HPLC法同时测定三叶青块根中6个黄酮类成分的含量

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英文篇名：Simultaneous Determination of 6 Flavonoids in the Roots of Tetrastigma hemsleyanum by HPLC 作者：李彩凤 ; 胡欣 ; 金鹏飞 ; 李清 ; 孙淑仃 ; 毕开顺 ; 付宏征 英文作者：LI Caifeng;HU Xin;JIN Pengfei;LI Qing;SUN Shuding;BI Kaishun;FU Hongzheng;College of Pharmacy,Shenyang Pharmaceutical University;State Key Lab of Natural and Biomimetic Drugs,Peking University Health Science Center;Dept.of Pharmaceutical Sciences,Beijing Hospital; 关键词：三叶青 ; 芦丁 ; 异槲皮苷 ; 山柰酚-3-O-芸香糖苷 ; 紫云英苷 ; 槲皮素 ; 山柰酚 ; 含量测定 ; 高效液相色谱法 英文关键词：Tetrastigma hemsleyanum;;Rutin;;Isoquercitrin;;Kaempferol-3-O-rutinoside;;Daunorubicin;;Quercetin;;Kaempferol;;Content determination;;HPLC 中文刊名：中国药房 英文刊名：China Pharmacy 机构：沈阳药科大学药学院;北京大学医学部天然药物及仿生药物国家重点实验室;北京医院药学部; 出版日期：2019-07-15 出版单位：中国药房 年：2019 期：13 基金：国家自然科学基金资助项目(No.81303216) 语种：中文; 页：33-36 页数：4 CN：50-1055/R ISSN：1001-0408 分类号：R284.1;O657.72

摘要

目的:建立同时测定三叶青块根中芦丁、异槲皮苷、山柰酚-3-O-芸香糖苷、紫云英苷、槲皮素和山柰酚含量的方法。方法:采用高效液相色谱(HPLC)法。色谱柱为Alliance SilGreen C18,流动相为0.2%磷酸水溶液-乙腈溶液(梯度洗脱),流速为1.0mL/min,柱温为35℃,检测波长为360 nm,进样量为15μL。结果:芦丁、异槲皮苷、山柰酚-3-O-芸香糖苷、紫云英苷、槲皮素和山柰酚的检测质量浓度线性范围分别为21.77～217.77、12.37～123.75、13.23～132.31、4.63～46.30、5.75～57.50、3.36～33.66μg/mL(r均=0.999 9);检测限分别为0.217 8、0.123 8、0.066 2、0.046 3、0.191 7、0.112 3μg/mL;定量限分别为0.435 6、0.247 5、0.165 4、0.154 3、0.575 0、0.421 2μg/mL;精密度(n=6)、稳定性(24 h,n=7)、重复性(n=6)试验的RSD均≤3.20%;平均加样回收率分别为96.23%、86.88%、97.51%、97.67%、97.50%、87.46%,RSD分别为1.85%、1.90%、1.84%、1.87%、1.25%、2.01%(n=9)。结论:该方法快速、简便,可用于同时测定三叶青块根中芦丁、异槲皮苷、山柰酚-3-O-芸香糖苷、紫云英苷、槲皮素和山柰酚的含量。
        OBJECTIVE:To establish a method for simultaneous determination of rutin,isoquercitrin,kaempferol-3-Orutinoside,daunorubicin,quercetin and kaempferol in the roots of Tetrastigma hemsleyanum. METHODS:HPLC method was adopted. The determination was performed on Alliance SilGreen C18 column with mobile phase consisted of 0.2% phosphoric acid solution-acetonitrile solution(gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was 360 nm and the column temperature was at 35 ℃. The sample size was 15 μL. RESULTS:The linear range of rutin,isoquercitrin,kaempferol-3-O-rutinoside,daunorubicin,quercetin and kaempferol were 21.77-217.77,12.37-123.75,13.23-132.31,4.63-46.30,5.75-57.50,3.36-33.66 μg/mL(all r=0.999 9),respectively. limit of detection of them were 0.217 8,0.123 8,0.066 2,0.046 3,0.191 7,0.112 3 μg/mL,respectively. limit of quantitation of them were 0.435 6,0.247 5,0.165 4,0.154 3,0.575 0,0.421 2 μg/mL,respectively. RSDs of precision(n=6),stability(24 h,n=7) and reproducibility tests(n=6) were lower than 3.20%. The average recoveries of them were 96.23%,86.88%,97.51%,97.67%,97.50%,87.46%,RSDs were 1.85%,1.90%,1.84%,1.87%,1.25%,2.01%(n=9),respectively. CONCLUSIONS:The method is fast and simple,and could be applied for simultaneous determination of rutin,isoquercitrin,kaempferol-3-O-rutinoside,daunorubicin,quercetin and kaempferol in the roots of T. hemsleyanum.

引文

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