固相萃取与GC-MS/MS联用技术在茶叶农药残留检测中的应用
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摘要
提出一种超声提取,固相萃取净化,结合气相色谱仪-串联质谱仪(GC-MS/MS)检测茶叶的新方法 ,采用空白基质液配标,在0.005μg/mL～0.5μg/mL范围内获良好线性,相关系数在0.9963～0.9992,对空白样品做3个浓度水平的加标回收试验,常见几种农药回收率为81.2%～107.5%,RSD为0.2%～8.9%,(n=5),方法检出限在0.01mg/kg～0.05mg/kg,将此法用于实际样品测试,结果令人满意。
Mixed standard solution was labelled with blank sample matrix,the linear correlation coefficient reached 0.9963-0.9992 in the range of 0.005-0.5μg/mL.The blank tea samples were spiked at the three levels.The recovery of several common pesticide was 81.2%-107.5%.The RSD was 0.2%-8.9%(n=5),the detection limit was 0.01-0.05 mg/kg.The method has been applied to the determination of practical sample.The result is satisfactory.
Mixed standard solution was labelled with blank sample matrix,the linear correlation coefficient reached 0.9963-0.9992 in the range of 0.005-0.5μg/mL.The blank tea samples were spiked at the three levels.The recovery of several common pesticide was 81.2%-107.5%.The RSD was 0.2%-8.9%(n=5),the detection limit was 0.01-0.05 mg/kg.The method has been applied to the determination of practical sample.The result is satisfactory.
引文
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[10]罗逢健,陈宗懋,汤富彬,等.固相萃取和气相色谱质谱法测定茶叶中34种农药残留[J].农药,2010,49(5):363-366.
[11]贾玮,黄峻榕,凌云,等.高效液相色谱串联质谱法同时测定茶叶中290种农药残留组[J].分析测试学报,2013,32(1):9-22.
[12]钟茂生.液相色谱-串联质谱法测定茶叶中24种农药残留[J].分析测试学报,2018,27(1):13-17.
[2]Khan N,Mukhtar H.Cancer and metastasis:prevention and treatment by green tea[J].Cancer Metastasis Rev,2010,29(3):435-445.
[3]Oak M H,Bedoui J E,Schinikerth V B.Antiangiogenic properties of natural polyphenols from red wine and green tea[J].J NutrBiochem,2005,16(1):1-8.
[4]Weinreb O,Mandel S,Amit T,et al.Neurological mechanisms of green tea polyphenols in alzheimer's and parkinson's diseases[J].J NutrBiochem,2004,15(9):506-516.
[5]刘小文,吴国星,高熹.几种新技术在茶叶农药残留分析样品前处理中的应用[J].云南大学学报(自然科学版),2008,30(S1):210-214.
[6]朱雪琴,赵薇,陈坦林,等.改良QuEchers法提取-气相色谱-火焰光度检测同时测定茶叶中5种有机磷农药残留[J].分析测试学报,2018,34(2):176-180.
[7]温裕云,弓振斌,姚剑敏.柱后光化学反应荧光检测高效液相色谱法测定茶叶中的五种菊酯类农药残留[J].分析化学研究报告,2005,33(3):301-304.
[8]刘慧,徐怡,游燕,等.气相色谱-质谱联用法测定茶叶中17种农药残留[J].广州化工,2017,45(18):87-89.
[9]游飞明,吴芳,陆万平,等.固相萃取气相色谱法测定茶叶中多种有机磷农药[J].分析测试技术与仪器,2011,17(1):6-10.
[10]罗逢健,陈宗懋,汤富彬,等.固相萃取和气相色谱质谱法测定茶叶中34种农药残留[J].农药,2010,49(5):363-366.
[11]贾玮,黄峻榕,凌云,等.高效液相色谱串联质谱法同时测定茶叶中290种农药残留组[J].分析测试学报,2013,32(1):9-22.
[12]钟茂生.液相色谱-串联质谱法测定茶叶中24种农药残留[J].分析测试学报,2018,27(1):13-17.