3个辣木品种耐寒性评价
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摘要
[目的]对3个辣木品种的耐寒性进行评价。[方法]采用紫外分光光度法测定不同温度(25、5、0、-2℃)处理下传统辣木、改良辣木PKM1和改良辣木PKM2叶片的丙二醛(malondialdehyde, MDA)、可溶性糖、游离脯氨酸(proline, Pro)含量和超氧化物歧化酶(superoxide dismutase, SOD)活性,并应用模糊隶属函数评价法对其耐寒性进行综合评价。[结果]随着温度的降低,3个辣木品种MDA、Pro含量和SOD活性总体呈先下降后升高的趋势,处理温度为-2℃时,传统辣木与改良辣木PKM1、PKM2差异显著,改良辣木PKM1与PKM2间差异不显著;可溶性糖含量总体呈先升高后下降的趋势,不同温度处理下各品种间差异均不显著。[结论]依据各生理指标的平均隶属度,3个辣木品种耐寒性依次为:改良辣木PKM2>改良辣木PKM1>传统辣木。
[Purpose] The cold resistance of three Moringa oleifera cultivars was evaluated in order. [Method] Changes in the contents of malondialdehyde(MDA), soluble sugar, free proline(Pro), and superoxide dismutase(SOD) activity in the leaves of three M.oleifera cultivars under different temperatures(25, 5, 0, -2 ℃)were determined by UV spectrophotometry, and a comprehensive evaluation of cold resistance was conducted by the fuzzy membership function evaluation method. [Result] Under decreasing temperatures, the contents of MDA and Pro and SOD activity in the three M.oleifera cultivars decreased first and then increased. At -2 ℃, these contents differed significantly between the traditional cultivar Moringa and the modified cultivars Moringa PKM1 or PKM2. There was no significant difference between PKM1 and PKM2. The soluble sugar content increased first and then decreased. There was no significant difference among the three cultivars under different temperature treatments. [Conclusion] Based on the average membership of each physiological index, the cold resistance of the three M.oleifera cultivars was in the order of modified Moringa PKM2>modified Moringa PKM1>traditional Moringa.
[Purpose] The cold resistance of three Moringa oleifera cultivars was evaluated in order. [Method] Changes in the contents of malondialdehyde(MDA), soluble sugar, free proline(Pro), and superoxide dismutase(SOD) activity in the leaves of three M.oleifera cultivars under different temperatures(25, 5, 0, -2 ℃)were determined by UV spectrophotometry, and a comprehensive evaluation of cold resistance was conducted by the fuzzy membership function evaluation method. [Result] Under decreasing temperatures, the contents of MDA and Pro and SOD activity in the three M.oleifera cultivars decreased first and then increased. At -2 ℃, these contents differed significantly between the traditional cultivar Moringa and the modified cultivars Moringa PKM1 or PKM2. There was no significant difference between PKM1 and PKM2. The soluble sugar content increased first and then decreased. There was no significant difference among the three cultivars under different temperature treatments. [Conclusion] Based on the average membership of each physiological index, the cold resistance of the three M.oleifera cultivars was in the order of modified Moringa PKM2>modified Moringa PKM1>traditional Moringa.
引文
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[14] 杨春祥,李宪利,高东升,等.低温胁迫对油桃花器官膜脂过氧化和保护酶活性的影响[J].果树学报,2005,22(1):69-71.
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[17] 姜慧芳,任小平.干旱胁迫对花生叶片SOD活性和蛋白质的影响[J].作物学报,2004,30(2):169-174.
[18] 冯昌军,罗新义,沙伟,等.低温胁迫对苜蓿品种幼苗SOD、POD活性和脯氨酸含量的影响[J].草业科学,2005,22(6):29-32.
[2] 许敏,赵三军,宋晖,等.辣木的研究进展[J].食品科学,2016,37(23):291-301.
[3] DOORA P D,SINGH B P,TANDON S.Vitamin C content in Moringa pod vegetable[J].Current Science,1975,44:31.
[4] DAHOT M U.Vitamin contents of the flowers and seeds of Moringa oleifera[J].Pakistan Journal of Biochemistry,1988,21(1/2):21-24.
[5] 马春花,邵建辉,田洋,等.云南辣木种植区气候条件适宜性初步评价[J].热带农业科学,2016,36(6):28-32.
[6] 王鹏,陈婷,孙加节,等.辣木在动物饲料中应用的研究进展[J].动物营养学报,2018,30(7):2 488-2 495.
[7] ASGHARI G,PALIZBAN A B,BAKHSHAEI B.Quantitative analysis of the nutritional components in leaves and seeds of the Persian moringa (Forssk.) Fiori[J].Pharmacognosy Research,2015,7(3):242-248.
[8] 高婷婷.10个白三叶品种耐寒性评价研究[D].晋中:山西农业大学,2014.
[9] 崔帅.泰安地区7个常绿阔叶树种(品种)耐寒性研究[D].泰安:山东农业大学,2013.
[10] 韩瑞超,邱乾栋,臧德奎,等.低温胁迫下桂花品种的生理变化及耐寒性评价[J].山东农业科学,2011(12):27-30.
[11] 卢会文,杨清辉,肖关丽,等.云南省主栽甘蔗品种抗旱性鉴定[J].亚热带农业研究,2012,8(1):8-12.
[12] 李彩霞,郑冬梅,林碧英,等.盐胁迫下外源激素对苦瓜幼苗抗逆性的影响[J].亚热带农业研究,2017,13(3):171-176.
[13] 李凯,杨建平,马宁,等.低温处理对裸仁南瓜幼苗抗寒性指标的影响[J].山东农业科学,2009(1):52-54.
[14] 杨春祥,李宪利,高东升,等.低温胁迫对油桃花器官膜脂过氧化和保护酶活性的影响[J].果树学报,2005,22(1):69-71.
[15] 吴继林.大湖竹种园丛生竹种的收集及其耐寒性评价研究[J].竹子研究汇刊,2008,27(1):19-26.
[16] 马娟,王飞,梁宗锁.八种常绿阔叶树的抗寒性研究[J].西北林学院学报,2008,23(5):26-29.
[17] 姜慧芳,任小平.干旱胁迫对花生叶片SOD活性和蛋白质的影响[J].作物学报,2004,30(2):169-174.
[18] 冯昌军,罗新义,沙伟,等.低温胁迫对苜蓿品种幼苗SOD、POD活性和脯氨酸含量的影响[J].草业科学,2005,22(6):29-32.