ICARDA小麦种质抗条锈资源筛选和抗病基因分析
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摘要
为给中国小麦抗条锈病育种筛选有效抗源,对203份来自ICARDA的小麦种质,利用杨凌人工病圃和天水、江油自然诱发病圃进行成株期条锈病抗性鉴定,利用条锈菌当前流行小种条中32(CYR32)和条中34(CYR34)在温室进行苗期分小种鉴定,利用 Yr5、 Yr9、 Yr10、 Yr15、 Yr17、 Yr18、 Yr26等抗条锈病基因已开发的分子标记进行基因检测。结果表明,203份材料中107份具有稳定的成株期抗性,31份苗期表现为专化抗性。从这些具有稳定抗病性的材料中,检测到抗条锈病基因 Yr9(51份)、 Yr10(2份)、 Yr17(30份)和 Yr18(56份),未检测到 Yr5、 Yr15、 Yr26;有12份抗条锈表现良好的材料未检测到任何基因,推测其可能携带未开发分子标记的已知基因或新基因。因此,这107份具有稳定抗性的材料可用于中国小麦抗条锈病育种,其中12份可能含有未知基因的材料有待进一步的遗传研究。
Screening effective resistance sources of wheat stripe rust and analyzing its resistance genes are of great value to wheat disease resistance breeding in China. For 203 wheat germplasms from ICARDA, adult resistance was identified by using Yangling artificial disease nursery and Tianshui or Jiangyou natural induced disease nursery; race identification was carried out in greenhouse by using 32(CYR32) and 34(CYR34) races of stripe rust currently prevalent; and gene detection was carried out by using molecular markers developed by Yr5, Yr9, Yr10, Yr15, Yr17, Yr18 and Yr26 genes resistant to stripe rust. The results showed that 107 of 203 materials have stable adult resistance and 31 showed specific resistance at seedling stage. Molecular analysis showed that among 107 materials with stable resistance, 51, 2, 30 and 56 materials were detected to carry stripe rust resistance genes Yr9, Yr10, Yr17,and Yr18, respectively, but no resistant material was detected to carry Yr5, Yr15 and Yr26. No gene was detected from another 12 materials with good resistance to stripe rust, presumably carrying known genes with undeveloped molecular markers or new genes. To conclude,107 wheat germplasms with stable resistance were screened from 203 ICARDA wheat germplasms, and 12 of which may contain unknown genes for the future study.
Screening effective resistance sources of wheat stripe rust and analyzing its resistance genes are of great value to wheat disease resistance breeding in China. For 203 wheat germplasms from ICARDA, adult resistance was identified by using Yangling artificial disease nursery and Tianshui or Jiangyou natural induced disease nursery; race identification was carried out in greenhouse by using 32(CYR32) and 34(CYR34) races of stripe rust currently prevalent; and gene detection was carried out by using molecular markers developed by Yr5, Yr9, Yr10, Yr15, Yr17, Yr18 and Yr26 genes resistant to stripe rust. The results showed that 107 of 203 materials have stable adult resistance and 31 showed specific resistance at seedling stage. Molecular analysis showed that among 107 materials with stable resistance, 51, 2, 30 and 56 materials were detected to carry stripe rust resistance genes Yr9, Yr10, Yr17,and Yr18, respectively, but no resistant material was detected to carry Yr5, Yr15 and Yr26. No gene was detected from another 12 materials with good resistance to stripe rust, presumably carrying known genes with undeveloped molecular markers or new genes. To conclude,107 wheat germplasms with stable resistance were screened from 203 ICARDA wheat germplasms, and 12 of which may contain unknown genes for the future study.
引文
[1]WELLINGS C R.Global status of stripe rust:a review of historical and current threats [J].Euphytica,2011,179(S1):129.
[2]康振生,王晓杰,赵杰,等.小麦条锈菌致病性及其变异研究进展[J].中国农业科学,2015,44(17):3440.KANG Z S,WANG X J,ZHAO J,et al.Advances in research of pathogenicity and virulence variation of the wheat stripe rust fungus Puccinia striiformis f.sp.tritic [J].Scientia Agricultura Sinaica,2015,44(17):3440.
[3]BAI B,DU J Y,LU Q L,et al.Effective resistance to wheat stripe rust in a region with high disease pressure [J].Plant Disease,2014,98(7):893.
[4]HAN D J,WANG Q L,CHEN X M,et al.Emerging Yr26 -virulent races of Puccinia striiformis f.triticiare threatening wheat production in the Sichuan basin,China [J].Plant Disease,2015,99(6):754.
[5]刘博,刘太国,章振羽,等.中国小麦条锈菌条中34号的发现及其致病特性[J].植物病理学报,2017,47(5):681.LIU B,LIU T G,ZHANG Z Y,et al.Discovery and pathogenicity of CYR34,a new race of Puccinia striiformis f.sp.triticiin China [J].Acta Phytopathologica Sinica,2017,47(5):681.
[6]DUAN X,TELLIER A,WAN A,et al.Puccinia striiformis f.sp tritici presents high diversity and recombination in the over-summering zone of Gansu,China [J].Mycologia,2010,102(1):49.
[7]周新力,詹刚明,黄丽丽,等.80份国外春小麦种质资源抗条锈性评价[J].中国农业科学,2015,48(8):1519.ZHOU X L,ZHAN G M,HUANG L L,et al.Evaluation of resistance to stripe rust in eighty abroad spring wheat germplasms [J] .Scientia Agricultura Sinaica,2015,48(8):1519.
[8]蒲宗君,颜泽洪,魏育明,等.小麦材料PI31抗条锈性鉴定及其抗性基因SSR标记[J].植物病理学报,2006,36(4):342.PU Z J,YAN Z H,WEI Y M,et al.Identification and SSR mapping of a stripe rust resistance gene in wheat line PI31[J].Acta Phytopathologica Sinica,2006,36(4):342.
[9]PETERSON R F,CAMPBELL A B,HANNAH A E.A diagrammatic scale for estimating rust intensity on leaves and stems of cereals [J].Canadian Journal of Research,1948,26(5):496.
[10]MCNEAL F H,KONZAK C F,SMITH E P,et al.A uniform system for recording and processing cereal research data[M].USDA,Washington:DCGoogle Scholar,1971:34-121.
[11]李北,徐琪,杨宇衡,等.四川盆地条锈菌冬繁区小麦品种(系)抗条锈性评价及基因分析[J].中国农业科学,2017,50(3):415.LI B,XU Q,YANG Y H,et al.Stripe rust resistance and genes in chongqing wheat cultivars and lines [J].Scientia Agricultura Sinaica,2017,50(3):415.
[12]HILLAMBROZ K L,BROWNGUEDIRA G L,FELLERS J P.Modified rapid DNA extraction protocol for high throughput microsatellite analysis in wheat [J].Cell Biology & Molecular Genetics,2002,42(6):2088.
[13]SMITH P H,HADFIELD J,HART N J,et al.STS markers for the wheat yellow rust resistance gene Yr5 suggest a NBS-LRR-type resistance gene cluster [J].Genome,2007,50(3):260.
[14]CLEMENCE M,JIANPING Z,PENG Z,et al.BED-domain-containing immune receptors confer diverse resistance spectra to yellow rust [J].Nature Plants,2018,4(9):666.
[15]CHENG L,YANG Z J,LI G R,et al.Isolation of a new repetitive DNA sequence from secale africanum enables targeting of secale chromatin in wheat background [J].Euphytica,2008,159(1-2):251.
[16]MAGO R,MIAH H,LAWRENCE G J,et al.High-resolution mapping and mutation analysis separate the rust resistance genes Sr31, Lr26 and Yr9 on the short arm of rye chromosome 1 [J].Theoretical & Applied Genetics,2005,112(1):41.
[17]MICHELE,FRICK,RENE,et al.The stripe rust resistance gene Yr10 encodes an evolutionary-conserved and unique CC-NBS-LRR sequence in wheat [J].Molecular Plant,2014,7(12):1750.
[18]LESLEYR M,DIPAK S,KIMBERLEE K,et al.Linkage maps of wheat stripe rust resistance genes Yr5 and Yr15 for use in marker-assisted selection [J].Crop Science,2009,49(5):1788.
[19]KLYMIUK V,YANIV E,HUANG L,et al.Cloning of the wheat Yr15 resistance gene sheds light on the plant tandem kinase-pseudokinase family [J].Nature Communications,2018(9):3747.
[20]HELGUERA M,KHAN I A,KOLMER J,et al.PCR assays for the Lr37-Yr17 -Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines [J].Crop Science,2003,43(5):1842.
[21]LAGUDAH E S,MCFADDEN H,SINGH R P,et al.Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat [J].Theoretical & Applied Genetics,2006,114(1):24.
[22]LAGUDAH E S,KRATTINGER S G,HERRERAFOESSEL S,et al.Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens [J].Theoretical & Applied Genetics,2009,119(5):892.
[23]XIAOJUAN Z,DEJUN H,QINGDONG Z,et al.Fine mapping of wheat stripe rust resistance gene Yr26 based on collinearity of wheat with Brachypodium distachyon and rice [J].Plos One,2013,8(3):8.
[24]马占鸿.中国小麦条锈病研究与防控[J].植物保护学报,2018,45(1):2.MA Z H.Researches and control of wheat stripe rust in China [J].Journal of Plant Protection,2018,45(1):2.
[25]GESSESE M,BARIANA H,WONG D,et al.Molecular mapping of stripe rust resistance gene Yr81 in a common wheat landrace Aus27430 [J].Plant Disease,2019:PDIS06181055RE.
[26]WU J,LIU S,WANG Q,et al.Rapid identification of an adult plant stripe rust resistance gene in hexaploid wheat by high-throughput SNP array genotyping of pooled extremes [J].Theoretical and Applied Genetics,2018,131(1):43.
[27]ZENG Q,WU J,LIU S,et al.A major QTL co-localized on chromosome 6BL and its epistatic interaction for enhanced wheat stripe rust resistance [J].Theoretical and Applied Genetics,2019,132(5):1409.
[28]COBO N,PFLUGER L,CHEN X,et al.Mapping QTL for resistance to new virulent races of wheat stripe rust from two argentinean wheat cultivars [J].Crop Science,2018,58(6):2470.
[29]NSABIYERA V,BARIANA H S,QURESHI N,et al.Characterisation and mapping of adult plant stripe rust resistance in wheat accession Aus27284 [J].Theoretical and Applied Genetics,2018,131(7):1459.
[30]蔡义忠.ICARDA麦类种质资源研究[J].世界农业,1993(11):17.CAI Y Z.ICARDA wheat germplasm resources [J].World Agriculture,1993(11):17.
[31]韩德俊,康振生.中国小麦品种抗条锈病现状及存在问题与对策 [J].植物保护,2018(5):7.HAN D J,KANG Z S.Current status and future strategy in breeding wheat for resistance to stripe rust in China[J].Plant Protection,2018(5):7.
[32]曾庆东,沈川,袁凤平,等.小麦抗条锈病已知基因对中国当前流行小种的有效性分析[J].植物病理学报,2015,45(6):641.ZENG Q D,SHEN C,YUAN F P,et al.The resistance evaluation of the Yr genes to the main prevalent pathotypes of Puccini a striiformis f.sp.tritici in China [J].Acta Phytopathologica Sinica,2015,45(6):641.
[33]SINGH R P,HUERTA-ESPINO J,BHAVANI S,et al.Race non-specific resistance to rust diseases in CIMMYT spring wheats [J].Euphytica,2011,179(S1):175.
[34]李孟凯. Yr10、 Yr18和 Yr36基因在小麦抗条锈病改良中的应用[D].泰安:山东农业大学,2017:1.LI M K.Application of Yr10, Yr18 and Yr36 genes in wheat improvement for stripe rust resistance [D].Taian:Shandong Agricultural University,2017:1
[2]康振生,王晓杰,赵杰,等.小麦条锈菌致病性及其变异研究进展[J].中国农业科学,2015,44(17):3440.KANG Z S,WANG X J,ZHAO J,et al.Advances in research of pathogenicity and virulence variation of the wheat stripe rust fungus Puccinia striiformis f.sp.tritic [J].Scientia Agricultura Sinaica,2015,44(17):3440.
[3]BAI B,DU J Y,LU Q L,et al.Effective resistance to wheat stripe rust in a region with high disease pressure [J].Plant Disease,2014,98(7):893.
[4]HAN D J,WANG Q L,CHEN X M,et al.Emerging Yr26 -virulent races of Puccinia striiformis f.triticiare threatening wheat production in the Sichuan basin,China [J].Plant Disease,2015,99(6):754.
[5]刘博,刘太国,章振羽,等.中国小麦条锈菌条中34号的发现及其致病特性[J].植物病理学报,2017,47(5):681.LIU B,LIU T G,ZHANG Z Y,et al.Discovery and pathogenicity of CYR34,a new race of Puccinia striiformis f.sp.triticiin China [J].Acta Phytopathologica Sinica,2017,47(5):681.
[6]DUAN X,TELLIER A,WAN A,et al.Puccinia striiformis f.sp tritici presents high diversity and recombination in the over-summering zone of Gansu,China [J].Mycologia,2010,102(1):49.
[7]周新力,詹刚明,黄丽丽,等.80份国外春小麦种质资源抗条锈性评价[J].中国农业科学,2015,48(8):1519.ZHOU X L,ZHAN G M,HUANG L L,et al.Evaluation of resistance to stripe rust in eighty abroad spring wheat germplasms [J] .Scientia Agricultura Sinaica,2015,48(8):1519.
[8]蒲宗君,颜泽洪,魏育明,等.小麦材料PI31抗条锈性鉴定及其抗性基因SSR标记[J].植物病理学报,2006,36(4):342.PU Z J,YAN Z H,WEI Y M,et al.Identification and SSR mapping of a stripe rust resistance gene in wheat line PI31[J].Acta Phytopathologica Sinica,2006,36(4):342.
[9]PETERSON R F,CAMPBELL A B,HANNAH A E.A diagrammatic scale for estimating rust intensity on leaves and stems of cereals [J].Canadian Journal of Research,1948,26(5):496.
[10]MCNEAL F H,KONZAK C F,SMITH E P,et al.A uniform system for recording and processing cereal research data[M].USDA,Washington:DCGoogle Scholar,1971:34-121.
[11]李北,徐琪,杨宇衡,等.四川盆地条锈菌冬繁区小麦品种(系)抗条锈性评价及基因分析[J].中国农业科学,2017,50(3):415.LI B,XU Q,YANG Y H,et al.Stripe rust resistance and genes in chongqing wheat cultivars and lines [J].Scientia Agricultura Sinaica,2017,50(3):415.
[12]HILLAMBROZ K L,BROWNGUEDIRA G L,FELLERS J P.Modified rapid DNA extraction protocol for high throughput microsatellite analysis in wheat [J].Cell Biology & Molecular Genetics,2002,42(6):2088.
[13]SMITH P H,HADFIELD J,HART N J,et al.STS markers for the wheat yellow rust resistance gene Yr5 suggest a NBS-LRR-type resistance gene cluster [J].Genome,2007,50(3):260.
[14]CLEMENCE M,JIANPING Z,PENG Z,et al.BED-domain-containing immune receptors confer diverse resistance spectra to yellow rust [J].Nature Plants,2018,4(9):666.
[15]CHENG L,YANG Z J,LI G R,et al.Isolation of a new repetitive DNA sequence from secale africanum enables targeting of secale chromatin in wheat background [J].Euphytica,2008,159(1-2):251.
[16]MAGO R,MIAH H,LAWRENCE G J,et al.High-resolution mapping and mutation analysis separate the rust resistance genes Sr31, Lr26 and Yr9 on the short arm of rye chromosome 1 [J].Theoretical & Applied Genetics,2005,112(1):41.
[17]MICHELE,FRICK,RENE,et al.The stripe rust resistance gene Yr10 encodes an evolutionary-conserved and unique CC-NBS-LRR sequence in wheat [J].Molecular Plant,2014,7(12):1750.
[18]LESLEYR M,DIPAK S,KIMBERLEE K,et al.Linkage maps of wheat stripe rust resistance genes Yr5 and Yr15 for use in marker-assisted selection [J].Crop Science,2009,49(5):1788.
[19]KLYMIUK V,YANIV E,HUANG L,et al.Cloning of the wheat Yr15 resistance gene sheds light on the plant tandem kinase-pseudokinase family [J].Nature Communications,2018(9):3747.
[20]HELGUERA M,KHAN I A,KOLMER J,et al.PCR assays for the Lr37-Yr17 -Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines [J].Crop Science,2003,43(5):1842.
[21]LAGUDAH E S,MCFADDEN H,SINGH R P,et al.Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat [J].Theoretical & Applied Genetics,2006,114(1):24.
[22]LAGUDAH E S,KRATTINGER S G,HERRERAFOESSEL S,et al.Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens [J].Theoretical & Applied Genetics,2009,119(5):892.
[23]XIAOJUAN Z,DEJUN H,QINGDONG Z,et al.Fine mapping of wheat stripe rust resistance gene Yr26 based on collinearity of wheat with Brachypodium distachyon and rice [J].Plos One,2013,8(3):8.
[24]马占鸿.中国小麦条锈病研究与防控[J].植物保护学报,2018,45(1):2.MA Z H.Researches and control of wheat stripe rust in China [J].Journal of Plant Protection,2018,45(1):2.
[25]GESSESE M,BARIANA H,WONG D,et al.Molecular mapping of stripe rust resistance gene Yr81 in a common wheat landrace Aus27430 [J].Plant Disease,2019:PDIS06181055RE.
[26]WU J,LIU S,WANG Q,et al.Rapid identification of an adult plant stripe rust resistance gene in hexaploid wheat by high-throughput SNP array genotyping of pooled extremes [J].Theoretical and Applied Genetics,2018,131(1):43.
[27]ZENG Q,WU J,LIU S,et al.A major QTL co-localized on chromosome 6BL and its epistatic interaction for enhanced wheat stripe rust resistance [J].Theoretical and Applied Genetics,2019,132(5):1409.
[28]COBO N,PFLUGER L,CHEN X,et al.Mapping QTL for resistance to new virulent races of wheat stripe rust from two argentinean wheat cultivars [J].Crop Science,2018,58(6):2470.
[29]NSABIYERA V,BARIANA H S,QURESHI N,et al.Characterisation and mapping of adult plant stripe rust resistance in wheat accession Aus27284 [J].Theoretical and Applied Genetics,2018,131(7):1459.
[30]蔡义忠.ICARDA麦类种质资源研究[J].世界农业,1993(11):17.CAI Y Z.ICARDA wheat germplasm resources [J].World Agriculture,1993(11):17.
[31]韩德俊,康振生.中国小麦品种抗条锈病现状及存在问题与对策 [J].植物保护,2018(5):7.HAN D J,KANG Z S.Current status and future strategy in breeding wheat for resistance to stripe rust in China[J].Plant Protection,2018(5):7.
[32]曾庆东,沈川,袁凤平,等.小麦抗条锈病已知基因对中国当前流行小种的有效性分析[J].植物病理学报,2015,45(6):641.ZENG Q D,SHEN C,YUAN F P,et al.The resistance evaluation of the Yr genes to the main prevalent pathotypes of Puccini a striiformis f.sp.tritici in China [J].Acta Phytopathologica Sinica,2015,45(6):641.
[33]SINGH R P,HUERTA-ESPINO J,BHAVANI S,et al.Race non-specific resistance to rust diseases in CIMMYT spring wheats [J].Euphytica,2011,179(S1):175.
[34]李孟凯. Yr10、 Yr18和 Yr36基因在小麦抗条锈病改良中的应用[D].泰安:山东农业大学,2017:1.LI M K.Application of Yr10, Yr18 and Yr36 genes in wheat improvement for stripe rust resistance [D].Taian:Shandong Agricultural University,2017:1