有色大麦种子种皮颜色与种子活力相关性分析
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摘要
为研究有色大麦种质物理指标与种子活力的关系,采用发芽试验和计算机图像识别相结合的方法,通过测定44份有色大麦种子萌发期生理指标和种子物理指标,用相关性分析、主成分分析和逐步回归分析方法,研究有色大麦种子物理指标与种子活力之间的关系。结果表明:种子粒长、粒宽、粒厚和千粒重这些物理指标与有色大麦种子活力关系不明显,种皮颜色Y值(黄色)与有色大麦种子活力呈正相关关系,其余各颜色指标均与有色大麦种子活力呈负相关关系;逐步回归分析表明,种皮颜色对有色大麦种子活力的影响大于种子粒长、粒宽和粒厚,影响有色大麦种子活力的主要颜色指标是H(色相)值和a(从洋红色至绿色的范围)值,且成负相关;此外,IOD(光密度)值与粒长和粒宽达极显著正相关关系。
To study the relationship between the physical characteristics and seed vigor of barley germplasm,a total of 44 colored barley germplasms were taken as study object.Combination of germination test and computer image recognition was used to determine the relationship between seed physical characteristics and seed vigor of colored barley by measuring the germination characteristics and seed physiological characteristics of barley seeds.Correlation analysis,principal component analysis and stepwise regression analysis were conducted.The results showed that:The germplasm vigor of colored barley was positively correlated with the Y(Yellow) value of seed coat color,and the other color characteristics were negatively correlated with the germplasm of colored barley.Stepwise regression analysis showed that the effect of seed coat color on the vigor of colored barley germplasm was greater than that of seed physical characteristics,grain length and grain thickness.The seed physical characteristics showed that the grain length,grain width,grain thickness and 1 000-grain weight were not significantly related to the seed vigor of colored barley,and the main color characteristics affecting the vigor of colored barley germplasm are H(hues) value and a(range from magenta to green),and are negatively correlated;In addition,the Integrated optical density(IOD) value is extremely positively correlated with the grain length and the grain width.
To study the relationship between the physical characteristics and seed vigor of barley germplasm,a total of 44 colored barley germplasms were taken as study object.Combination of germination test and computer image recognition was used to determine the relationship between seed physical characteristics and seed vigor of colored barley by measuring the germination characteristics and seed physiological characteristics of barley seeds.Correlation analysis,principal component analysis and stepwise regression analysis were conducted.The results showed that:The germplasm vigor of colored barley was positively correlated with the Y(Yellow) value of seed coat color,and the other color characteristics were negatively correlated with the germplasm of colored barley.Stepwise regression analysis showed that the effect of seed coat color on the vigor of colored barley germplasm was greater than that of seed physical characteristics,grain length and grain thickness.The seed physical characteristics showed that the grain length,grain width,grain thickness and 1 000-grain weight were not significantly related to the seed vigor of colored barley,and the main color characteristics affecting the vigor of colored barley germplasm are H(hues) value and a(range from magenta to green),and are negatively correlated;In addition,the Integrated optical density(IOD) value is extremely positively correlated with the grain length and the grain width.
引文
[1] 王建林.西藏高原作物栽培学[M].北京:中国农业出版社,2012Wang J L.Tibet Plateau Crop Cultivation[M].Beijing:China Agriculture Press,2012 (in Chinese)
[2] Nils S G J,Muehlbauer.The Barley Genome[M].Switzerland:Springer International Publishing AG,2018
[3] The International Barley Genome Sequencing Consortium.A physical,genetic and functional sequence assembly of the barley genome[J].Nature,2012,491(7426):711-716
[4] 龚凌霄.青稞全谷物及其防治代谢综合征的作用研究[D].杭州:浙江大学,2013Gong L X.Studies on whole grain of Tibetan hull-less barley and its effect on metabolic syndrome[D].Hangzhou:Zhejiang University,2013 (in Chinese)
[5] 徐菲,党斌,杨希娟,吴昆仑,迟德钊.不同青稞品种的营养品质评价[J].麦类作物学报,2016,36(9):1249-1257Xu F,Dang B,Yang X J,Wu K L,Chi D Z.Evaluation of nutritional quality of different hulless barleys[J].Journal of Triticeae Crops,2016,36(9):1249-1257 (in Chinese)
[6] 刘清斌,刘达玉,冯治平,黄单.青稞酒及其生产技术[J].食品研究与开发,2002,23 (4):45-47Liu Q B,Liu D Y,Feng Z P,Huang D.Green barley wine and its production technology[J].Food Research and Development,2002,23 (4):45-47 (in Chinese)
[7] 扎桑拉姆.浅析青稞原料主要营养成分与青稞产业的发[J].西藏科技,2006,(10):6-7,55Zhasanglamu.Analysis on the main nutrients of green barley raw materials and the development of barley industry[J].Tibet Science and Technology,2006,(10):6-7,55 (in Chinese)
[8] Ostman E,Rossi E,Larsson H,Brighentib F,Bj?rcka I.Glucose and insulin responses in healthy men to barley bread with different levels of (1 to 3;1 to 4)-β-glucans;predictions using fluidity measurements of in vitro enzyme digests[J].Journal of Cereal Science,2006,43(2):230-235
[9] Mullick D B,Fairs D G,Brink V C,Acheson R M.Anthocyanins and anthocyanidins of the barley pericarp and aleurone tissues[J].Canadian Journal of Plant Science,1958,38(4):445-456
[10] Abdel-Aal E S M,Young J C,Rabalski I.Anthocyanin composition in black,blue,pink,purple and red cereal grains[J].Journal of Agricultural and Food Chemistry,2006,54(13):4696-4704
[11] Seeram N P,Momin R A,Nair M G,Bourquinb L D.Cyclooxygenase inhibitory and antioxidant cyanidin glycosides in cherries and berries[J].Phytomedicine,2001,8(5):362-9
[12] Katsuzaki H,Hibasami H,Ohwaki S,Ishikawa K,Imai K,Date K,Kimura Y,Komiya T.Cyanidin-3-O-β-D-glucoside isolated from skin of black Glycine max and other anthocyanins isolated from skin of red grape induce apoptosis in human lymphoid leukemia Molt 4B cells[J].Oncology Reports,2003,10(2):297-300
[13] Hyun J W,Chung H S.Cyanidin and malvidin from Oryza sativa cv Heugjinju-byeo mediate cytotoxicity against human monocytic leukemia cells by arrest of G(2)/M phase and induction of apoptosis[J].Journal of Agricultural and Food Chemistry,2004,52(8):2213-2217
[14] Fimognari C,Berti F,Nusse M,Forti G C,Hrelia P,Hrelia P.In vitro antitumor activity of cyanidin-3-O-β-glucopyranoside[J].Chemotheraphy,2005,51(6):332-335
[15] Chen P K,Chu S C,Chiou H L,Chu S C,Chiou H L,Kuo W H,Chiang C L,Hsieh Y S.Mulberry anthocyanins,cyanidin-3-rutinoside and cyanidin-3-glucoside,exhibited and inhibitory effect on the migration and invasion of a human lung cancer cell line[J].Cancer Letters,2006,235(2):248-259
[16] Shen M,Li K,Jing H,Zheng L.In vivo therapeutic effect of vaccinium Meridionale Swartz in ischemia-reperfusion induced male albino rats[J].Journal of Food Science,2018,83(1):221-228
[17] Fattore M,Montesano D,Pagano E,Teta R,Borrelli F,Mangoni A,Seccia S,Albrizio S.Carotenoid and flavonoid profile and antioxidant activity in “Pomodorino Vesuviano” tomatoes[J].Journal of Food Composition and Analysis,2016,53:61-68
[18] Nascimento K,Sattler J A,Macedo L F L.Muradian L B A.Phenolic compounds,antioxidant capacity and physicochemical properties of Brazilian Apis mellifera honeys[J].LWT-Food science and technology,2018,91:85-94
[19] Dao P T,Ram H H.Genetics of seed longevity in soybean[J].Soybean Science,1999,18(4):312-317
[20] Atak M,Kaya M,Kaya G,Kaya M,Khalid A,Khawar K M.Dark green colored seeds increase the seed vigor and germination ability in dry green pea (Pisum sativum L)[J].Pakistan Journal of Botany,2008,40(6):2345-2354
[21] 李贺勤,张文健,江绪文,林琪.种子大小和种皮颜色对甘蓝种子活力的影响[J].种子,2013,32(10):46-49Li H Q,Zhang W J,Jiang X W,Lin Q.Effects of seed size and seed coat colour on seed vigor of Brassica oleracea L var capitata L[J].Seed,2013,32(10):46-49 (in Chinese)
[22] 齐雪峰,孙群,杨力钢,王建华,孙宝启.种皮颜色对乌拉尔甘草种子质量的影响[J].种子,2007,26(7):31-33Qi X F,Sun Q,Yang L G,Wang J H,Sun B Q,Effec to seed-coat color on seed quality of Glycyrrhiza ura lensis Fisc[J].Seed,2007,26(7):31-33 (in Chinese)
[23] 邹吉祥,金华,朴仁哲,刘磊,姜国斌,王颖,王艳丽.不同颜色羊草种子对其发芽率及愈伤组织诱导的影响研究[J].安徽农业科学,2012,40(3):1501-1503Zou J X,Jin H,Pu R Z,Liu L,Jiang G B,Wang Y,Wang Y L.Seed germination and callus induction in leymus chinensis of different seed colors[J].Journal of Anhui Agricultural Sciences,2012,40(3):1501-150 (in Chinese)
[24] Anuradha R,Balamurugan P,Srimathi P,Sumathi S.Influence of seed coat colour variations on seed quality in bengalgram cv CO 4 (Cicer arietinum L)[J].Legume Research,2009,32(2):136-138
[25] 时伟芳,贾佳,冯鹏飞,谢宗铭,杨丽明,王建华,孙群.春小麦种子物理指标与种子活力关系的初步分析[J].中国农业大学学报,2016,21(7):1-12Shi W F,Jia J,Feng P F,Xie Z M,Yang L M,Wang J H,Sun Q.Preliminary analysis on correlations between seed vigor and physical traits of spring wheat[J].Journal of China Agricultural University,2016,21(7):1-12 (in Chinese)
[26] 贾佳,王建华,谢宗铭,杨丽明,孙宝启,孙群.小麦种子物理参数和萌发阶段抗旱性关系的研究[J].麦类作物学报,2014,34(12):1661-1666Jia J,Wang J H,Xie Z M,Yang L M,Sun B Q,Sun Q.Relationship between physical parameters and drought resistance of wheat seed at germination stage[J].Journal of Triticeae Crops,2014,34(12):1661-1666 (in Chinese)
[27] Filho F,Carvalho N,Moro F.Relationship between physical,morphological,and physiological characteristics of seeds developed at different positions of the ear of two maize (Zea mayz L) hybrids[J].Seed Science and Technology,2002,30(1):97-106
[28] 胡晋.种子生物学[M].北京:高等教育出版社,2006Hu J.Seed Biology[M].Beijing:Higher education press,2006 (in Chinese)
[29] Abati J,Brzezinski C R,Zucareli C,Foloni J S S,Henning F A.Growth and yield of wheat in response to seed vigor and sowing densities[J].Revista Caatinga,2018,31(4):891-899
[30] 王晶.玉米种子理化性状与种子活力的关系[D].沈阳:沈阳农业大学,2017Wang J.Relationship between physical and chemical properties of maize seeds and seed vigor[D].Shenyang:Shenyang Agricultural University,2017 (in Chinese)
[31] 贾佳,王建华,谢宗铭,杨丽明,孙宝启,孙群.计算机图像识别技术在小麦种子精选中的应用[J].中国农业大学学报,2014,19(5):180-186Jia J,Wang J H,Xie Z M,Yang L M,Sun B Q,Sun Q.Wheat seeds selection based on computer image recognition technique[J].Journal of China Agricultural University,2014,19(5):180-186 (in Chinese)
[32] Rezapour R,Hamdollah K A,Yarnia M,Parisa Z M.Effect of seed size on germination and seed vigor of two soybean (glycin max L) cultivars[J].International Research Journal of Applied and Basic Sciences,2013,4(11):3396-3401
[33] 常晖,程秋香,李吟平,张跃进,张小燕.黄芪种子种皮颜色和大小与种子活力相关性研究[J].种子,2015,34(8):95-97,104Chang H,Chen Q X,Li Y P,Zhang Y J,Zhang X Y.Study on the correlation between seed size,seed coat colour and seed vigor of Astragalus membranaceus (Fisch) Bge Var Mongholicus (Bge) Hsia[J].Seed,2015,34(8):95-97,104 (in Chinese)
[2] Nils S G J,Muehlbauer.The Barley Genome[M].Switzerland:Springer International Publishing AG,2018
[3] The International Barley Genome Sequencing Consortium.A physical,genetic and functional sequence assembly of the barley genome[J].Nature,2012,491(7426):711-716
[4] 龚凌霄.青稞全谷物及其防治代谢综合征的作用研究[D].杭州:浙江大学,2013Gong L X.Studies on whole grain of Tibetan hull-less barley and its effect on metabolic syndrome[D].Hangzhou:Zhejiang University,2013 (in Chinese)
[5] 徐菲,党斌,杨希娟,吴昆仑,迟德钊.不同青稞品种的营养品质评价[J].麦类作物学报,2016,36(9):1249-1257Xu F,Dang B,Yang X J,Wu K L,Chi D Z.Evaluation of nutritional quality of different hulless barleys[J].Journal of Triticeae Crops,2016,36(9):1249-1257 (in Chinese)
[6] 刘清斌,刘达玉,冯治平,黄单.青稞酒及其生产技术[J].食品研究与开发,2002,23 (4):45-47Liu Q B,Liu D Y,Feng Z P,Huang D.Green barley wine and its production technology[J].Food Research and Development,2002,23 (4):45-47 (in Chinese)
[7] 扎桑拉姆.浅析青稞原料主要营养成分与青稞产业的发[J].西藏科技,2006,(10):6-7,55Zhasanglamu.Analysis on the main nutrients of green barley raw materials and the development of barley industry[J].Tibet Science and Technology,2006,(10):6-7,55 (in Chinese)
[8] Ostman E,Rossi E,Larsson H,Brighentib F,Bj?rcka I.Glucose and insulin responses in healthy men to barley bread with different levels of (1 to 3;1 to 4)-β-glucans;predictions using fluidity measurements of in vitro enzyme digests[J].Journal of Cereal Science,2006,43(2):230-235
[9] Mullick D B,Fairs D G,Brink V C,Acheson R M.Anthocyanins and anthocyanidins of the barley pericarp and aleurone tissues[J].Canadian Journal of Plant Science,1958,38(4):445-456
[10] Abdel-Aal E S M,Young J C,Rabalski I.Anthocyanin composition in black,blue,pink,purple and red cereal grains[J].Journal of Agricultural and Food Chemistry,2006,54(13):4696-4704
[11] Seeram N P,Momin R A,Nair M G,Bourquinb L D.Cyclooxygenase inhibitory and antioxidant cyanidin glycosides in cherries and berries[J].Phytomedicine,2001,8(5):362-9
[12] Katsuzaki H,Hibasami H,Ohwaki S,Ishikawa K,Imai K,Date K,Kimura Y,Komiya T.Cyanidin-3-O-β-D-glucoside isolated from skin of black Glycine max and other anthocyanins isolated from skin of red grape induce apoptosis in human lymphoid leukemia Molt 4B cells[J].Oncology Reports,2003,10(2):297-300
[13] Hyun J W,Chung H S.Cyanidin and malvidin from Oryza sativa cv Heugjinju-byeo mediate cytotoxicity against human monocytic leukemia cells by arrest of G(2)/M phase and induction of apoptosis[J].Journal of Agricultural and Food Chemistry,2004,52(8):2213-2217
[14] Fimognari C,Berti F,Nusse M,Forti G C,Hrelia P,Hrelia P.In vitro antitumor activity of cyanidin-3-O-β-glucopyranoside[J].Chemotheraphy,2005,51(6):332-335
[15] Chen P K,Chu S C,Chiou H L,Chu S C,Chiou H L,Kuo W H,Chiang C L,Hsieh Y S.Mulberry anthocyanins,cyanidin-3-rutinoside and cyanidin-3-glucoside,exhibited and inhibitory effect on the migration and invasion of a human lung cancer cell line[J].Cancer Letters,2006,235(2):248-259
[16] Shen M,Li K,Jing H,Zheng L.In vivo therapeutic effect of vaccinium Meridionale Swartz in ischemia-reperfusion induced male albino rats[J].Journal of Food Science,2018,83(1):221-228
[17] Fattore M,Montesano D,Pagano E,Teta R,Borrelli F,Mangoni A,Seccia S,Albrizio S.Carotenoid and flavonoid profile and antioxidant activity in “Pomodorino Vesuviano” tomatoes[J].Journal of Food Composition and Analysis,2016,53:61-68
[18] Nascimento K,Sattler J A,Macedo L F L.Muradian L B A.Phenolic compounds,antioxidant capacity and physicochemical properties of Brazilian Apis mellifera honeys[J].LWT-Food science and technology,2018,91:85-94
[19] Dao P T,Ram H H.Genetics of seed longevity in soybean[J].Soybean Science,1999,18(4):312-317
[20] Atak M,Kaya M,Kaya G,Kaya M,Khalid A,Khawar K M.Dark green colored seeds increase the seed vigor and germination ability in dry green pea (Pisum sativum L)[J].Pakistan Journal of Botany,2008,40(6):2345-2354
[21] 李贺勤,张文健,江绪文,林琪.种子大小和种皮颜色对甘蓝种子活力的影响[J].种子,2013,32(10):46-49Li H Q,Zhang W J,Jiang X W,Lin Q.Effects of seed size and seed coat colour on seed vigor of Brassica oleracea L var capitata L[J].Seed,2013,32(10):46-49 (in Chinese)
[22] 齐雪峰,孙群,杨力钢,王建华,孙宝启.种皮颜色对乌拉尔甘草种子质量的影响[J].种子,2007,26(7):31-33Qi X F,Sun Q,Yang L G,Wang J H,Sun B Q,Effec to seed-coat color on seed quality of Glycyrrhiza ura lensis Fisc[J].Seed,2007,26(7):31-33 (in Chinese)
[23] 邹吉祥,金华,朴仁哲,刘磊,姜国斌,王颖,王艳丽.不同颜色羊草种子对其发芽率及愈伤组织诱导的影响研究[J].安徽农业科学,2012,40(3):1501-1503Zou J X,Jin H,Pu R Z,Liu L,Jiang G B,Wang Y,Wang Y L.Seed germination and callus induction in leymus chinensis of different seed colors[J].Journal of Anhui Agricultural Sciences,2012,40(3):1501-150 (in Chinese)
[24] Anuradha R,Balamurugan P,Srimathi P,Sumathi S.Influence of seed coat colour variations on seed quality in bengalgram cv CO 4 (Cicer arietinum L)[J].Legume Research,2009,32(2):136-138
[25] 时伟芳,贾佳,冯鹏飞,谢宗铭,杨丽明,王建华,孙群.春小麦种子物理指标与种子活力关系的初步分析[J].中国农业大学学报,2016,21(7):1-12Shi W F,Jia J,Feng P F,Xie Z M,Yang L M,Wang J H,Sun Q.Preliminary analysis on correlations between seed vigor and physical traits of spring wheat[J].Journal of China Agricultural University,2016,21(7):1-12 (in Chinese)
[26] 贾佳,王建华,谢宗铭,杨丽明,孙宝启,孙群.小麦种子物理参数和萌发阶段抗旱性关系的研究[J].麦类作物学报,2014,34(12):1661-1666Jia J,Wang J H,Xie Z M,Yang L M,Sun B Q,Sun Q.Relationship between physical parameters and drought resistance of wheat seed at germination stage[J].Journal of Triticeae Crops,2014,34(12):1661-1666 (in Chinese)
[27] Filho F,Carvalho N,Moro F.Relationship between physical,morphological,and physiological characteristics of seeds developed at different positions of the ear of two maize (Zea mayz L) hybrids[J].Seed Science and Technology,2002,30(1):97-106
[28] 胡晋.种子生物学[M].北京:高等教育出版社,2006Hu J.Seed Biology[M].Beijing:Higher education press,2006 (in Chinese)
[29] Abati J,Brzezinski C R,Zucareli C,Foloni J S S,Henning F A.Growth and yield of wheat in response to seed vigor and sowing densities[J].Revista Caatinga,2018,31(4):891-899
[30] 王晶.玉米种子理化性状与种子活力的关系[D].沈阳:沈阳农业大学,2017Wang J.Relationship between physical and chemical properties of maize seeds and seed vigor[D].Shenyang:Shenyang Agricultural University,2017 (in Chinese)
[31] 贾佳,王建华,谢宗铭,杨丽明,孙宝启,孙群.计算机图像识别技术在小麦种子精选中的应用[J].中国农业大学学报,2014,19(5):180-186Jia J,Wang J H,Xie Z M,Yang L M,Sun B Q,Sun Q.Wheat seeds selection based on computer image recognition technique[J].Journal of China Agricultural University,2014,19(5):180-186 (in Chinese)
[32] Rezapour R,Hamdollah K A,Yarnia M,Parisa Z M.Effect of seed size on germination and seed vigor of two soybean (glycin max L) cultivars[J].International Research Journal of Applied and Basic Sciences,2013,4(11):3396-3401
[33] 常晖,程秋香,李吟平,张跃进,张小燕.黄芪种子种皮颜色和大小与种子活力相关性研究[J].种子,2015,34(8):95-97,104Chang H,Chen Q X,Li Y P,Zhang Y J,Zhang X Y.Study on the correlation between seed size,seed coat colour and seed vigor of Astragalus membranaceus (Fisch) Bge Var Mongholicus (Bge) Hsia[J].Seed,2015,34(8):95-97,104 (in Chinese)