miR-126对男性喉癌患者进展影响的生物信息学分析
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摘要
目的通过预测分析miR-126的靶基因,为研究miR-126影响男性喉癌患者进程的作用机制提供理论支持。方法分析肿瘤基因组图谱(TCGA)数据库中miR-126对男性喉癌患者的具体影响,寻求miR-126影响男性喉癌患者的靶基因,通过DAVID数据库对有效靶基因进行基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析。结果 miR-126高表达可提高男性喉癌患者总生存率(OS),且在正常组织中高表达;miR-126的58个靶基因中有7个(DNMT1、GRIN2B、L2HGDH、PIK3R2、PTPN7、SIRT1、TWF1)对生存有影响;GO富集分析发现有效靶基因参与异染色质形成和细胞代谢活动;KEGG通路富集分析发现PIK3R2、SIRT1主要通过AMPK和FoxO信号通路影响细胞代谢和凋亡过程。结论 miR-126可以提高男性喉癌患者的OS,这种作用可能是通过其靶基因调节细胞代谢及凋亡过程实现的。
Objective To acquire theoretical data for studying the mechanism of miR-126 in male patients with laryngeal cancer, and predict and analyze the target gene of miR-126. Methods The cancer Genome Atlas(TCGA)database was analyzed for specific effects of miR-126 on male patients with laryngeal cancer. And then the target gene of miR-126 was predicted by target gene databases of male patients with laryngeal cancer. The gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment was analyzed by DAVID database. Results The high expression of miR-126 was beneficial to increase the overall survival(OS) of patients with laryngeal cancer. And the higher expression of miR-126 was found in normal tissue. Seven of 58 target genes(DNMT1, GRIN2 B, L2 HGDH, PIK3 R2, PTPN7,SIRT1, TWF1) worked in effecting the overall survival of patients with laryngeal cancer. GO enrichment analysis found that target genes constituted the heterochromatin, and involved in cell metabolism. KEGG pathway enrichment analysis found that PIK3 R2 and SIRT1 took part in cell metabolism and apoptosis by AMPK and FoxO signal pathways. Conclusion MiR-126 could improve the OS of male patients with laryngeal cancer, which is achieved by regulating gene expression, cell metabolism and apoptosis.
Objective To acquire theoretical data for studying the mechanism of miR-126 in male patients with laryngeal cancer, and predict and analyze the target gene of miR-126. Methods The cancer Genome Atlas(TCGA)database was analyzed for specific effects of miR-126 on male patients with laryngeal cancer. And then the target gene of miR-126 was predicted by target gene databases of male patients with laryngeal cancer. The gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment was analyzed by DAVID database. Results The high expression of miR-126 was beneficial to increase the overall survival(OS) of patients with laryngeal cancer. And the higher expression of miR-126 was found in normal tissue. Seven of 58 target genes(DNMT1, GRIN2 B, L2 HGDH, PIK3 R2, PTPN7,SIRT1, TWF1) worked in effecting the overall survival of patients with laryngeal cancer. GO enrichment analysis found that target genes constituted the heterochromatin, and involved in cell metabolism. KEGG pathway enrichment analysis found that PIK3 R2 and SIRT1 took part in cell metabolism and apoptosis by AMPK and FoxO signal pathways. Conclusion MiR-126 could improve the OS of male patients with laryngeal cancer, which is achieved by regulating gene expression, cell metabolism and apoptosis.
引文
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[7]Jia Z,Zhang Y,Xu Q,et al.miR-126 suppresses epithelial-tomesenchymal transition and metastasis by targeting PI3K/AKT/Snail signaling of lung cancer cells[J].Oncol Lett,2018,15(5):7369-7375.doi:10.3892/ol.2018.8207.
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[9]Jili S,Eryong L,Lijuan L,et al.RUNX3 inhibits laryngeal squamous cell carcinoma malignancy under the regulation of miR-148a-3p/DNMT1 axis[J].Cell Biochem Funct,2016,34(8):597-605.doi:10.1002/cbf.3233.
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