phaseolus vulgaris L.)[J].Plant Biotechnology Reports,2013,7(1):59-70.
[9] Dang W, Wei Z M. High frequency plant regeneration from the cotyledonary node of common bean[J].Biologia Plantarum,2009,53(2):312-316.
[10] Ahmed E E, Bisztray G D, Velich I. Plant regeneration from seedling explants of common bean:Phaseolus vulgaris L[J].ACTA Biologica Szegediensis,2002,46(3/4):27-28.
[11] Franklin C I, Trieu T N, Gonzales R A, et al. Plant regeneration from seedling explants of green bean(Phaseolus vulgaris L)via organogenesis[J].Plant Cell Tissue&Organ Culture,1991,24(3):199-206.
[12]朱有光,奚惕.菜豆的组织培养和植株再生[J].植物生理学通讯,1988(2):60.
[13] Zambre M A, Clercq J D, VranováE, et al. Plant regeneration from embryo-derived callus in Phaseolus vulgaris L.(common bean)and P. acutifolius A. Gray(tepary bean)[J].Plant Cell Reports,1998,17(8):626-630.
[14] Kwapata K, Sabzikar R, Sticklen M B, et al. In vitro regeneration and morphogenesis studies in common bean[J].Plant Cell Tissue&Organ Culture,2010,100(1):97-105.
[15] Saxena M K. Regeneration in Phaseolus vulgaris l.:promotive role of N6-benzylaminopurine in cultures from juvenile leaves[J].Planta,1991,184(1):148-150.
[16] Carvalho M D, Le B V, Zuily-fodil Y, et al. Efficient whole plant regeneration of common bean(Phaseolus vulgaris L.)using thincell-layer culture and Silver nitrate[J].Plant Science,2000,159(2):223-232.
[17]董少鸣.愈伤组织培养实验中几种外植体的选择[J].河北民族师范学院学报,2007,27(2):62-62.
[18] Klu G P, Jacobsen E, Harten A V. Induced mutations in winged bean(Psophocarpus tetragonolobus L. DC)with low tannin content[J].Euphytica,1997,98(1/2):99-107.
[19] Collado R, Bermúdez-caraballoso I, García L R, et al. Epicotyl sections as targets for plant regeneration and transient transformation of common bean using Agrobacterium tumefaciens[J].In Vitro Cellular&Developmental Biology-Plant,2016,52(5):1-12.
[20] Mohamed M F, Cao J, Earle E D. Toward production of genetically modified common bean via Agrobacterium-mediated transformation[J].Annual REPORT-BEAN Improvement Cooperative,2006,49:147.
[21]刘宏宇,秦智伟.菜豆的组织培养、遗传转化及分子生物学研究进展[A].//蔬菜分子育种研讨会[C].北京,2004:6.
[22] Svetleva D, Velcheva M, Bhowmik G. Biotechnology as a useful tool in common bean(Phaseolus vulgaris L.)improvement[J].Euphytica,2003,131(2):189-200.
[23] Veltcheva M R, Lilova Svetleva D. In vitro regeneration of Phaseolus vulgaris L. via organogenesis from petiole explants[J].Journal of Central European Agriculture,2005,6(1):53-58.
[24] Ravnikar M, Rode J, Gogala N, et al. Regulation of organogenesis with jasmonic acid[C].1990:169-172.
[25] Benedicic D, Ravnikar M, Gogala N. The influence of jasmonic acid on the development of Phaseolus vulgaris shoot culture[A].International Symposium on Plant Biotechnology and its Contribution to Plant Development, Multiplication and Improvement 289[C].1991:85-86.
[26] Benedicic D, Ravnikar M, Gogala N. The regeneration of bean plants from meristem culture[J].Phyton Annales REI Botanicae,1997,37(1):151-160.
[27] Brasileiro A M, Arag?o F L, Rossi S, et al. Susceptibility of common and tepary beans to Agrobacterium spp. strains and improvement of Agrobacterium-mediated transformation using microprojectile bombardment[J].Journal of the American Society for Horticultural Science American Society for Horticultural Science,1996,121(5):810-815.
[28] Mohamed M F, Coyne D P, Read P E. Shoot organogenesis in callus induced from pedicel explants of common bean(Phaseolus vulgaris L.)[J].Journal of the American Society for Horticultural Science,1993,118(1):158-162.
[29] Arellano J, Fuentes S I, Castillo-espa?a P, et al. Regeneration of different cultivars of common bean(Phaseolus vulgaris L.)via indirect organogenesis[J].Plant Cell Tissue&Organ Culture,2009,96(1):11-18.
[30] Collado R, Veitía N, Bermúdezcaraballoso I, et al. Efficient in vitro plant regeneration via indirect organogenesis for different common bean cultivars[J].Scientia Horticulturae,2013,153(3):109-116.
[31]张倩.难以再生菜豆不定芽的诱导[D].南京:南京师范大学,2017.
[32] Allavena A, Rossetti L. Efforts in somatic embryogenesis of Phaseolus vulgaris L[J].ACTA Horticulturae,1982,131:239-246.
[33] Malik K A, Saxena P K. Somatic embryogenesis and shoot regeneration from intact seedlings of Phaseolus acutifolius A., P.aureus(L.)Wilczek, P. coccineus L., and P. wrightii L[J].Plant Cell Reports,1992,11(3):163-168.
[34] García L R, Pérez J, Kosky R G, et al. Plant regeneration via somatic embryogenesis in Phaseolus acutifolius A. Gray[J].Biotecnología Vegetal,2010,10(3):143-149.
[35] Collado R, García L R, Angenon G, et al. Somatic embryos formation from immature cotyledons in Phaseolus vulgaris cv.CIAP 7247[J].Biotecnología Vegetal,2011,11(4):235-240.
[36] Martins I S, Sondahl M R. Early stages of somatic embryo differentiation from callus cells of bean(Phaseolus vulgaris l.)grown in liquid medium[J].Journal of Plant Physiology,1984,117(2):97-103.
[37] Cabrera-ponce J L, López L, León-ramírez C G, et al. Stress induced acquisition of somatic embryogenesis in common bean Phaseolus vulgaris L[J].Protoplasma,2015,252(2):559-570.
[38] Mariotti D, Fontana G S, Santini L. Genetic transformation of grain legumes:Phaseolus vulgaris L. and P. coccineus L[J].J Genet Breed,1989,43:77-82.
[39] Dillen W, De Clercq J, Goossens A, et al. Exploiting the presence of regeneration capacity in the Phaseolus gene pool for Agrobacterium-mediated gene transfer to the common bean[J].Mededelingen Van DE Faculteit Landbouwkundige EN Toegepaste Biologische Wetenschappen, Universiteit GENT,1997,62(4a):1397-1402.
[40] Zhang Z, Coyne D P, Mitra A. Factors affecting Agrobacteriummediated transformation of common bean[J].Journal of the American Society for Horticultural Science American Society for Horticultural Science,1997,122(3):300-305.
[41] Estrada-navarrete G, Diaz-camino C, Santana O, et al.Agrobacterium rhizogenes transformation of the Phaseolus spp.:a tool for functional genomics[J].Molecular plant-microbe Interactions:MPMI,2006,19(12):1385-1393.
[42] Georgina E N, Xochitl A A, Juan-elías O, et al. Fast, efficient and reproducible genetic transformation of Phaseolus spp. by Agrobacterium rhizogenes[J].Nature Protocols,2007,2(7):1819-1824.
[43] Amugune N O, Anyango B, Mukiama T K. Arobacterium-mediated transformation of common bean[J].African Crop Science Journal,2011,19(3):137-147.
[44] Collado R, Bermúdez-caraballoso I, García L R, et al.Agrobacterium-mediated transformation of Phaseolus vulgaris L.using indirect organogenesis[J].Scientia Horticulturae,2015,195(27):89-100.
[45] Nagl W, Ignacimuthu S, Becker J. Genetic engineering and regeneration of Phaseolus and Vigna. State of the art and new attempts[J].Journal of Plant Physiology,1997,150(6):625-644.
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