甘草酸酶催化水解单葡萄糖醛酸甘草次酸的反应溶剂研究
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摘要
甘草酸(GL)是甘草类中药材中的有效成分,将甘草酸转化为单葡萄糖酸甘草次酸(GAMG)可以提高其药物活性并降低生物毒性。离心切取新鲜猪肝脏匀浆中间段溶酶体作为酶催化剂,用胆碱基深共晶溶剂作为反应溶剂进行酶催化水解反应,用紫外-可见分光光度法对反应产物进行分析,比较了两种介质下的反应转化率。
GL is an important medicinal ingredient in liquorices,Directional transformation of GL to GAMG can improve its drug activity and reduce its toxicity.The lysosome was separated from the pig liver homogenization by centrifuge and used as the enzyme catalyst in bile base deep eutectic solvent.The product was scanned by UV-VIS spectroscopy.The conversion rates in two conditions are compared.
GL is an important medicinal ingredient in liquorices,Directional transformation of GL to GAMG can improve its drug activity and reduce its toxicity.The lysosome was separated from the pig liver homogenization by centrifuge and used as the enzyme catalyst in bile base deep eutectic solvent.The product was scanned by UV-VIS spectroscopy.The conversion rates in two conditions are compared.
引文
[1]黄炜.18β-甘草次酸和甘草次酸对人肝癌细胞增殖的抑制和诱导分化作用.中国中医药科技,2002,9(2):93.
[2]于辉,等.甘草的药理作用概述.现代生物医学进展,2006,6(4):77-79.
[3]Ohtake N,Kido A,Kubota K,et al.Apossible involvement of 3-monoglucuronyl-glycyrrhetinic acid,a metabolite of glycyrrhizin(GL),in GL-induced pseudoaldosteronism[J].Life Sciences,2007,80(17):1545-1552.
[4]Mizutani K,Kuramoto T,Tanaka O,et al.Sweetness of glycyrrhetic acid 3-O-beta-D-monoglu-duronide and the related glycosides[J].Biosci Biotechnol Biochem,1994,58(3):554-555.
[5]鱼红闪,吴少杰,金凤燮,等.酶法改变甘草苷糖醛酸基提高其甜度的研究.食品与发酵工业1998,25(3):10-15.
[6]于荣敏.天然药物活性成分的生物合成与生物催化[J].中草药,2006,37(9):1281-1288.
[7]陈金燕,等.含离子液体体系中固定化细胞转化甘草酸生成单葡萄糖醛酸基甘草次酸.生物加工过程,2011,9(5):18-21.
[8]Takashi K.Microbial production of glycyrrhetic acid 3-0-mono-β-D-glucuronide from glycyrrhizin by cryptococcus magnus MG-27.Biosci Biotech Biochem.1994,58:455-458.
[9]王卓,等.极耐热性β-葡萄糖醛酸酶的高效表达和酶学性质及其应用.生物工程学报,2008,24(8):1407-1412.
[10]冯世江,等.葡糖酸苷酶生产菌株的筛选及其酶学特性的研究[J].高校化学工程学报,2007,21(6):977-982.
[11]吴少杰,等.甘草皂苷生物转化的研究[J].中草药,2003,34(6):516-518.
[12]Kanaoka M,et al.Synthesis and separation of 18β-glycyrrhetyl monoglucuronide from serum of a patient with glycyrrhizin-induced pseudo-aldosteronism.Chem Pharm Bull 34:4978-4983,1986.
[13]Akao T,et al.Hydrolysis of glycyrrhizin to18 beta-glycyrrhetyl monoglucuronide by lysosomal beta-D-glucuronidase of animal livers[J].Biochem Pharmacol,1991,41(6-7):1025-1029.
[14]邹树平,等.水/有机溶剂体系中产紫青霉全细胞催化合成单葡糖醛酸基甘草次酸[J].化工学报,2009,60(8):2040-2045.
[2]于辉,等.甘草的药理作用概述.现代生物医学进展,2006,6(4):77-79.
[3]Ohtake N,Kido A,Kubota K,et al.Apossible involvement of 3-monoglucuronyl-glycyrrhetinic acid,a metabolite of glycyrrhizin(GL),in GL-induced pseudoaldosteronism[J].Life Sciences,2007,80(17):1545-1552.
[4]Mizutani K,Kuramoto T,Tanaka O,et al.Sweetness of glycyrrhetic acid 3-O-beta-D-monoglu-duronide and the related glycosides[J].Biosci Biotechnol Biochem,1994,58(3):554-555.
[5]鱼红闪,吴少杰,金凤燮,等.酶法改变甘草苷糖醛酸基提高其甜度的研究.食品与发酵工业1998,25(3):10-15.
[6]于荣敏.天然药物活性成分的生物合成与生物催化[J].中草药,2006,37(9):1281-1288.
[7]陈金燕,等.含离子液体体系中固定化细胞转化甘草酸生成单葡萄糖醛酸基甘草次酸.生物加工过程,2011,9(5):18-21.
[8]Takashi K.Microbial production of glycyrrhetic acid 3-0-mono-β-D-glucuronide from glycyrrhizin by cryptococcus magnus MG-27.Biosci Biotech Biochem.1994,58:455-458.
[9]王卓,等.极耐热性β-葡萄糖醛酸酶的高效表达和酶学性质及其应用.生物工程学报,2008,24(8):1407-1412.
[10]冯世江,等.葡糖酸苷酶生产菌株的筛选及其酶学特性的研究[J].高校化学工程学报,2007,21(6):977-982.
[11]吴少杰,等.甘草皂苷生物转化的研究[J].中草药,2003,34(6):516-518.
[12]Kanaoka M,et al.Synthesis and separation of 18β-glycyrrhetyl monoglucuronide from serum of a patient with glycyrrhizin-induced pseudo-aldosteronism.Chem Pharm Bull 34:4978-4983,1986.
[13]Akao T,et al.Hydrolysis of glycyrrhizin to18 beta-glycyrrhetyl monoglucuronide by lysosomal beta-D-glucuronidase of animal livers[J].Biochem Pharmacol,1991,41(6-7):1025-1029.
[14]邹树平,等.水/有机溶剂体系中产紫青霉全细胞催化合成单葡糖醛酸基甘草次酸[J].化工学报,2009,60(8):2040-2045.