内皮祖细胞外泌体中与血管生成相关的miRNAs生物信息学分析

详细信息    查看全文 | 推荐本文 |

英文篇名：Bioinformatics analysis of angiogenesis-related miRNAs in endothelial progenitor cell exosomes 作者：熊武 ; 孙安梦 ; 皇毅 ; 全文娟 ; 游凤姣 ; 杨旭龙 ; 兰宏伟 ; 苏红辉 ; 周建大 英文作者：Xiong Wu;Sun Anmeng;Huang Yi;Quan Wenjuan;You Fengjiao;Yang Xulong;Lan Hongwei;Su Honghui;Zhou Jianda;Department of Burn and Plastic Surgery,the First Affiliated Hospital of Hunan University of Chinese Medicine;Hunan University of Chinese Medicine;Department of Plastic and Cosmetic Surgery,the Third Xiangya Hospital of Central South University; 关键词：内皮祖细胞 ; 外泌体 ; 微RNAs ; 新生血管化 ; 生理性 ; 生物信息学 英文关键词：Endothelial progenitor cells;;Exosomes;;MicroRNAs;;Neovascularization,physiologic;;Bioinformatics 中文刊名：中国医师杂志 英文刊名：Journal of Chinese Physician 机构：湖南中医药大学第一附属医院烧伤疮疡整形外科;湖南中医药大学;中南大学湘雅三医院整形美容外科; 出版日期：2019-04-20 出版单位：中国医师杂志 年：2019 期：04 基金：湖南省卫生计生委科研计划课题项目(B20180739)~~ 语种：中文; 页：27-30 页数：4 CN：43-1274/R ISSN：1008-1372 分类号：R329.2

摘要

目的用生物信息学方法筛选内皮祖细胞(EPCs)外泌体miRNA用于调节血管生成的医学科学研究。方法从现有数据库中筛选人内皮祖细胞外泌体miRNAs,同时筛选与血管生成相关miRNAs,取两组miRNAs的交集,从而获得内皮祖细胞外泌体中与血管生成相关的候选miRNAs分子。结果通过实验数据分析和文献筛选获得的内皮祖细胞外泌体miRNAs分别为160个和50个,与血管生成相关miRNAs分子约600个;分别取频次较高的前20个,最终获得9个在内皮祖细胞外泌体中可能高表达且与血管生成相关的miRNAs:miR-126、miR-21、miR-221、miR-92a、miR-199a、miR-210、miR-214、miR-155、miR-146a。结论基于数据分析和文献筛选的生物信息学可简单而可靠地筛选目标miRNA进行后续研究,有较高推广应用价值。
        Objective By taking usage of bioinformatics screening methods,this medical research aimed at exploring how the miRNAs in endothelial progenitor cell exosomes relate to the regulation of angiogenesis. Methods miRNAs in endothelial progenitor cells exosomes and angiogenesis-related miRNA was intersected from the existing database to obtain the candidates of miRNA molecules related to angiogenesis in endothelial progenitor exosomes. Results 160 and 50 miRNAs in endothelial progenitor cell candidates were obtained through experimental data analysis and literature searching respectively. 600 candidates of angiogenesis-related miRNAs were obtained through literature searching; the top 20 with the highest frequency were selected out. Finally,9 miRNA candidates(miR-126,miR-21,miR-221,miR-92 a,miR-199 a,miR-210,miR-214,miR-155,miR-146 a) that may be highly expressed in endothelial progenitor exosomes and associated with angiogenesis were obtained for the following research. Conclusions Based on data analysis and literature searching,bioinformatics could screen out the target miRNAs for follow-up studies easily and reliably,it is worthy to be widely applied and popularized.

引文

[1] Ambros V. The functions of animal microRNAs[J]. Nature,2004,431(7006):350-355. DOI:10. 1038/nature02871.
    [2] Tiwari A,Mukherjee B,Dixit M. MicroRNA Key to Angiogenesis Regulation:MiRNA Biology and Therapy[J]. Curr Cancer Drug Targets,2018,18(3):266-277. DOI:10. 2174/1568009617666170630142725.
    [3] Li X,Chen C,Wei L,et al. Exosomes derived from endothelial progenitor cells attenuate vascular repair and accelerate reendothelialization by enhancing endothelial function[J]. Cytotherapy,2016,18(2):253-262. DOI:10. 1016/j. jcyt. 2015. 11. 009.
    [4] Zhou Y,Li P,Goodwin AJ,et al. Exosomes from Endothelial Progenitor Cells Improve the Outcome of a Murine Model of Sepsis[J]. Mol Ther,2018,26(5):1375-1384. DOI:10. 1016/j. ymthe.2018. 02. 020.
    [5]陈晓霞,唐宁宁,谢慧清,等.负压创面影响肉芽血管生成过程中miR-195和NLRX1表达研究[J].中国医师杂志,2018,20(2):183-186,190. DOI:10. 3760/cma. j. issn. 1008-1372. 2018. 02. 007.
    [6] Ferguson S,Kim S,Lee C,et al. The Phenotypic Effects of Exosomes Secreted from Distinct Cellular Sources:a Comparative Study Based on miRNA Composition[J]. AAPS J,2018,20(4):67. DOI:10. 1208/s12248-018-0227-4.
    [7] Zhang J,Chen C,Hu B,et al. Exosomes Derived from Human Endothelial Progenitor Cells Accelerate Cutaneous Wound Healing by Promoting Angiogenesis Through Erk1/2 Signaling[J]. Int J Biol Sci,2016,12(12):1472-1487. DOI:10. 7150/ijbs. 15514.
    [8] Li X,Jiang C,Zhao J. Human endothelial progenitor cells-derived exosomes accelerate cutaneous wound healing in diabetic rats by promoting endothelial function[J]. J Diabetes Complications,2016,30(6):986-992. DOI:10. 1016/j. jdiacomp. 2016. 05. 009.
    [9] Ragusa M,Barbagallo C,Statello L,et al. miRNA profiling in vitreous humor,vitreal exosomes and serum from uveal melanoma patients:Pathological and diagnostic implications[J]. Cancer Biol Ther,2015,16(9):1387-1396. DOI:10. 1080/15384047. 2015. 1046021.
    [10] Zhang H,Bai M,Deng T,et al. Cell-derived microvesicles mediate the delivery of miR-29a/c to suppress angiogenesis in gastric carcinoma[J]. Cancer Lett,2016,375(2):331-339. DOI:10.1016/j. canlet. 2016. 03. 026.