氨基糖代谢通路影响尖孢镰刀菌古巴专化型厚垣孢子的形成
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摘要
尖孢镰刀菌古巴专化型Fusarium oxysporum f. sp. cubense(FOC)是威胁香蕉生产的重要土传病原真菌,其厚垣孢子可在土壤中存活多年,是香蕉枯萎病的重要侵染源。为解析该病菌厚垣孢子的形成机制,本研究建立了厚垣孢子的诱导形成体系。通过氨基糖添加试验,证明添加N-乙酰葡糖胺可抑制厚垣孢子的形成;通过对该病菌厚垣孢子形成前期(0h)、初期(24h)、中期(48h)和后期(96h)的转录组分析,发现氨基糖代谢通路中有41个基因的表达水平在厚垣孢子形成过程中发生了变化,其中9个基因与几丁质合成相关,32个基因与糖类化合物的合成和代谢及催化转换相关。本研究首次证明了氨基糖代谢通路与尖孢镰刀菌古巴专化型厚垣孢子形成的相关性。
Fusarium oxysporum f. sp. cubense(FOC) is an important soil-borne pathogenic fungus, which leads to a serious threat to the production of banana in the world. Vast amount of chlamydospores produced by the pathogen are able to survive many years in the infected-soil, which become major infectious sources of this disease. To explore the mechanisms of chlamydospore formation in FOC, an induction system was set up. The result was that the chlamydospore formation of FOC was inhibited by the supplement of N-acetylglucosamine in the induction system. Transcriptome analyses of four chlamydospore formation stages(mycelial stage, 0 h;initial stage, 24 h; middle stage, 48 h; later stage, 96 h) revealed that there were 41 genes varying their expression levels in amino sugar metabolism pathway. Among which, nine genes were related to chitin synthesis and 32 genes were related to synthesis, metabolism and catalytic conversion of carbohydrates. This study confirmed that amino sugar metabolism pathway was involved in chlamydospore formation of FOC.
Fusarium oxysporum f. sp. cubense(FOC) is an important soil-borne pathogenic fungus, which leads to a serious threat to the production of banana in the world. Vast amount of chlamydospores produced by the pathogen are able to survive many years in the infected-soil, which become major infectious sources of this disease. To explore the mechanisms of chlamydospore formation in FOC, an induction system was set up. The result was that the chlamydospore formation of FOC was inhibited by the supplement of N-acetylglucosamine in the induction system. Transcriptome analyses of four chlamydospore formation stages(mycelial stage, 0 h;initial stage, 24 h; middle stage, 48 h; later stage, 96 h) revealed that there were 41 genes varying their expression levels in amino sugar metabolism pathway. Among which, nine genes were related to chitin synthesis and 32 genes were related to synthesis, metabolism and catalytic conversion of carbohydrates. This study confirmed that amino sugar metabolism pathway was involved in chlamydospore formation of FOC.
引文
Alonso-Monge R,Navarro-Garcia F,Roman E,Negredo AI,Eisman B,Nombela C,Pla J,2003.The Hog1mitogen-activated protein kinase is essential in the oxidative stress response and chlamydospore formation in Candida albicans.Eukaryotic Cell,2(2):351-361
B?ttcher B,P?llath C,Staib P,Hube B,Brunke S,2016.Candida species rewired hyphae developmental programs for chlamydospore formation.Frontiers in Microbiology,7:1697
Couteaudier Y,Alabouvette C,1990.Survival and inoculum potential of conidia and chlamydospores of Fusarium oxysporum f.sp.lini in soil.Canadian Journal of Microbiology,36:551-556
Ding Z,Li M,Sun F,Xi P,Sun L,Zhang L,Jiang Z,2015.Mitogen-activated protein kinases are associated with the regulation of physiological traits and virulence in Fusarium oxysporum f.sp.cubense.PLoS One,10:e0122634
Ding ZJ,Qi YX,Zeng FY,Peng J,Xie YX,Zhang X,2018.An transcription factor FoSwi6 regulates physiology traits and virulence in Fusarium oxysporum f.sp.cubense.Acta Phytopathologica Sinica,48(5):601-610(in Chinese)
Ding Z,Yang L,Wang G,Guo L,Liu L,Wang J,Huang J,2018.Fusaric acid is a virulence factor of Fusarium oxysporum f.sp.cubense on banana plantlets.Tropical Plant Pathology,43:297-305
Dong ZY,Wang Q,Qin SW,Wang ZZ,2010.Comparison of cell wall degrading enzymes produced by Fusarium oxysporum f.sp.cubense race 1 and race 4.Acta Phytopathologica Sinica,40(5):463-468(in Chinese)
Griffin GJ,1976.Roles of low pH,carbon and inorganic nitrogen source use in chlamydospore formation by Fusarium solani.Canadian Journal of Microbiology,22(9):1381-1389
Guo L,Yang L,Liang C,Wang J,Liu L,Huang J,2016.The G-protein subunits FGA2 and FGB1 play distinct roles in development and pathogenicity in the banana fungal pathogen Fusarium oxysporum f.sp.cubense.Physiological and Molecular Plant Pathology,93:29-38
Hwang SC,Ko WH,2004.Cavendish banana cultivars resistant to Fusarium wilt acquired through somaclonal variation in Taiwan.Plant Disease,88(6):580-588
Ingle S,Kodgire S,Shiradhone A,Patil R,Zore G,2017.Chlamydospore specific proteins of Candida albicans.Data,2(3):26
Katan T,Shlevin E,Katan J,1997.Sporulation of Fusarium oxysporum f.sp.lycopersici on stem surfaces of tomato plants and aerial dissemination of inoculum.Phytopathology,87(7):712-719
Li C,Zuo C,Deng G,Kuang R,Yang Q,Hu C,Sheng O,Zhang S,Ma L,Wei Y,Yang J,Liu S,Biswas MK,Viljoen A,Yi G,2013.Contamination of bananas with beauvericin and fusaric acid produced by Fusarium oxysporum f.sp.cubense.PLoS One,8:e70226
Li C,Li YJ,Yu L,Huang BZ,2010.Ingredient analysis and bioassay of the toxin of Fusarium oxysporum f.sp.cubense.Journal of Fruit Science,27(6):969-974(in Chinese)
Li MH,Xi PG,Jiang ZD,Qi PK,2007.Race identification of Fusarium oxysporum f.sp.cubense,the causal agent of banana Fusarium wilt in Guangdong province.Journal of South China Agricultural University,28(2):38-41(in Chinese)
Li MH,Xie XL,Lin XF,Shi JX,Ding ZJ,Ling JF,Xi PG,Zhou JN,Leng Y,Zhong S,Jiang ZD,2014.Functional characterization of the gene FoOCH1 encoding a putative alpha-1,6-mannosyltransferase in Fusarium oxysporum f.sp.cubense.Fungal Genetics and Biology,65:1-13
Mandal DN,Chaudhuri SJIP,2013.Induction of chlamydospore in Fusarium moniliforme.Indian Phytopathology,125(2):794-800
Melo NR,Moran GP,Warrilow AG,Dudley E,Smith SN,Sullivan DJ,Lamb DC,Kelly DE,Coleman DC,Kelly SL,2008.CYP56(Dit2p)in Candida albicans:characterization and investigation of its role in growth and antifungal drug susceptibility.Antimicrobial Agents Chemotherapy,52(10):3718-3724
Mitchell AP,1994.Control of meiotic gene expression in Saccharomyces cerevisiae.Microbiological Reviews,58(1):56-70
Nobile CJ,Bruno VM,Richard ML,Davis DA,Mitchell AP,2003.Genetic control of chlamydospore formation in Candida albicans.Microbiology,149(2):3629-3637
Ohara T,Tsuge T,2004.FoSTUA,encoding a basic helix-loop-helix protein,differentially regulates development of three kinds of asexual spores,macroconidia,microconidia,and chlamydospores,in the fungal plant pathogen Fusarium oxysporum.Eukaryotic Cell,3(6):1412-1422
Palige K,Linde J,Martin R,Bottcher B,Citiulo F,Sullivan DJ,Weber J,Staib C,Rupp S,Hube B,Morschhauser J,Staib P,2013.Global transcriptome sequencing identifies chlamydospore specific markers in Candida albicans and Candida dubliniensis.PLoS One,8:e61940
Qureshi AA,Page OT,1970.Observations on chlamydospore production by Fusarium in a two-salt solution.Canadian Journal of Microbiology,16(1):29-32
Rekah Y,Shtienberg D,Katan J,2000.Disease development following infection of tomato and basil foliage by airborne conidia of the soilborne pathogens Fusarium oxysporum f.sp.radicis-lycopersici and F.oxysporum f.sp.basilici.Phytopathology,90(2):1322-1329
Rowe RC,Farely JD,Coplin DC,1977.Airborne spore dispersal and recolonization of steamed soil by Fusarium oxysporum in greenhouses.Phytopathology,67:1513-1517
Schneider EF,Barran LR,Wood PJ,Siddiqui IR,1977.Cell wall of Fusarium sulphureum.II.Chemical composition of the conidial and chlamydospore walls.Canadian Journal of Microbiology,23(6):763-769
Sonneborn A,Bockmuhl DP,Ernst JF,1999.Chlamydospore formation in Candida albicans requires the Efg1p morphogenetic regulator.Infection and Immunity,67(10):5514-5517
Stevenson IL,Becker SA,1972.The fine structure and development of chlamydospores of Fusarium oxysporum.Canadian Journal of Microbiology,18(7):997-1002
Xu LB,Zhang XY,Li HP,Cheng B,Huang BZ,Cheng WX,Feng Y,Xiao WQ,Zhou DB,Gan DQ,2017.The breeding of new banana varieties‘Nan Tian Huang’for resistance to Fusarium wilt.Chinese Journal of Tropical Crops,38(6):998-1004(in Chinese)
Yang XY,Li M,Zhang L,Pang L,Sun Q,Jiang XL,2015.Transcriptome analysis of Trichoderma harzianum Th-33in chlamydospore formation.Chinese Journal of Biological Control,31(1):85-95(in Chinese)
丁兆建,漆艳香,曾凡云,彭军,谢艺贤,张欣,2018.转录因子FoSwi6调控香蕉枯萎病菌的生理特性和致病性.植物病理学报,48(5):601-610
董章勇,王琪,秦世雯,王振中,2010.香蕉枯萎病菌1号和4号生理小种细胞壁降解酶的比较.植物病理学报,40(5):463-468
李赤,黎永坚,于莉,黄秉智,2010.香蕉枯萎病菌毒素的成分分析及其生物测定.果树学报,27(6):969-974
李敏慧,习平根,姜子德,戚佩坤,2007.广东香蕉枯萎病菌生理小种的鉴定.华南农业大学学报,28(2):38-41
许林兵,张锡炎,李华平,陈彪,黄秉智,陈维信,冯岩,肖维强,周登博,甘东泉,2017.抗枯萎病香蕉新品种‘南天黄’选育.热带作物学报,38(6):998-1004
杨晓燕,李梅,张林,庞莉,孙青,蒋细良,2015.哈茨木霉Th-33厚垣孢子形成过程的转录组变化分析.中国生物防治学报,31(1):85-95
B?ttcher B,P?llath C,Staib P,Hube B,Brunke S,2016.Candida species rewired hyphae developmental programs for chlamydospore formation.Frontiers in Microbiology,7:1697
Couteaudier Y,Alabouvette C,1990.Survival and inoculum potential of conidia and chlamydospores of Fusarium oxysporum f.sp.lini in soil.Canadian Journal of Microbiology,36:551-556
Ding Z,Li M,Sun F,Xi P,Sun L,Zhang L,Jiang Z,2015.Mitogen-activated protein kinases are associated with the regulation of physiological traits and virulence in Fusarium oxysporum f.sp.cubense.PLoS One,10:e0122634
Ding ZJ,Qi YX,Zeng FY,Peng J,Xie YX,Zhang X,2018.An transcription factor FoSwi6 regulates physiology traits and virulence in Fusarium oxysporum f.sp.cubense.Acta Phytopathologica Sinica,48(5):601-610(in Chinese)
Ding Z,Yang L,Wang G,Guo L,Liu L,Wang J,Huang J,2018.Fusaric acid is a virulence factor of Fusarium oxysporum f.sp.cubense on banana plantlets.Tropical Plant Pathology,43:297-305
Dong ZY,Wang Q,Qin SW,Wang ZZ,2010.Comparison of cell wall degrading enzymes produced by Fusarium oxysporum f.sp.cubense race 1 and race 4.Acta Phytopathologica Sinica,40(5):463-468(in Chinese)
Griffin GJ,1976.Roles of low pH,carbon and inorganic nitrogen source use in chlamydospore formation by Fusarium solani.Canadian Journal of Microbiology,22(9):1381-1389
Guo L,Yang L,Liang C,Wang J,Liu L,Huang J,2016.The G-protein subunits FGA2 and FGB1 play distinct roles in development and pathogenicity in the banana fungal pathogen Fusarium oxysporum f.sp.cubense.Physiological and Molecular Plant Pathology,93:29-38
Hwang SC,Ko WH,2004.Cavendish banana cultivars resistant to Fusarium wilt acquired through somaclonal variation in Taiwan.Plant Disease,88(6):580-588
Ingle S,Kodgire S,Shiradhone A,Patil R,Zore G,2017.Chlamydospore specific proteins of Candida albicans.Data,2(3):26
Katan T,Shlevin E,Katan J,1997.Sporulation of Fusarium oxysporum f.sp.lycopersici on stem surfaces of tomato plants and aerial dissemination of inoculum.Phytopathology,87(7):712-719
Li C,Zuo C,Deng G,Kuang R,Yang Q,Hu C,Sheng O,Zhang S,Ma L,Wei Y,Yang J,Liu S,Biswas MK,Viljoen A,Yi G,2013.Contamination of bananas with beauvericin and fusaric acid produced by Fusarium oxysporum f.sp.cubense.PLoS One,8:e70226
Li C,Li YJ,Yu L,Huang BZ,2010.Ingredient analysis and bioassay of the toxin of Fusarium oxysporum f.sp.cubense.Journal of Fruit Science,27(6):969-974(in Chinese)
Li MH,Xi PG,Jiang ZD,Qi PK,2007.Race identification of Fusarium oxysporum f.sp.cubense,the causal agent of banana Fusarium wilt in Guangdong province.Journal of South China Agricultural University,28(2):38-41(in Chinese)
Li MH,Xie XL,Lin XF,Shi JX,Ding ZJ,Ling JF,Xi PG,Zhou JN,Leng Y,Zhong S,Jiang ZD,2014.Functional characterization of the gene FoOCH1 encoding a putative alpha-1,6-mannosyltransferase in Fusarium oxysporum f.sp.cubense.Fungal Genetics and Biology,65:1-13
Mandal DN,Chaudhuri SJIP,2013.Induction of chlamydospore in Fusarium moniliforme.Indian Phytopathology,125(2):794-800
Melo NR,Moran GP,Warrilow AG,Dudley E,Smith SN,Sullivan DJ,Lamb DC,Kelly DE,Coleman DC,Kelly SL,2008.CYP56(Dit2p)in Candida albicans:characterization and investigation of its role in growth and antifungal drug susceptibility.Antimicrobial Agents Chemotherapy,52(10):3718-3724
Mitchell AP,1994.Control of meiotic gene expression in Saccharomyces cerevisiae.Microbiological Reviews,58(1):56-70
Nobile CJ,Bruno VM,Richard ML,Davis DA,Mitchell AP,2003.Genetic control of chlamydospore formation in Candida albicans.Microbiology,149(2):3629-3637
Ohara T,Tsuge T,2004.FoSTUA,encoding a basic helix-loop-helix protein,differentially regulates development of three kinds of asexual spores,macroconidia,microconidia,and chlamydospores,in the fungal plant pathogen Fusarium oxysporum.Eukaryotic Cell,3(6):1412-1422
Palige K,Linde J,Martin R,Bottcher B,Citiulo F,Sullivan DJ,Weber J,Staib C,Rupp S,Hube B,Morschhauser J,Staib P,2013.Global transcriptome sequencing identifies chlamydospore specific markers in Candida albicans and Candida dubliniensis.PLoS One,8:e61940
Qureshi AA,Page OT,1970.Observations on chlamydospore production by Fusarium in a two-salt solution.Canadian Journal of Microbiology,16(1):29-32
Rekah Y,Shtienberg D,Katan J,2000.Disease development following infection of tomato and basil foliage by airborne conidia of the soilborne pathogens Fusarium oxysporum f.sp.radicis-lycopersici and F.oxysporum f.sp.basilici.Phytopathology,90(2):1322-1329
Rowe RC,Farely JD,Coplin DC,1977.Airborne spore dispersal and recolonization of steamed soil by Fusarium oxysporum in greenhouses.Phytopathology,67:1513-1517
Schneider EF,Barran LR,Wood PJ,Siddiqui IR,1977.Cell wall of Fusarium sulphureum.II.Chemical composition of the conidial and chlamydospore walls.Canadian Journal of Microbiology,23(6):763-769
Sonneborn A,Bockmuhl DP,Ernst JF,1999.Chlamydospore formation in Candida albicans requires the Efg1p morphogenetic regulator.Infection and Immunity,67(10):5514-5517
Stevenson IL,Becker SA,1972.The fine structure and development of chlamydospores of Fusarium oxysporum.Canadian Journal of Microbiology,18(7):997-1002
Xu LB,Zhang XY,Li HP,Cheng B,Huang BZ,Cheng WX,Feng Y,Xiao WQ,Zhou DB,Gan DQ,2017.The breeding of new banana varieties‘Nan Tian Huang’for resistance to Fusarium wilt.Chinese Journal of Tropical Crops,38(6):998-1004(in Chinese)
Yang XY,Li M,Zhang L,Pang L,Sun Q,Jiang XL,2015.Transcriptome analysis of Trichoderma harzianum Th-33in chlamydospore formation.Chinese Journal of Biological Control,31(1):85-95(in Chinese)
丁兆建,漆艳香,曾凡云,彭军,谢艺贤,张欣,2018.转录因子FoSwi6调控香蕉枯萎病菌的生理特性和致病性.植物病理学报,48(5):601-610
董章勇,王琪,秦世雯,王振中,2010.香蕉枯萎病菌1号和4号生理小种细胞壁降解酶的比较.植物病理学报,40(5):463-468
李赤,黎永坚,于莉,黄秉智,2010.香蕉枯萎病菌毒素的成分分析及其生物测定.果树学报,27(6):969-974
李敏慧,习平根,姜子德,戚佩坤,2007.广东香蕉枯萎病菌生理小种的鉴定.华南农业大学学报,28(2):38-41
许林兵,张锡炎,李华平,陈彪,黄秉智,陈维信,冯岩,肖维强,周登博,甘东泉,2017.抗枯萎病香蕉新品种‘南天黄’选育.热带作物学报,38(6):998-1004
杨晓燕,李梅,张林,庞莉,孙青,蒋细良,2015.哈茨木霉Th-33厚垣孢子形成过程的转录组变化分析.中国生物防治学报,31(1):85-95