Internal phosphate starvation signaling and external phosphate availability have no obvious effect on the accumulation of cadmium in rice
|
摘要
Phosphorus(P) is a limiting nutrient element for crop. To obtain maximum crop yield, P fertilizer is often over-applied, which leads to accelerating exhaustion of phosphate resources and serious environmental problems. Reducing the application of P fertilizer and enhancing the P utilization efficiency of crops are significant for the sustainable development of agriculture. Cadmium(Cd) contamination in rice is another serious agricultural issue. However, whether reducing the application of P fertilizer and enhancing the P utilization efficiency of crops will increase the risk of Cd accumulation in crops remains obscure. In this study, we are aiming to elucidate the relationship between Cd and P in rice from physiological and genetic perspectives. For this purpose, the wild type(WT) rice plants and phosphate(Pi)-starvation signaling repressed mutant phr2 were used to analyze the relationship between Cd and P. Here, we found that Cd stress could promote P accumulation and induce Pi-starvation signaling in WT and phr2 shoots under Pi-sufficient condition in a PHOSPHATE STARVATION RESPONSE 2(PHR2) independent manner. Besides, the expression level of Cd transporter of OsNramp5 and the uptake speed of Cd~(2+) were not obviously changed under Pi-sufficient and Pi-deficient conditions. Furthermore, our Cd determination results showed that the Cd concentrations in WT and phr2 were not obviously changed under Pi-sufficient and Pi-deficient conditions. These results indicate that the external P availability and internal Pi-starvation signaling cannot obviously affect the accumulation of Cd in rice seedling.
Phosphorus(P) is a limiting nutrient element for crop. To obtain maximum crop yield, P fertilizer is often over-applied, which leads to accelerating exhaustion of phosphate resources and serious environmental problems. Reducing the application of P fertilizer and enhancing the P utilization efficiency of crops are significant for the sustainable development of agriculture. Cadmium(Cd) contamination in rice is another serious agricultural issue. However, whether reducing the application of P fertilizer and enhancing the P utilization efficiency of crops will increase the risk of Cd accumulation in crops remains obscure. In this study, we are aiming to elucidate the relationship between Cd and P in rice from physiological and genetic perspectives. For this purpose, the wild type(WT) rice plants and phosphate(Pi)-starvation signaling repressed mutant phr2 were used to analyze the relationship between Cd and P. Here, we found that Cd stress could promote P accumulation and induce Pi-starvation signaling in WT and phr2 shoots under Pi-sufficient condition in a PHOSPHATE STARVATION RESPONSE 2(PHR2) independent manner. Besides, the expression level of Cd transporter of OsNramp5 and the uptake speed of Cd~(2+) were not obviously changed under Pi-sufficient and Pi-deficient conditions. Furthermore, our Cd determination results showed that the Cd concentrations in WT and phr2 were not obviously changed under Pi-sufficient and Pi-deficient conditions. These results indicate that the external P availability and internal Pi-starvation signaling cannot obviously affect the accumulation of Cd in rice seedling.
Phosphorus(P) is a limiting nutrient element for crop. To obtain maximum crop yield, P fertilizer is often over-applied, which leads to accelerating exhaustion of phosphate resources and serious environmental problems. Reducing the application of P fertilizer and enhancing the P utilization efficiency of crops are significant for the sustainable development of agriculture. Cadmium(Cd) contamination in rice is another serious agricultural issue. However, whether reducing the application of P fertilizer and enhancing the P utilization efficiency of crops will increase the risk of Cd accumulation in crops remains obscure. In this study, we are aiming to elucidate the relationship between Cd and P in rice from physiological and genetic perspectives. For this purpose, the wild type(WT) rice plants and phosphate(Pi)-starvation signaling repressed mutant phr2 were used to analyze the relationship between Cd and P. Here, we found that Cd stress could promote P accumulation and induce Pi-starvation signaling in WT and phr2 shoots under Pi-sufficient condition in a PHOSPHATE STARVATION RESPONSE 2(PHR2) independent manner. Besides, the expression level of Cd transporter of OsNramp5 and the uptake speed of Cd~(2+) were not obviously changed under Pi-sufficient and Pi-deficient conditions. Furthermore, our Cd determination results showed that the Cd concentrations in WT and phr2 were not obviously changed under Pi-sufficient and Pi-deficient conditions. These results indicate that the external P availability and internal Pi-starvation signaling cannot obviously affect the accumulation of Cd in rice seedling.
引文
Ahonen-Jonnarth U,Finlay R D.2001.Effects of elevated nickel and cadmium concentrations on growth and nutrient uptake of mycorrhizal and non-mycorrhizal Pinus sylvestris seedlings.Plant and Soil,236,129-138.
Bustos B,Castrillo G,Linhares F,Puga M I,Rubio V,PerezPerez J,Solano R,Leyva A,Paz-Ares J.2010.A central regulatory system largely controls transcriptional activation and repression responses to phosphate starvation in Arabidopsis.PLo S Genetics,6,e1001102.
Cheng L,Bucciarelli B,Liu J,Zinn K,Miller S,Patton-Vogt J,Allan D,Shen J,Vance C P.2011.White lupin cluster root acclimation to phosphorus deficiency and root hair development involve unique glycerophosphodiester phosphodiesterases.Plant Physiology,156,1131-1148.
Conley D J,Paerl H W,Howarth R W,Boesch D F,Seitzinger S P,Havens K E,Lancelot C,Likens G E.2009.Controlling eutrophication:Nitrogen and phosphorus.Science,323,1014-1015.
Deng L,Xia J,Zhang Y,Lei Z,Lu Y.2013.Effects of cadmium pollution in soil on contents and accumulation amounts of nitrogen,phosphorus,potassium and cadmium elements in potato tubers.Agricultural Science and Technology,14,1280-1284.
Dong J,Mao W H,Zhang G P,Wu F B,Cai Y.2007.Root excretion and plant tolerance to cadmium toxicity-a review.Plant,Soil and Environment,53,193-200.
Fan J L,Hu Z Y,Ziadi N,Xia X,Wu C Y H.2009.Excessive sulfur supply reduces cadmium accumulation in brown rice(Oryza sativa L.).Environmental Pollution,158,409-415.
Franco-Zorrilla J M,Gonzalez E,Bustos R,Linhares F,Leyva A,Paz-Ares J.2004.The transcriptional control of plant responses to phosphate limitation.Journal of Experimental Botany,55,285-293.
Guo M N,Ruan W Y,Li C Y,Huang F L,Zeng M,Liu Y Y,Yu Y,Ding X M,Wu Y R,Wu Z C,Mao C Z,Yi K K,Wu P,Mo X R.2015.Integrative comparison of the role of the PHOSPHATE RESPONSE1 subfamily in phosphate signaling and homeostasis in rice.Plant Physiology,168,1762-1776.
Han L N,Ju X H,Zhang C B,Song Z G,Wang J A,Liu Z Q.2014.Genotypic variation of Cd2+flux and its relationship with Cd accumulation in rice plant.Journal of Agro-Environment Science,33,37-42.(in Chinese)
Ishimaru Y,Suzuki M,Tsukamoto T,Suzuki K,Nakazono M,Kobayashi T,Wada Y,Watanabe S,Matsuhashi S,Takahashi M,Nakanishi H,Mori S,Nishizawa N K.2006.Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+.The Plant Journal,45,335-346.
J?rup L,?kesson A.2009.Current status of cadmium as an environmental health problem.Toxicology and Applied Pharmacology,238,201-208.
López-Arredondo D L,Leyva-González M A,González-Morales S I,López-Bucio J,Herrera-Estrella L.2014.Phosphate nutrition:Improving low-phosphate tolerance in crops.Annual Review of Plant Biology,65,95-123.
Marschner H.1995.Mineral nutrition of higher plants.Journal of Ecology,76,1250.
Misson,J,Raghothama K G,Jain A,Jouhet J,Block M A,Bligny R,Ortet P,Creff A,Somerville S,Roland N,Doumas P,Nacry P,Herrerra-Estrella L,Nussaume L,Thibaud M.2005.A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation.Proceedings of the National Academy of Sciences of the United States of America,102,11934-11939.
Mulligan C N,Yong R N,Gibbs B F.2001.Remediation technologies for metal contaminated soils and groundwater:An evaluation.Engineering Geology,60,193-207.
Nakanishi H,Ogawa I,Ishimaru Y,Mori S,Nishizawa N K.2006.Iron deficiency enhances cadmium uptake and translocation mediated by the Fe2+transporters Os IRT1 and Os IRT2 in rice.Soil Science and Plant Nutrition,52,464-469.
Qiu Q,Wang Y T,Yang Z Y,Yuan J G.2011.Effects of phosphorus supplied in soil on subcellular distribution and chemical forms of cadmium in two Chinese flowering cabbage(Brassica parachinensis L.)cultivars differing in cadmium accumulation.Food and Chemical Toxicology,49,2260-2267.
Qiu Z Z,Guan Z Y,Long C Y.2005.Effect of zinc on cadmium uptake by spring wheat(Triticum aestivum L.):Long time hydroponic study and short time 109Cd tracing study.Journal of Zhejiang University Science,6,643-648.
Rizwan M,Meunier J D,Miche H,Keller C.2012.Effect of silicon on reducing cadmium toxicity in durum wheat(Triticum turgidum L.cv.Claudio W.)grown in a soil with aged contamination.Journal of Hazardous Materials,209-210,326-334.
Rubio V,Linhares F,Solano R,Martin A C,Iglesias J,Leyva A,Paz-Ares J.2001.A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae.Genes&Development,15,2122-2133.
Sajwan K S,Paramasivam S,Richardson J P,Alva A K.2002.Phosphorus alleviation of cadmium phytotoxicity.Journal of Plant Nutrition,25,2027-2034.
Sanita di Toppi L,Gabbrielli R.1999.Response to cadmium in higher plants.Environmental and Experimental Botany,41,105-130.
Sasaki A,Yamaji N,Yokosho K,Ma J F.2012.Nramp5 is a major transporter responsible for manganese and cadmium uptake in rice.The Plant Cell,24,2155-2167.
Shao G,Chen M,Wang W,Mou R,Zhang G.2007.Iron nutrition affects cadmium accumulation and toxicity in rice plants.Plant Growth Regulation,53,33-42.
Tian S,Lu L,Zhang J,Wang K,Brown P,He Z,Liang J,Yang X.2011.Calcium protects roots of Sedum alfredii H.against cadmium-induced oxidative stress.Chemosphere,84,63-69.
Uraguchi S,Mori S,Kuramata M,Kawasaki A,Arao T,Ishikawa S.2009.Root-to-shoot Cd translocation via the xylem is the major process determining shoot and grain cadmium accumulation in rice.Journal of Experimental Botany,60,2677-2688.
Wu D,Yamaji N,Yamane M,Kashino-Fujii M,Sato K,Ma JF.2016.The Hv Nramp5 transporter mediates uptake of cadmium and manganese,but not iron.Plant Physiology,172,1899-1910.
Xu J,Sun J H,Du L G,Liu X J.2012.Comparative transcriptome analysis of cadmium responses in Solanum nigrum and Solanum torvum.New Phytologist,196,110-124.
Yang D,Guo Z,Green I D,Xie D.2016.Effect of cadmium accumulation on mineral nutrient levels in vegetable crops:Potential implications for human health.Environmental Science&Pollution Research,23,19744-19753.
Yang Y,Chen R,Fu G,Xiong J,Tao L.2016.Phosphate deprivation decreases cadmium(Cd)uptake but enhances sensitivity to Cd by increasing iron(Fe)uptake and inhibiting phytochelatins synthesis in rice(Oryza sativa).Acta Physiologiae Plantarum,38,28.
Yin A,Yang Z,Ebbs S,Yuan J,Wang J,Yang J.2016.Effects of phosphorus on chemical forms of Cd in plants of four spinach(Spinacia oleracea L.)cultivars differing in Cd accumulation.Environmental Science and Pollution Research,23,5753-5762.
Yoshida S,Forno D A,Cock J H,Gomez K A.1976.Laboratory Manual for Physiological Studies of Rice.3rd ed.International Rice Research Institute,Manila,the Philippines.
Zhao X,Jiang T,Du B.2014.Effect of organic matter and calcium carbonate on behaviors of cadmium adsorptiondesorption on/from purple paddy soils.Chemosphere,99,41-48.
Zheng L,Huang F,Narsai R,Wu J,Girand E,He F,Cheng L,Wang F,Wu P,Whelan J,Shou H.2009.Physiological and transcriptome analysis of iron and phosphorous interaction in rice seedlings.Plant Physiology,151,262-274.
Zhou J,Jiao F C,Wu Z C,Li Y Y,Wang X M,He X W,Zhong WQ,Wu P.2008.Os PHR2 is involved in phosphate-starvation signaling and excessive phosphate accumulation in shoots of plants.Plant Physiology,146,1673-1686.
Zhu X F,Lei G J,Jiang T,Liu Y,Li G X,Zheng S J.2012.Cell wall polysaccharides are involved in P-deficiency-induced Cd exclusion in Arabidopsis thaliana.Planta,236,989-997.
Bustos B,Castrillo G,Linhares F,Puga M I,Rubio V,PerezPerez J,Solano R,Leyva A,Paz-Ares J.2010.A central regulatory system largely controls transcriptional activation and repression responses to phosphate starvation in Arabidopsis.PLo S Genetics,6,e1001102.
Cheng L,Bucciarelli B,Liu J,Zinn K,Miller S,Patton-Vogt J,Allan D,Shen J,Vance C P.2011.White lupin cluster root acclimation to phosphorus deficiency and root hair development involve unique glycerophosphodiester phosphodiesterases.Plant Physiology,156,1131-1148.
Conley D J,Paerl H W,Howarth R W,Boesch D F,Seitzinger S P,Havens K E,Lancelot C,Likens G E.2009.Controlling eutrophication:Nitrogen and phosphorus.Science,323,1014-1015.
Deng L,Xia J,Zhang Y,Lei Z,Lu Y.2013.Effects of cadmium pollution in soil on contents and accumulation amounts of nitrogen,phosphorus,potassium and cadmium elements in potato tubers.Agricultural Science and Technology,14,1280-1284.
Dong J,Mao W H,Zhang G P,Wu F B,Cai Y.2007.Root excretion and plant tolerance to cadmium toxicity-a review.Plant,Soil and Environment,53,193-200.
Fan J L,Hu Z Y,Ziadi N,Xia X,Wu C Y H.2009.Excessive sulfur supply reduces cadmium accumulation in brown rice(Oryza sativa L.).Environmental Pollution,158,409-415.
Franco-Zorrilla J M,Gonzalez E,Bustos R,Linhares F,Leyva A,Paz-Ares J.2004.The transcriptional control of plant responses to phosphate limitation.Journal of Experimental Botany,55,285-293.
Guo M N,Ruan W Y,Li C Y,Huang F L,Zeng M,Liu Y Y,Yu Y,Ding X M,Wu Y R,Wu Z C,Mao C Z,Yi K K,Wu P,Mo X R.2015.Integrative comparison of the role of the PHOSPHATE RESPONSE1 subfamily in phosphate signaling and homeostasis in rice.Plant Physiology,168,1762-1776.
Han L N,Ju X H,Zhang C B,Song Z G,Wang J A,Liu Z Q.2014.Genotypic variation of Cd2+flux and its relationship with Cd accumulation in rice plant.Journal of Agro-Environment Science,33,37-42.(in Chinese)
Ishimaru Y,Suzuki M,Tsukamoto T,Suzuki K,Nakazono M,Kobayashi T,Wada Y,Watanabe S,Matsuhashi S,Takahashi M,Nakanishi H,Mori S,Nishizawa N K.2006.Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+.The Plant Journal,45,335-346.
J?rup L,?kesson A.2009.Current status of cadmium as an environmental health problem.Toxicology and Applied Pharmacology,238,201-208.
López-Arredondo D L,Leyva-González M A,González-Morales S I,López-Bucio J,Herrera-Estrella L.2014.Phosphate nutrition:Improving low-phosphate tolerance in crops.Annual Review of Plant Biology,65,95-123.
Marschner H.1995.Mineral nutrition of higher plants.Journal of Ecology,76,1250.
Misson,J,Raghothama K G,Jain A,Jouhet J,Block M A,Bligny R,Ortet P,Creff A,Somerville S,Roland N,Doumas P,Nacry P,Herrerra-Estrella L,Nussaume L,Thibaud M.2005.A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation.Proceedings of the National Academy of Sciences of the United States of America,102,11934-11939.
Mulligan C N,Yong R N,Gibbs B F.2001.Remediation technologies for metal contaminated soils and groundwater:An evaluation.Engineering Geology,60,193-207.
Nakanishi H,Ogawa I,Ishimaru Y,Mori S,Nishizawa N K.2006.Iron deficiency enhances cadmium uptake and translocation mediated by the Fe2+transporters Os IRT1 and Os IRT2 in rice.Soil Science and Plant Nutrition,52,464-469.
Qiu Q,Wang Y T,Yang Z Y,Yuan J G.2011.Effects of phosphorus supplied in soil on subcellular distribution and chemical forms of cadmium in two Chinese flowering cabbage(Brassica parachinensis L.)cultivars differing in cadmium accumulation.Food and Chemical Toxicology,49,2260-2267.
Qiu Z Z,Guan Z Y,Long C Y.2005.Effect of zinc on cadmium uptake by spring wheat(Triticum aestivum L.):Long time hydroponic study and short time 109Cd tracing study.Journal of Zhejiang University Science,6,643-648.
Rizwan M,Meunier J D,Miche H,Keller C.2012.Effect of silicon on reducing cadmium toxicity in durum wheat(Triticum turgidum L.cv.Claudio W.)grown in a soil with aged contamination.Journal of Hazardous Materials,209-210,326-334.
Rubio V,Linhares F,Solano R,Martin A C,Iglesias J,Leyva A,Paz-Ares J.2001.A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae.Genes&Development,15,2122-2133.
Sajwan K S,Paramasivam S,Richardson J P,Alva A K.2002.Phosphorus alleviation of cadmium phytotoxicity.Journal of Plant Nutrition,25,2027-2034.
Sanita di Toppi L,Gabbrielli R.1999.Response to cadmium in higher plants.Environmental and Experimental Botany,41,105-130.
Sasaki A,Yamaji N,Yokosho K,Ma J F.2012.Nramp5 is a major transporter responsible for manganese and cadmium uptake in rice.The Plant Cell,24,2155-2167.
Shao G,Chen M,Wang W,Mou R,Zhang G.2007.Iron nutrition affects cadmium accumulation and toxicity in rice plants.Plant Growth Regulation,53,33-42.
Tian S,Lu L,Zhang J,Wang K,Brown P,He Z,Liang J,Yang X.2011.Calcium protects roots of Sedum alfredii H.against cadmium-induced oxidative stress.Chemosphere,84,63-69.
Uraguchi S,Mori S,Kuramata M,Kawasaki A,Arao T,Ishikawa S.2009.Root-to-shoot Cd translocation via the xylem is the major process determining shoot and grain cadmium accumulation in rice.Journal of Experimental Botany,60,2677-2688.
Wu D,Yamaji N,Yamane M,Kashino-Fujii M,Sato K,Ma JF.2016.The Hv Nramp5 transporter mediates uptake of cadmium and manganese,but not iron.Plant Physiology,172,1899-1910.
Xu J,Sun J H,Du L G,Liu X J.2012.Comparative transcriptome analysis of cadmium responses in Solanum nigrum and Solanum torvum.New Phytologist,196,110-124.
Yang D,Guo Z,Green I D,Xie D.2016.Effect of cadmium accumulation on mineral nutrient levels in vegetable crops:Potential implications for human health.Environmental Science&Pollution Research,23,19744-19753.
Yang Y,Chen R,Fu G,Xiong J,Tao L.2016.Phosphate deprivation decreases cadmium(Cd)uptake but enhances sensitivity to Cd by increasing iron(Fe)uptake and inhibiting phytochelatins synthesis in rice(Oryza sativa).Acta Physiologiae Plantarum,38,28.
Yin A,Yang Z,Ebbs S,Yuan J,Wang J,Yang J.2016.Effects of phosphorus on chemical forms of Cd in plants of four spinach(Spinacia oleracea L.)cultivars differing in Cd accumulation.Environmental Science and Pollution Research,23,5753-5762.
Yoshida S,Forno D A,Cock J H,Gomez K A.1976.Laboratory Manual for Physiological Studies of Rice.3rd ed.International Rice Research Institute,Manila,the Philippines.
Zhao X,Jiang T,Du B.2014.Effect of organic matter and calcium carbonate on behaviors of cadmium adsorptiondesorption on/from purple paddy soils.Chemosphere,99,41-48.
Zheng L,Huang F,Narsai R,Wu J,Girand E,He F,Cheng L,Wang F,Wu P,Whelan J,Shou H.2009.Physiological and transcriptome analysis of iron and phosphorous interaction in rice seedlings.Plant Physiology,151,262-274.
Zhou J,Jiao F C,Wu Z C,Li Y Y,Wang X M,He X W,Zhong WQ,Wu P.2008.Os PHR2 is involved in phosphate-starvation signaling and excessive phosphate accumulation in shoots of plants.Plant Physiology,146,1673-1686.
Zhu X F,Lei G J,Jiang T,Liu Y,Li G X,Zheng S J.2012.Cell wall polysaccharides are involved in P-deficiency-induced Cd exclusion in Arabidopsis thaliana.Planta,236,989-997.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |