表皮细胞条件培养基对真皮干细胞的影响
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摘要
真皮干细胞(SKPs)是一种源自真皮,在毛囊再生中发挥关键作用的多能干细胞.然而,SKPs的体外扩增是一项尚未完全解决的难题.该研究结果显示,表皮细胞条件培养基能显著促进SKPs的增殖,增加SKPs特征性基因Akp2,Sox2,Bmp4,Nestin1,PDGFa,Clstn2,Trps1,Versican的表达水平,并提高SKPs毛囊诱导能力标志性蛋白-碱性磷酸酶的活性.因此,该研究提示表皮细胞分泌的蛋白对于SKPs的增殖和干性的维持有重要作用.
Skin precursor cells(SKPs) are multipotent stem cells derived from the dermis. SKPs are able to induce hair follicle regeneration. It is considered to be seed cells for the thereby of the skin injury. Their application is limited by the instability of their property during culture expansion. In this study, we employed epidermal cell conditioned medium to treat SKPs and found that the proliferation rate of SKPs was increased. In addition, the expression levels of SKPs signature genes Akp2, Sox2, Bmp4, Nestin1, PDGFa, Clstn2, Trps1 and Versican were up-regulated. In addition, the activity of alkaline phosphatase was enhanced significantly which reflect the hair follicle inducing ability of SKPs. Our study indicates that factors released by epidermal cells facilitate the stability of SKPs properties.
Skin precursor cells(SKPs) are multipotent stem cells derived from the dermis. SKPs are able to induce hair follicle regeneration. It is considered to be seed cells for the thereby of the skin injury. Their application is limited by the instability of their property during culture expansion. In this study, we employed epidermal cell conditioned medium to treat SKPs and found that the proliferation rate of SKPs was increased. In addition, the expression levels of SKPs signature genes Akp2, Sox2, Bmp4, Nestin1, PDGFa, Clstn2, Trps1 and Versican were up-regulated. In addition, the activity of alkaline phosphatase was enhanced significantly which reflect the hair follicle inducing ability of SKPs. Our study indicates that factors released by epidermal cells facilitate the stability of SKPs properties.
引文
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[10]Surrao D C,Boon K,Borys B,et al.Large-scale expansion of human skin-derived precursor cells(hSKPs)in stirred suspension bioreactors[J].Biotechnol Bioeng,2016,113(12):2 725-2 738.DOI:10.1002/bit.v113.12.
[11]Lei M,Schumacher L J,Lai Y C,et al.Self-organization process in newborn skin organoid formation inspires strategy to restore hair regeneration of adult cells[J].Proceedings of the National Academy of Sciences of the United States of America,2017,114(34):E7101.DOI:10.1073/pnas.1700475114.
[2]Gabriel V.Hypertrophic Scar[J].Physical Medicine&Rehabilitation Clinics of North America,2011,22(2):301-310.
[3]Esselman P C.Burn rehabilitation:An overview[J].Archives of Physical Medicine&Rehabilitation,2007,88(12):3-6.
[4]Driskell R R,Clavel C,Rendl M,et al.Hair follicle dermal papilla cells at a glance[J].Journal of Cell Science,2011,124(8):1 179-1 182.DOI:10.1242/jcs.082446.
[5]Lavoie J F,Biernaskie J A,Chen Y,et al.Skin-derived precursors differentiate into skeletogenic cell types and contribute to bone repair[J].Stem Cells&Development,2009,18(6):893-906.
[6]Rendl M,Polak L,Fuchs E.BMP signaling in dermal papilla cells is required for their hair follicle-inductive properties[J].Genes&Development,2008,22(4):543-57.
[7]Biernaskie J,Paris M,Morozova O,et al.SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells[J].Cell Stem Cell,2009,5(6):610 -623.DOI:10.1016/j.stem.2009.10.019.
[8]Toma J G,Akhavan M,Fernandes K J L,et al.Isolation of multipotent adult stem cells from the dermis of mammalian skin[J].Nature Cell Biology,2001,3(9):778-784.DOI:10.1038/ncb0901-778.
[9]Agabalyan N A,Borys B S,Sparks H D,et al.Enhanced expansion and sustained inductive function of skin‐derived precursor cells in computer‐controlled stirred suspension bioreactors[J].Stem Cells Translational Medicine,2016,6(2):434-443.
[10]Surrao D C,Boon K,Borys B,et al.Large-scale expansion of human skin-derived precursor cells(hSKPs)in stirred suspension bioreactors[J].Biotechnol Bioeng,2016,113(12):2 725-2 738.DOI:10.1002/bit.v113.12.
[11]Lei M,Schumacher L J,Lai Y C,et al.Self-organization process in newborn skin organoid formation inspires strategy to restore hair regeneration of adult cells[J].Proceedings of the National Academy of Sciences of the United States of America,2017,114(34):E7101.DOI:10.1073/pnas.1700475114.