栽培肾茶遗传多样性的SRAP标记分析
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摘要
应用SRAP分子标记技术对云南、广西、福建、海南等地收集的16个栽培肾茶种质进行遗传多样性分析。15对引物经PCR扩增后共检测到124个条带,其中多态性条带80个,多态性条带百分率(PPL)为66.7%;肾茶种质间的观测等位基因数(Na)为1.669 4、有效等位基因数(Ne)为1.434 0,Nei's基因多样性指数(H)为0.252 9,Shannon信息指数(I)为0.374 7,遗传相似度介于0.566 9到0.991 7之间,说明肾茶的遗传多样性水平较低。UPGMA聚类结果将肾茶种质分为2类,与根据花冠颜色对肾茶类型进行划分的结果对应,而遗传特性与地理分布没有明显相关性。实验结果可为肾茶遗传研究、品种选育与资源保护提供科学依据。
The genetic diversity of 16 cultivated Clerodendranthus spicatus germplasm, collected from Yunnan,Guangxi, Fujian and Hainan province, was analyzed by SRAP molecular marker technology. A total of 124 sites were detected after amplification with 15 pairs of primer combinations, with the number and percentage of polymorphic loci being 80 and 66.67%. Among the germplasm resources of Clerodendranthus spicatus, the number of Na, Ne, H and I were 1.669 4, 1.434 0, 0.252 9 and 0.374 7, respectively, the similarity coefficient ranged from0.566 9 to 0.991 7, which showed a low-level genetic diversity of C. spicatus. C. spicatus germplasm resources were classified into 2 categories based on the UPGMA cluster analysis, which corresponded with the classification by the color of corolla, meanwhile, hereditary characters were not related to the geographical distribution. The results could provide scientific basis for the genetics research, variety breeding and resource conservation of C.spicatus.
The genetic diversity of 16 cultivated Clerodendranthus spicatus germplasm, collected from Yunnan,Guangxi, Fujian and Hainan province, was analyzed by SRAP molecular marker technology. A total of 124 sites were detected after amplification with 15 pairs of primer combinations, with the number and percentage of polymorphic loci being 80 and 66.67%. Among the germplasm resources of Clerodendranthus spicatus, the number of Na, Ne, H and I were 1.669 4, 1.434 0, 0.252 9 and 0.374 7, respectively, the similarity coefficient ranged from0.566 9 to 0.991 7, which showed a low-level genetic diversity of C. spicatus. C. spicatus germplasm resources were classified into 2 categories based on the UPGMA cluster analysis, which corresponded with the classification by the color of corolla, meanwhile, hereditary characters were not related to the geographical distribution. The results could provide scientific basis for the genetics research, variety breeding and resource conservation of C.spicatus.
引文
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Gao L.X.,Liu N.,Huang B.H.,and Hu X.,2008,Phylogenetic analysis and genetic mapping of Chinese Hedychium using SRAP markers,Sci.Hortic.,117(4):369-377
Li A.X.,Liu Q.C.,Wang Q.M.,Zhang L.M.,Zhai H.,and Liu S.Z.,2010,Con struction of molecular linkage maps using SRAP markers in Sweetpotato,Acta Agronomica Sinica,36(8):1286-1295
Li G.,and Quiros C.F.,2001,Sequence-related amplified poly morphism(SRAP),a new marker system based on a simple PCR reaction:Its application to mapping and gene tagging in Brassica,Theor.Appl.Genet.,103(3):455-461
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Tao A.F.,Wei J.J.,Liu X.,Xu J.T.,Zhu Z.N.,and Qi J.M.,2017,Construction of molecular fingerprinting map for 88 accessions of Cucurbita by SRAP markers,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),2(18):225-232(陶爱芬,魏嘉俊,刘星,徐建堂,朱忠南,祁建民,2017,应用SRAP标记绘制88份南瓜属种质资源DNA指纹图谱,植物遗传资源学报,2(18):225-232)
Wang L.H.,Zheng Y.H.,and Tang G.G.,2010,ISSR analysis of genetic diversity of eight populations Malus baccata,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),30(7):1337-1343(王雷宏,郑玉红,汤庚国,2010,8个山荆子居群多样性的ISSR分析,西北植物学报,30(7):1337-1343)
Wang X.,2016,Genetic diversity of Styrax obassia Sieb.et Zucc based on AFLP markers,Thesis for M.S.,Shandong University,Supervisor:Xing S.Y.,pp.29-31(王萱,2016,玉铃花遗传多样性的AFLP分析,硕士学位论文,山东农业大学,导师:邢世岩,pp.29-31)
Xia F.G.,Huang J.X.,Ji B.J.,Zhan F.Y.,Xie P.P.,Deng B.Z.,Lin H.,and Zheng J.G.,2017,Genetic diversity analysis and DNA fingerprint construction of Coixlacryma-jobi germplasm resources by SRAP marker,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),3(18):413-420(夏法刚,黄金星,季彪俊,詹福杨,谢萍萍,邓邦柱,林宏,郑金贵,2017,基于SRAP标记的薏苡种质资源遗传多样性及DNA指纹图谱构建,植物遗传资源学报,3(18):413-420)
Xue Z.Z.,Yang Y.H.,Li H.S.,Zhang G.X.,and Liu S.J.,2017,Genetic diversity analysis of 58 germplasm resources of Helianthus tuberosus by SRAP marker,Beifang Yuanyi(Northern Horticulture),(21):31-36(薛志忠,杨雅华,李海山,张国新,刘淑君,2017,五十八份菊芋种质资源遗传多样性SRAP分析,北方园艺,(21):31-36)
Yan N.,Guo J.Z.,Cao J.L.,and Yan T.,2016,Establishment of fingerprint and analysis fruit mulberry germplasm resources of genetic difference for by SRAP markers,Xibei Linxueyuan Xuebao(Journal of Northwest Forestry University),3(31):103-108(延娜,郭军战,曹佳乐,严婷,2016,果桑种质资源SRAP指纹图谱构建及遗传差异分析,西北林学院学报,3(31):103-108)
Zhang P.,Tie W.Z.,Lan S.K.,and Luo G.X.,2005,Cuttage experiment of Clerodendranthus spicatus,Yaredai Zhiwu Kexue(Subtropical Plant Science),34(1):66-72(张平,铁万祝,兰世宽,罗关兴,2005,肾茶种苗扦插试验,亚热带植物科学,34(1):66-72)
Zhao N.,and Li H.Q.,2009,Population genetic diversity of Rehmannia glutinosa,Henan Kexue(Henan science),27(11):1386-1391(赵楠,李宏庆,2009,地黄居群遗传多样性的ISSR分析,河南科学,27(11):1386-1391)
Zheng Y.Q.,Guo H.L.,Zang G.Z.,and Liu J.X.,2013,Genetic linkage maps of centipedegrass Eremochloa ophiuroides(Munro)Hack based on sequence-related amplified polymorphism and expressed sequence tag-simple sequence repeat markers,Sci.Hortic.,156(7):86-92
Zhou L.,Wang Y.,Wang X.Y.,and Xu Z.H.,2015,Germplasm characteristics and SRAP marker of Qianxinan prefecture tiepishihu(Dendrobium officinale),Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(9):1950-1956(周丽,王苑,王兴益,徐正海,2015,黔西南州铁皮石斛资源特性及SRAP标记,基因组学与应用生物学,34(9):1950-1956)
Gao F.,2007,Germplasm resources evaluation and seeds quality standard research of Schizonepeta tenuifolia Briq.,Thesis for M.S.,China Academy of Chinese Medical Sciences,Supervisors:Chen M.,and Huang L.Q.,pp.25-28(高峰,2007,荆芥种质资源评价与种子质量标准研究,硕士学位论文,中国中医科学院,导师:陈敏,黄璐琦,pp.25-28)
Gao L.X.,Liu N.,Huang B.H.,and Hu X.,2008,Phylogenetic analysis and genetic mapping of Chinese Hedychium using SRAP markers,Sci.Hortic.,117(4):369-377
Li A.X.,Liu Q.C.,Wang Q.M.,Zhang L.M.,Zhai H.,and Liu S.Z.,2010,Con struction of molecular linkage maps using SRAP markers in Sweetpotato,Acta Agronomica Sinica,36(8):1286-1295
Li G.,and Quiros C.F.,2001,Sequence-related amplified poly morphism(SRAP),a new marker system based on a simple PCR reaction:Its application to mapping and gene tagging in Brassica,Theor.Appl.Genet.,103(3):455-461
Liu Z.M.,Liu Z.H.,and Xu T.Y.,2003,Cultivation technologies of Clerodendranthus spicatus,Shizhen Guoyi Guoyao(Lishizhen Medicine and Materia Medica Research),14(8):497-503(刘志民,刘志红,徐同印,2003,猫须草的栽培技术,时珍国医国药,14(8):497-503)
Pan C.M.,Li W.,He H.,Deng W.Q.,Li T.H.,and Xu H.H.,2006,Study on intraspecific genetic diversity in different plant populations of Pogostemon cablin,Zhongguo Zhongyao Zazhi(China Journal of Chinese Materia Medica),31(9):723-726(潘超美,李薇,贺红,邓旺秋,李泰辉,徐鸿华,2006,不同栽培居群广藿香的种内遗传多样性研究,中国中药杂志,31(9):723-726)
Tao A.F.,Wei J.J.,Liu X.,Xu J.T.,Zhu Z.N.,and Qi J.M.,2017,Construction of molecular fingerprinting map for 88 accessions of Cucurbita by SRAP markers,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),2(18):225-232(陶爱芬,魏嘉俊,刘星,徐建堂,朱忠南,祁建民,2017,应用SRAP标记绘制88份南瓜属种质资源DNA指纹图谱,植物遗传资源学报,2(18):225-232)
Wang L.H.,Zheng Y.H.,and Tang G.G.,2010,ISSR analysis of genetic diversity of eight populations Malus baccata,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),30(7):1337-1343(王雷宏,郑玉红,汤庚国,2010,8个山荆子居群多样性的ISSR分析,西北植物学报,30(7):1337-1343)
Wang X.,2016,Genetic diversity of Styrax obassia Sieb.et Zucc based on AFLP markers,Thesis for M.S.,Shandong University,Supervisor:Xing S.Y.,pp.29-31(王萱,2016,玉铃花遗传多样性的AFLP分析,硕士学位论文,山东农业大学,导师:邢世岩,pp.29-31)
Xia F.G.,Huang J.X.,Ji B.J.,Zhan F.Y.,Xie P.P.,Deng B.Z.,Lin H.,and Zheng J.G.,2017,Genetic diversity analysis and DNA fingerprint construction of Coixlacryma-jobi germplasm resources by SRAP marker,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),3(18):413-420(夏法刚,黄金星,季彪俊,詹福杨,谢萍萍,邓邦柱,林宏,郑金贵,2017,基于SRAP标记的薏苡种质资源遗传多样性及DNA指纹图谱构建,植物遗传资源学报,3(18):413-420)
Xue Z.Z.,Yang Y.H.,Li H.S.,Zhang G.X.,and Liu S.J.,2017,Genetic diversity analysis of 58 germplasm resources of Helianthus tuberosus by SRAP marker,Beifang Yuanyi(Northern Horticulture),(21):31-36(薛志忠,杨雅华,李海山,张国新,刘淑君,2017,五十八份菊芋种质资源遗传多样性SRAP分析,北方园艺,(21):31-36)
Yan N.,Guo J.Z.,Cao J.L.,and Yan T.,2016,Establishment of fingerprint and analysis fruit mulberry germplasm resources of genetic difference for by SRAP markers,Xibei Linxueyuan Xuebao(Journal of Northwest Forestry University),3(31):103-108(延娜,郭军战,曹佳乐,严婷,2016,果桑种质资源SRAP指纹图谱构建及遗传差异分析,西北林学院学报,3(31):103-108)
Zhang P.,Tie W.Z.,Lan S.K.,and Luo G.X.,2005,Cuttage experiment of Clerodendranthus spicatus,Yaredai Zhiwu Kexue(Subtropical Plant Science),34(1):66-72(张平,铁万祝,兰世宽,罗关兴,2005,肾茶种苗扦插试验,亚热带植物科学,34(1):66-72)
Zhao N.,and Li H.Q.,2009,Population genetic diversity of Rehmannia glutinosa,Henan Kexue(Henan science),27(11):1386-1391(赵楠,李宏庆,2009,地黄居群遗传多样性的ISSR分析,河南科学,27(11):1386-1391)
Zheng Y.Q.,Guo H.L.,Zang G.Z.,and Liu J.X.,2013,Genetic linkage maps of centipedegrass Eremochloa ophiuroides(Munro)Hack based on sequence-related amplified polymorphism and expressed sequence tag-simple sequence repeat markers,Sci.Hortic.,156(7):86-92
Zhou L.,Wang Y.,Wang X.Y.,and Xu Z.H.,2015,Germplasm characteristics and SRAP marker of Qianxinan prefecture tiepishihu(Dendrobium officinale),Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(9):1950-1956(周丽,王苑,王兴益,徐正海,2015,黔西南州铁皮石斛资源特性及SRAP标记,基因组学与应用生物学,34(9):1950-1956)
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