蛇足石杉一株盘菌目内生真菌的分类鉴定
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摘要
目的对分离自蛇足石杉的一株盘菌目内生真菌菌株LYS02进行分类鉴定.方法采用形态学、分子生物学和分子系统学方法对蛇足石杉内生真菌菌株LYS02进行了形态学、r DNA ITS的测序和系统发育分析研究.结果菌株LYS02与Pezizales sp. CL309 (KJ407058. 1)有着更近的发育关系.结论菌株LYS02为盘菌目(Pezizales)中的一未知种,该种为微生物资源的开发和利用提供了一种新的菌种资源,可为研究和分析真菌间的系统发育关系提供新的支持材料.
Objective The classification and identification of LYS02,an endophytic fungus strain isolated from Huperzia serrata.Method Morphological characteristics,r DNA ITS region sequencing and phylogenetic analysis of the endophytic fungus strain LYS02 isolated from Huperzia serrata,were studied by using morphological,molecular biological and bioinformatics methods. Results The strains LYS02 has a more recent phylogenetic relationship with Pezales sp. CL309( KJ407058. 1). Conclusion The strain LYS02 was an unknown species in order Pezizales,which provided a new strain for the development and utilization of microbial resources,and new support material for the study and analysis of phylogenetic relationship between fungi.
Objective The classification and identification of LYS02,an endophytic fungus strain isolated from Huperzia serrata.Method Morphological characteristics,r DNA ITS region sequencing and phylogenetic analysis of the endophytic fungus strain LYS02 isolated from Huperzia serrata,were studied by using morphological,molecular biological and bioinformatics methods. Results The strains LYS02 has a more recent phylogenetic relationship with Pezales sp. CL309( KJ407058. 1). Conclusion The strain LYS02 was an unknown species in order Pezizales,which provided a new strain for the development and utilization of microbial resources,and new support material for the study and analysis of phylogenetic relationship between fungi.
引文
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[14]鞠錾,王峻,潘胜利,等.4种石杉科植物中产生石杉碱甲的内生真菌的分离及初步鉴定和HPLC测定石杉碱甲的含量[J].复旦学报(医学版),2009,36(4):445-449.
[15]赖政,汪娣,汪漄,等.具有乙酰胆碱酯酶抑制活性的蛇足石杉内生真菌分子鉴定[J].菌物学报,2014,33(4):858-866.
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[24]王士娟.黄山风景区斑痣盘菌科物种多样性与散斑壳属分子系统学研究[D].合肥:安徽农业大学,2007.
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[27]汪涯,曾庆桂,张志斌,等.蛇足石杉内生真菌分离及其抑制乙酰胆碱酯酶活性研究[J].中国中药杂志,2011,36(6):734-740.
[2]郭良栋.内生真菌研究进展[J].菌物系统,2001,20(1):148-152.
[3]卢占慧.人参内生菌群落多样性及拮抗菌株的抑菌作用研究[D].沈阳:沈阳农业大学,2016.
[4]任慧爽,徐伟芳,王爱印,等.桑树内生细菌多样性及内生拮抗活性菌群的研究[J].西南大学学报(自然科学版),2017,39(1):36-45.
[5]王莉莉,吕会芳,张丽,等.蛇足石杉内生真菌抗乙酰胆碱酯酶活性筛选及分类鉴定[J].中国中药杂志,2012,37(24):3701-3705.
[6]黎万奎,胡之壁.内生菌与天然药物[J].中国天然药物,2005,3(4):193-199.
[7]杨志军,邓毅,曼琼,等.内生菌在天然药物研究中的研究进展[J].中国临床药理学杂志,2018,34(5):593-596.
[8]张磊,万谦宏,高文远.石杉碱甲的研究进展[J].中草药,2005,36(9):1422-1425.
[9]王子文,张航向,任平.哈伯因治疗阿尔茨海默病的临床对比研究[J].医学信息,2000,13(8):462.
[10]韩文霞,宋涛,李伟泽,等.产石杉碱甲新资源研究方法探索[J].海南医学,2015,26(15):2275-2278.
[11] Ward J,Caprio V. A radical mediated approach to thecore structure of huperzine A[J]. Tetrahedron Letters,2006,47:553-556.
[12]韩文霞,宋涛,杨时珍,等.野生蛇足石杉产生物碱和石杉碱甲内生真菌分离鉴定[J].国际药学研究杂志,2015,42(4):507-512.
[13]闵长莉,汪学军.蛇足石杉产石杉碱甲内生真菌的分离鉴定[J].天然产物研究与开发,2013,25(5):590-593.
[14]鞠錾,王峻,潘胜利,等.4种石杉科植物中产生石杉碱甲的内生真菌的分离及初步鉴定和HPLC测定石杉碱甲的含量[J].复旦学报(医学版),2009,36(4):445-449.
[15]赖政,汪娣,汪漄,等.具有乙酰胆碱酯酶抑制活性的蛇足石杉内生真菌分子鉴定[J].菌物学报,2014,33(4):858-866.
[16] Zhang Z B,Zeng Q G,Yan R M,et al.Endophytic fungusCladosporium cladosporioides LF70 from Huperzia serrataproduces huperzine A[J].World J Microbiol Biotechnol,2010,doi:10.1007/s11274-010-0476-6.
[17]邵力平.真菌分类学[M].北京:中国林业出版社,1984.
[18]魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979.
[19] Zhang D L,Yang Y F,Castlebury L A,et al.A methodfor the large scale isolation of high transformation eff-iciency fungal genomic DNA[J]. FEMS MicrobiologyLetters,1996,145:261-265.
[20] White T J,Bruns T,Lee S,et al.Amplification and directsequencing of fungal ribosomal RNA genes for phylogen-etics[M].San Diego:Academic Press,1996:315-322.
[21] Yang Z L.Fungal taxonomy in the genomics era:oppor-tunities and challenges[J]. Mycosystema,2013,32(6):931-946.
[22] B arnett H L,Barry B Hunter. Illustrated genera ofimperfect fungi[M]. Fourth Edition,New York:APSPress,1999.
[23]高小明.华南地区斑痣盘菌目物种多样性与该目双基因系统发育学研究[D].合肥:安徽农业大学,2012.
[24]王士娟.黄山风景区斑痣盘菌科物种多样性与散斑壳属分子系统学研究[D].合肥:安徽农业大学,2007.
[25]任安芝,高玉葆.植物内生真菌———一类应用前景广阔的资源微生物[J].微生物学通报,2001,28(6):90-93.
[26]杨晓军,郭海清,高霞,等.内生真菌YD-01次生代谢产物的研究Ⅰ[J].中国药科大学学报,2006,37(5):479-480.
[27]汪涯,曾庆桂,张志斌,等.蛇足石杉内生真菌分离及其抑制乙酰胆碱酯酶活性研究[J].中国中药杂志,2011,36(6):734-740.