啤酒花HPLC指纹图谱研究

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英文篇名：Study on fingerprint of Hupulus lupulus by HPLC 作者：董志敏 ; 许月明 ; 闻俊 ; 蒋益萍 ; 秦路平 ; 辛海量 英文作者：DONG Zhimin;XU Yueming;WEN Jun;JIANG Yiping;QIN Luping;XIN Hailiang;College of Life and Environmental Sciences,Shanghai Normal University;School of Pharmacy,Naval Medical University;College of Pharmaceutical Science, Zhejiang Chinese Medical University; 关键词：啤酒花 ; 高效液相色谱 ; 指纹图谱 英文关键词：Hupulus lupulus;;HPLC;;fingerprint 中文刊名：药学实践杂志 英文刊名：Journal of Pharmaceutical Practice 机构：上海师范大学生命与环境科学学院;海军军医大学药学院;浙江中医药大学药学院; 出版日期：2019-01-25 出版单位：药学实践杂志 年：2019 期：01 基金：国家自然科学基金(U1603283) 语种：中文; 页：46-49+58 页数：5 CN：31-1685/R ISSN：1006-0111 分类号：R284.1;O657.72

摘要

目的建立啤酒花HPLC指纹图谱。方法色谱柱:Diamonsil C18柱(250mm×4.6mm,5μm),流动相:乙腈-0.1%磷酸水,梯度洗脱,检测波长358nm,柱压10.2MPa,柱温30℃,进样量10μl,流速1.0ml/min。结果首次建立啤酒花HPLC指纹图谱共有模式,对其19个共有峰进行标定,并对其中的黄腐酚、类葎草酮、葎草酮、加葎草酮、类蛇麻酮、蛇麻酮、加蛇麻酮进行指认。26批药材中有5批药材相似度低于或等于0.9,而该5批均为野生品种。结论该方法高速有效,可准确地对啤酒花栽培种、野生种进行区分,为啤酒花的品质评价研究奠定基础。
        Objective To establish fingerprint of Hupulus lupulus by HPLC.Methods Diamonsil C18column(250mm×4.6mm,5μm)was used.The mobile phase consisted of acetonitrile and 0.1% phosphoric acid,in gradient elution mode,detection wavelength was 358 nm,column pressure at 10.2MPa,column temperature was 30 ℃,injection volume of 10μl and flow rate was 1.0ml/min.Results A common mode of HPLC fingerprint of Hupulus lupulus was established.19 common peaks were calibrated,xanthohumol,cohumulone,humulone,adhumulone,colupulone,lupulone and adlupulone for identification.Among the 26 batches of Hupulus lupulus,5batches of herbs had similarities of less than or equal 0.9,which were all wild varieties.Conclusion The method was fast,effective,accurate and reliably to distinguish the cultivation and wildness Hupulus lupulus,which provided reference for quality control of Hupulus lupulus.

引文

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