弱光对黄瓜幼苗叶片光合产物积累的影响
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摘要
为阐明弱光胁迫对黄瓜光合产物积累的影响,选取4份弱光耐受性不同的黄瓜品系M14、M21、M61和M67作为试验材料,在幼苗2叶1心期进行弱光处理,弱光强度为50μmol·m~(-2)·s~(-1),对照光强为800μmol·m~(-2)·s~(-1),测定对照及弱光处理下叶片中糖及淀粉含量。结果表明,弱光处理对黄瓜幼苗叶片中光合产物存在显著影响,且弱光耐受性不同的黄瓜品系间存在着显著差异。与正常光相比,弱光处理3 d后幼苗叶片中可溶性糖含量、果糖含量和葡萄糖含量开始下降,到植株半致死状态时降幅最大,不耐弱光品系M14幼苗叶片中可溶性糖等含量较耐弱光品系M67降低的幅度更大。弱光处理后叶片中蔗糖、淀粉含量降低,不弱光品系叶片中蔗糖降低开始的时间较早,且降低的幅度大为78.23%,耐弱光品系幼苗叶片中蔗糖降低开始的时间较晚,幅度较小,弱光处理后期蔗糖降低幅度减小。为弱光胁迫下黄瓜叶片光合产物积累及代谢的生理机制提供研究基础。
In order to illuminate effects of low-light stress on the accumulation of photosynthetic product in cucumber leaves,4different genotypes of cucumber strains M14,M21,M61 and M67 with distinct tolerance ability to low-light stress were selected as the experimental materials.The low-light treatment was carried out when the seedlings grew to stage of 2 leaves and 1 heart.The lowlight stress was simulated in an artificial climate box.The low light intensity was set to 50μmol·m~(-2)·s~(-1),and the control light intensity was 800μmol·m~(-2)·s~(-1),the content of sugar and starch in leaves was measured.Results showed that the accumulation of photosynthetic product in cucumber leaves was affected obviously by low-light treatment and reached a significant level in different cucumber lines.Compared to the normal light,the decrease content of soluble sugar,fructose and glucose began after 3 days treatment,the biggest decline happened at half lethal stage.The decline scale of low-light sensitive strains(M14)was bigger than low-light tolerance strains(M67).The content of sucrose and starch was declined in cucumber leaves under low-light treatment,and it happened earlier in sensitive strains than tolerance strains with bigger scale of decline with 78.23%.It would provide fundamental basis for illuminating the accumulation of photosynthetic product and photosynthetic physiology mechanism under low-light stress.
In order to illuminate effects of low-light stress on the accumulation of photosynthetic product in cucumber leaves,4different genotypes of cucumber strains M14,M21,M61 and M67 with distinct tolerance ability to low-light stress were selected as the experimental materials.The low-light treatment was carried out when the seedlings grew to stage of 2 leaves and 1 heart.The lowlight stress was simulated in an artificial climate box.The low light intensity was set to 50μmol·m~(-2)·s~(-1),and the control light intensity was 800μmol·m~(-2)·s~(-1),the content of sugar and starch in leaves was measured.Results showed that the accumulation of photosynthetic product in cucumber leaves was affected obviously by low-light treatment and reached a significant level in different cucumber lines.Compared to the normal light,the decrease content of soluble sugar,fructose and glucose began after 3 days treatment,the biggest decline happened at half lethal stage.The decline scale of low-light sensitive strains(M14)was bigger than low-light tolerance strains(M67).The content of sucrose and starch was declined in cucumber leaves under low-light treatment,and it happened earlier in sensitive strains than tolerance strains with bigger scale of decline with 78.23%.It would provide fundamental basis for illuminating the accumulation of photosynthetic product and photosynthetic physiology mechanism under low-light stress.
引文
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[13]李丹丹,司龙亭,罗晓梅,等.弱光胁迫下黄瓜苗期下胚轴性状的遗传分析[J].西北农林科技大学学报:自然科学版,2009,37(11):113-119.
[14]高凌娜,司龙亭,李丹丹,等.弱光胁迫下黄瓜幼苗叶片解剖结构及光合特性研究[J].江西农业大学学报,2009,31(6):1011-1015.
[15]李丹丹,崔洪秋,刘芳,等.弱光胁迫下黄瓜幼苗霜霉病抗性评价与分析[J].植物保护学报,2016,43(2):113-118.
[16]张冠英,司龙亭,李丹丹.弱光胁迫下黄瓜幼苗下胚轴性状QTL分析[J].园艺学报,2011,38(2):295-302.
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[18]周艳虹,黄黎锋,喻景权.持续低温弱光对黄瓜叶片气体交换及叶绿素荧光淬灭和吸收光能分配的影响[J].植物生理与分子生物学报,2004,50(2):153-160.
[19]王永健,张海英,张峰,等.低温弱光对不同黄瓜品种幼苗光合作用的影响[J].园艺学报,2001,28(3):230-234.
[20]王海涛,秦智伟,李丹丹,等.黄瓜耐弱光性鉴定指标研究及其苗期与成株期的相关分析[J].中国蔬菜,2016(8):35-39.
[21]Beale SI.Enzymes of Chlorophyll Biosynthesis[J].Photosynthesis Research,1999,60(1):43-73.
[22]王伟,李明,张文慧.不同改良措施对盐碱地芸豆生长及产量的影响[J].黑龙江八一农垦大学学报,2018,30(1):1-7.
[23]李丹丹,司龙亭,牛海臣,等.黄瓜耐弱光性的多元统计分析[J].园艺学报,2009,36(4):501-506.
[2]艾希珍,马兴庄,于立明,等.弱光下长期亚适温和短期低温对黄瓜生长及光合作用的影响[J].应用生态学报,2004(11):2091-2094.
[3]Franklin KA.Shade avoidance[J].New Phytol,2008,179:930-944.
[4]吴学红,高杰.洋葱春化处理及可溶性糖和可溶性蛋白质变化规律的研究[J].新疆农业科学,2013(2):294-299.
[5]陈青君,张福墁,王永健,等.黄瓜对低温弱光反应的生理特征研究[J].中国农业科学,2003,36(1):77-81.
[6]李丹丹,邹士成,刘思源.低温弱光对黄瓜幼苗生长发育的影响[J].北方园艺,2015(18):41-43.
[7]米国全,刘丽英,金宝燕,等.弱光对不同生态型黄瓜幼苗光合速率及蔗糖代谢相关酶活性的影响[J].华北农学报,2011,26(1):146-150.
[8]李志刚,侯扶江,安渊,等.不同光照强度对三种牧草生长发育的影响[J].中国草地学报,2009(31):7.
[9]马德华,庞金安,霍振荣,等.弱光对黄瓜幼苗光合及膜脂过氧化作用的影响[J].河南农业大学学报,1998,32(1):3-5.
[10]Jang Y A,Goto E,Ishigami Y,et al.Effect of light intensity and relative humidity on photosynthesis,growth and grafttake of grafted cucumber seedlings during healing and acclimatization[J].Hort Environ Biotechnol,2011,52:331-338.
[11]战吉成,黄卫东,王利军.植物弱光逆境生理研究综述[J].植物学通报,2003,20(1):43-50.
[12]Li D D,Qin Z W,Lian H,et al.Inheritance and quantitative trait loci analysis of low-light tolerance in cucumber(Cucumis sativus L.)[J].Genetics and Molecular Research,2015(9):10609-10618.
[13]李丹丹,司龙亭,罗晓梅,等.弱光胁迫下黄瓜苗期下胚轴性状的遗传分析[J].西北农林科技大学学报:自然科学版,2009,37(11):113-119.
[14]高凌娜,司龙亭,李丹丹,等.弱光胁迫下黄瓜幼苗叶片解剖结构及光合特性研究[J].江西农业大学学报,2009,31(6):1011-1015.
[15]李丹丹,崔洪秋,刘芳,等.弱光胁迫下黄瓜幼苗霜霉病抗性评价与分析[J].植物保护学报,2016,43(2):113-118.
[16]张冠英,司龙亭,李丹丹.弱光胁迫下黄瓜幼苗下胚轴性状QTL分析[J].园艺学报,2011,38(2):295-302.
[17]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[18]周艳虹,黄黎锋,喻景权.持续低温弱光对黄瓜叶片气体交换及叶绿素荧光淬灭和吸收光能分配的影响[J].植物生理与分子生物学报,2004,50(2):153-160.
[19]王永健,张海英,张峰,等.低温弱光对不同黄瓜品种幼苗光合作用的影响[J].园艺学报,2001,28(3):230-234.
[20]王海涛,秦智伟,李丹丹,等.黄瓜耐弱光性鉴定指标研究及其苗期与成株期的相关分析[J].中国蔬菜,2016(8):35-39.
[21]Beale SI.Enzymes of Chlorophyll Biosynthesis[J].Photosynthesis Research,1999,60(1):43-73.
[22]王伟,李明,张文慧.不同改良措施对盐碱地芸豆生长及产量的影响[J].黑龙江八一农垦大学学报,2018,30(1):1-7.
[23]李丹丹,司龙亭,牛海臣,等.黄瓜耐弱光性的多元统计分析[J].园艺学报,2009,36(4):501-506.