2株致犊牛腹泻大肠杆菌的分离鉴定和药敏试验
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摘要
赣西某肉牛养殖场有犊牛出现消瘦、食欲下降和排腥臭稀便等症状,为确定病原采集了腹泻犊牛的新鲜粪便,采用Bio-X犊牛消化道五联原位快速诊断试剂盒进行快速诊断,同时进行病原菌的分离与鉴定;为了确定病原菌对抗菌药的敏感性,选取了磺胺嘧啶钠、青霉素、链霉素、土霉素和氧氟沙星等多种抗菌药对病原菌进行体外抑菌试验。通过临床快速诊断与实验室诊断确定该病是由大肠杆菌引起的,体外抑菌试验结果显示2株分离菌对环丙沙星高度敏感,对青霉素和头孢拉定等抗菌药表现完全耐药。该养殖场依据药敏试验结果合理用药并采取多种防治措施,数日后无新发病例并且发病牛的病情得到较好控制。
Calves in a beef cattle farm in western Jiangxi Province were reported to have clinical symptoms of emaciation, loss of appetite and diarrhea. In order to identify the pathogenic agent associated with this disease, fresh fecal samples of calves with diarrhea were recovered, Bio-x five-line in situ rapid diagnosis kit of calf digestive tract was used for rapid diagnosis, isolation and identification of pathogenic bacteria were carried out, and in vitro susceptibility of the isolates against the commonly used antibiotics in veterinary clinic was tested. Rapid clinical diagnosis and laboratory diagnosis confirmed that the disease was caused by infection of Escherichia coli. The two isolates were highly sensitive to ciprofloxacin and completely resistant to penicillin,cefradine and other antibiotics. The diarrhea of calves in this cattle farm were well controlled using the antibiotic recommended by this study.
Calves in a beef cattle farm in western Jiangxi Province were reported to have clinical symptoms of emaciation, loss of appetite and diarrhea. In order to identify the pathogenic agent associated with this disease, fresh fecal samples of calves with diarrhea were recovered, Bio-x five-line in situ rapid diagnosis kit of calf digestive tract was used for rapid diagnosis, isolation and identification of pathogenic bacteria were carried out, and in vitro susceptibility of the isolates against the commonly used antibiotics in veterinary clinic was tested. Rapid clinical diagnosis and laboratory diagnosis confirmed that the disease was caused by infection of Escherichia coli. The two isolates were highly sensitive to ciprofloxacin and completely resistant to penicillin,cefradine and other antibiotics. The diarrhea of calves in this cattle farm were well controlled using the antibiotic recommended by this study.
引文
[1]王丹阳,王旭荣,张康,等.牛病毒性腹泻病毒、大肠杆菌和奇异变形杆菌混合感染致犊牛腹泻的研究[J].中国畜牧兽医,2018,45(1):189-195.
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[11]齐宇,王开,伊淑帅,等.产肠毒素大肠杆菌K88诱发BALB/c鼠肠炎模型的建立及评价[J].中国预防兽医学报,2016,38(1):19-22.
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[14]李海花,朱琪,王世琼,等.产肠毒素大肠杆菌K88诱导仔猪炎症反应的分子机制[J].中国畜牧兽医,2107,44(1):262-267.
[15]陈杖榴,袁宗辉,曾振灵,等.兽医药理学[M].北京:中国农业出版社,2009:198-199.
[16]杜淑环,刘秀明,万秋,等.天蚕素抗菌肽的研究进展[J].动物营养学报,2012,24(1):41-47.
[17]姜琛璐,汤承,骞宇,等.黄芪多糖免疫调节作用研究进展[J].食品科学,2013,34(11):327-332.
[18]刘洪明.益生菌—黄芪多糖合生元的研制及其对腹泻犊牛肠道菌群结构影响的研究[D].杨凌:西北农林科技大学,2009.
[19]张陆,蒋月,盛鹏飞,等.腹泻犊牛大肠杆菌血清型抗菌药物耐药性耐药基因鉴定及脉冲场凝胶电泳分型[J].中国兽医杂志,2013,49(9):15-17.
[2]钟细平.新生牛犊腹泻的防治[J].中国畜牧兽医文摘,2016,32(4):112.
[3]曹明泽,朱阵,王磊,等.犊牛腹泻概述[J].黑龙江畜牧兽医,2016(14):116-119.
[4]荔霞,王胜义,刘永明,等.犊牛腹泻病因及其药物防治研究进展[J].中国畜牧兽医,2010,37(10):161-165.
[5]方光远,张莉,陆宇超,等.犊牛腹泻大肠杆菌的分离鉴定和耐药性检测[J].中国畜牧兽医,2014,41(9):273-277.
[6]BUCHANAN R E, GIBBONS N E.伯杰细菌鉴定手册[M].8版.北京:科学出版社,1984:667-675.
[7]NCCLC. Performance standards for antimicrobial disk and dilution susceptibilty tests for bacteria isolated from animals:approved standard[S].Wayne:Clinical and Laboratory Standards Institute,2009.
[8]郭沈涛,于辉,林旭埜,等.犊牛病原性腹泻及疫苗研究进展[J].中国畜牧兽医,2017,44(12):3618-3624.
[9]蒋磊,周明旭,夏芃芃,等.产肠毒素大肠杆菌感染的分子致病机制[J].中国兽医学报,2014,34(9):1551-1560.
[10]UMPIRREZ A,ACQUISTAPACE S,FERNNDEZ S,et al. Prevalence of Escherichia coli adhesion-related genes in neonatal calf diarrhea in Uruguay[J].J Infect Dev Ctrie,2016,10(5):472-477.
[11]齐宇,王开,伊淑帅,等.产肠毒素大肠杆菌K88诱发BALB/c鼠肠炎模型的建立及评价[J].中国预防兽医学报,2016,38(1):19-22.
[12]JORES J,RUMER L,KIESSLING S,et al. A novel locus of enterocyte effacement(LEE)pathogenicity island inserted at phe V in bovine Shiga toxin-producing Escherichia coli strain O103:H 2[J].FEMS Micro-biol Lett,2001,204(1):75-79.
[13]BACH S, de ALMEIDA A,CARNIEL E.The Yersinia high-pathogenicity island is present in different members of the family Enterobacteriaceae[J].FEMS Microbiol Lett,2000,183(2):289-294.
[14]李海花,朱琪,王世琼,等.产肠毒素大肠杆菌K88诱导仔猪炎症反应的分子机制[J].中国畜牧兽医,2107,44(1):262-267.
[15]陈杖榴,袁宗辉,曾振灵,等.兽医药理学[M].北京:中国农业出版社,2009:198-199.
[16]杜淑环,刘秀明,万秋,等.天蚕素抗菌肽的研究进展[J].动物营养学报,2012,24(1):41-47.
[17]姜琛璐,汤承,骞宇,等.黄芪多糖免疫调节作用研究进展[J].食品科学,2013,34(11):327-332.
[18]刘洪明.益生菌—黄芪多糖合生元的研制及其对腹泻犊牛肠道菌群结构影响的研究[D].杨凌:西北农林科技大学,2009.
[19]张陆,蒋月,盛鹏飞,等.腹泻犊牛大肠杆菌血清型抗菌药物耐药性耐药基因鉴定及脉冲场凝胶电泳分型[J].中国兽医杂志,2013,49(9):15-17.
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