江孜沙棘叶片SPAD值与叶绿素含量相关性研究
|
摘要
为了研究江孜沙棘(Hippophae gyantsensis)SPAD值与叶绿素的关系,应用分光光度计法与SPAD叶绿仪法分别测定了江孜沙棘叶片的叶绿素含量和SPAD值,建立SPAD值与叶绿素a、叶绿素b及总叶绿素的指数模型、线性模型、对数模型、多项式模型和乘幂模型的拟合方程,来确定最佳拟合模型。结果表明:江孜沙棘叶片SPAD值与总叶绿素、叶绿素a及叶绿素b之间存在极显著正相关;SPAD值与叶绿素a、叶绿素b及总叶绿素的最佳拟合方程分别是多项式模型(y=0.0014x~2-0.093x+2.3438)、线性模型(y=0.0083x-0.1203)和指数模型(y=0.2484e0.0358x)。通过江孜沙棘叶片各叶绿素实测值与预测值的统计检验表明,叶绿素a和总叶绿素的实测值与预测值之间差异不显著(P>0.05),故可通过模型来预测江孜沙棘叶片叶绿素的含量。
In order to investigate the relationship between SPAD value and chlorophyll of Hippophae gyantsensis, the chlorophyll content and SPAD Chlorophyll meter of Hippophae gyantsensis leaves were determined by spectrophotometer and SPAD values, then the SPAD value and chlorophyll a, chlorophyll b and total chlorophyll index models, the linear model, the logarithmic model, the polynomial model, and the fitting equation of the power model were established to determine the best fit model. The results showed that there was a significant positive correlation between SPAD value and chlorophyll a, chlorophyll b and total chlorophyll in the leaves of Hippophae gyantsensis. The best fitting equations of SPAD value and chlorophyll a, chlorophyll b and total chlorophyll were polynomial model(y=0.0014x~2-0.093x+2.3438), linear model(y=0.0083x-0.1203), and index models(y=0.2484e0.0358x). The statistical test for measured and predicted leaf chlorophyll of Hippophae gyantsensis showed that the difference between the measured value and the predicted value of chlorophyll a and total chlorophyll was not significant. Thus, the chlorophyll content of Hippophae gyantsensis can be predicted by model.
In order to investigate the relationship between SPAD value and chlorophyll of Hippophae gyantsensis, the chlorophyll content and SPAD Chlorophyll meter of Hippophae gyantsensis leaves were determined by spectrophotometer and SPAD values, then the SPAD value and chlorophyll a, chlorophyll b and total chlorophyll index models, the linear model, the logarithmic model, the polynomial model, and the fitting equation of the power model were established to determine the best fit model. The results showed that there was a significant positive correlation between SPAD value and chlorophyll a, chlorophyll b and total chlorophyll in the leaves of Hippophae gyantsensis. The best fitting equations of SPAD value and chlorophyll a, chlorophyll b and total chlorophyll were polynomial model(y=0.0014x~2-0.093x+2.3438), linear model(y=0.0083x-0.1203), and index models(y=0.2484e0.0358x). The statistical test for measured and predicted leaf chlorophyll of Hippophae gyantsensis showed that the difference between the measured value and the predicted value of chlorophyll a and total chlorophyll was not significant. Thus, the chlorophyll content of Hippophae gyantsensis can be predicted by model.
引文
[1]徐芬芬,叶利民,徐卫红等.小白菜叶绿素含量的测定方法比较[J].北方园艺,2010(23):32-34.
[2]曾建敏,姚恒,李天福等.烤烟叶片叶绿素含量的测定及其与SPAD值的关系[J].分子植物育种,2009,7(01):56-62.
[3]王瑞,陈永忠,陈隆升等.油茶叶片SPAD值与叶绿素含量的相关分析[J].中南林业科技大学学报,2013,33(02):77-80.
[4]王薇,宋廷宇,王艳等.番茄叶片SPAD值与叶绿素含量的相关性分析[J].北方园艺,2013(23):12-15.
[5]苏云松,郭华春,陈伊里.马铃薯叶片SPAD值与叶绿素含量及产量的相关性研究[J].西南农业学报,2007,20(04):690-693.
[6]张文英,王凯华.甘蓝型油菜SPAD值与叶绿素含量关系分析[J].中国农学通报,2012,28(21):92-95.
[7]李辉,白丹,张卓等.羊草叶片SPAD值与叶绿素含量的相关分析[J].中国农学通报,2012,28(02):27-30.
[8]李敏夏,张林森,李丙智等.苹果叶片高光谱特性与叶绿素含量和SPAD值的关系[J].西北林学院学报,2010,25(02):35-39.
[9]姜丽芬,石福臣,王化田等.叶绿素计SPAD-502在林业上应用[J].生态学杂志,2005,24(12):1543-1548.
[10]李田,韩霞,魏永阳等.三种板栗叶片SPAD值与叶绿素含量相关性分析[J].山东林业科技,2017,47(01):23-27.
[11]宋廷宇,吴春燕,常雪等.西葫芦叶片SPAD值与叶绿素含量相关性分析[J].吉林农业科学,2014,39(03):67-70.
[12]Deborah K.Reed,Yaacov Petscher,Adrea J.The Contrib ution of General Reading Ability to Science Achieve ment[J].Reading Research Quarterly,2017,52(2):253-266.
[13]F.Allen Dray,Ted D.Center,Elizabeth D.Mattison.In situ estimates of waterhyacinth leaf tissue nitrogen usi ng a SPAD-502 chlorophyll meter[J].Aquatic Botany,2012,100:72-75.
[14]邱念伟,王修顺,杨发斌等.叶绿素的快速提取与精密测定[J].植物学报,2016,51(05):667-678.
[15]唐银凤.叶绿素含量测定中的误差分析[J].南京理工大学学报,1997,21(02):38-40.
[16]严衍禄,刘心生.叶绿素测定方法的研究[J].北京农业大学学报,1982,8(02):53-67.
[17]陈宝燕.棉花氮素无损诊断中SPAD值与土壤和植株含氮量的关系[D].乌鲁木齐:.新疆农业大学,2011.
[18]艾天成,李方敏,周治安等.作物叶片叶绿素含量与SPAD值相关性研究[J].湖北农学院学报,2000,20(01):6-8.
[19]刘井良,王丽华,李杰勤等.10个黑麦草品种叶片SPAD值,叶绿素含量和蛋白质含量的相关性研究[J].中国农学通报,2012,28(27):83-86.
[20]苏云松,郭华春,杨雪兰.甘薯,薯蓣和魔芋叶片SPAD值与叶绿素含量的相关性研究[J].西南农业学报,2009,22(01):64-66.
[21]陈防,鲁剑巍.SPAD-502叶绿素计在作物营养快速诊断上的应用初探[J].湖北农业科学,1996(02):31-34.
[22]刘西军,陈静,徐小牛等.桂花叶片SPAD、叶绿素含量和比叶重特征[J].安徽农业大学学报,2013,40(01):51-54.
[23]宋廷宇,何自涵,程艳等.菜心叶片SPAD值与叶绿素含量的相关性分析[J].东北农业科学,2017,42(01):34-37.
[24]宋廷宇,陈赫楠,常雪等.2个薄皮甜瓜叶片SPAD值与叶绿素含量的相关性分析[J].江苏农业科学,2014,42(04):127-129.
[25]陈红刚,赵惠,张晓玲等.菘蓝叶片叶绿素含量与SPAD值的相关性研究[J].中国中医药科技,2017,24(01):44-45+55.
[26]Sibley J L,Eakes D J,Gilliam C H.Foliar SPAD-502metervalues,nitrogen levels,and extractable chlorophyll for red maple selections[J].Hort Science,1996,31(6):468-470.
[27]艾天成,李方敏,周治安等.作物叶片叶绿素含量与SPAD值相关性研究[J].湖北农学院学报,2000,20(01):6-8.
[28]刘孝良,赫英姿,于庭洪等.柞树叶片SPAD值与叶绿素含量相关性的分析[J].北方蚕业,2016,37(04):16-19+24.
[29]陈景蕊,潘静.不同品种葡萄叶片SPAD值与叶绿素含量相关性分析[J].北方园艺,2015(19):42-46.
[2]曾建敏,姚恒,李天福等.烤烟叶片叶绿素含量的测定及其与SPAD值的关系[J].分子植物育种,2009,7(01):56-62.
[3]王瑞,陈永忠,陈隆升等.油茶叶片SPAD值与叶绿素含量的相关分析[J].中南林业科技大学学报,2013,33(02):77-80.
[4]王薇,宋廷宇,王艳等.番茄叶片SPAD值与叶绿素含量的相关性分析[J].北方园艺,2013(23):12-15.
[5]苏云松,郭华春,陈伊里.马铃薯叶片SPAD值与叶绿素含量及产量的相关性研究[J].西南农业学报,2007,20(04):690-693.
[6]张文英,王凯华.甘蓝型油菜SPAD值与叶绿素含量关系分析[J].中国农学通报,2012,28(21):92-95.
[7]李辉,白丹,张卓等.羊草叶片SPAD值与叶绿素含量的相关分析[J].中国农学通报,2012,28(02):27-30.
[8]李敏夏,张林森,李丙智等.苹果叶片高光谱特性与叶绿素含量和SPAD值的关系[J].西北林学院学报,2010,25(02):35-39.
[9]姜丽芬,石福臣,王化田等.叶绿素计SPAD-502在林业上应用[J].生态学杂志,2005,24(12):1543-1548.
[10]李田,韩霞,魏永阳等.三种板栗叶片SPAD值与叶绿素含量相关性分析[J].山东林业科技,2017,47(01):23-27.
[11]宋廷宇,吴春燕,常雪等.西葫芦叶片SPAD值与叶绿素含量相关性分析[J].吉林农业科学,2014,39(03):67-70.
[12]Deborah K.Reed,Yaacov Petscher,Adrea J.The Contrib ution of General Reading Ability to Science Achieve ment[J].Reading Research Quarterly,2017,52(2):253-266.
[13]F.Allen Dray,Ted D.Center,Elizabeth D.Mattison.In situ estimates of waterhyacinth leaf tissue nitrogen usi ng a SPAD-502 chlorophyll meter[J].Aquatic Botany,2012,100:72-75.
[14]邱念伟,王修顺,杨发斌等.叶绿素的快速提取与精密测定[J].植物学报,2016,51(05):667-678.
[15]唐银凤.叶绿素含量测定中的误差分析[J].南京理工大学学报,1997,21(02):38-40.
[16]严衍禄,刘心生.叶绿素测定方法的研究[J].北京农业大学学报,1982,8(02):53-67.
[17]陈宝燕.棉花氮素无损诊断中SPAD值与土壤和植株含氮量的关系[D].乌鲁木齐:.新疆农业大学,2011.
[18]艾天成,李方敏,周治安等.作物叶片叶绿素含量与SPAD值相关性研究[J].湖北农学院学报,2000,20(01):6-8.
[19]刘井良,王丽华,李杰勤等.10个黑麦草品种叶片SPAD值,叶绿素含量和蛋白质含量的相关性研究[J].中国农学通报,2012,28(27):83-86.
[20]苏云松,郭华春,杨雪兰.甘薯,薯蓣和魔芋叶片SPAD值与叶绿素含量的相关性研究[J].西南农业学报,2009,22(01):64-66.
[21]陈防,鲁剑巍.SPAD-502叶绿素计在作物营养快速诊断上的应用初探[J].湖北农业科学,1996(02):31-34.
[22]刘西军,陈静,徐小牛等.桂花叶片SPAD、叶绿素含量和比叶重特征[J].安徽农业大学学报,2013,40(01):51-54.
[23]宋廷宇,何自涵,程艳等.菜心叶片SPAD值与叶绿素含量的相关性分析[J].东北农业科学,2017,42(01):34-37.
[24]宋廷宇,陈赫楠,常雪等.2个薄皮甜瓜叶片SPAD值与叶绿素含量的相关性分析[J].江苏农业科学,2014,42(04):127-129.
[25]陈红刚,赵惠,张晓玲等.菘蓝叶片叶绿素含量与SPAD值的相关性研究[J].中国中医药科技,2017,24(01):44-45+55.
[26]Sibley J L,Eakes D J,Gilliam C H.Foliar SPAD-502metervalues,nitrogen levels,and extractable chlorophyll for red maple selections[J].Hort Science,1996,31(6):468-470.
[27]艾天成,李方敏,周治安等.作物叶片叶绿素含量与SPAD值相关性研究[J].湖北农学院学报,2000,20(01):6-8.
[28]刘孝良,赫英姿,于庭洪等.柞树叶片SPAD值与叶绿素含量相关性的分析[J].北方蚕业,2016,37(04):16-19+24.
[29]陈景蕊,潘静.不同品种葡萄叶片SPAD值与叶绿素含量相关性分析[J].北方园艺,2015(19):42-46.