温度驯化对树麻雀糖原含量及脱支酶活性影响
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摘要
为了研究温度驯化对树麻雀糖原含量及脱支酶活性的影响,将野外捕获的树麻雀按体质量随机分为高温组(35℃)、低温组(5℃)和对照组(25℃),驯化4周,探讨不同温度对树麻雀体质量、器官质量、基础代谢率、体脂质量分数(索氏抽提法)、糖原含量(硫酸蒽酮法)和脱支酶活性等生理生化指标的影响.结果表明,(1)低温组随着驯化的进行,体质量逐渐下降,至驯化结束共下降0.80g,与对照组比较差异极显著(p<0.01);而高温组的体质量随着驯化的进行逐渐上升,驯化结束后共增长了0.60g,与对照组比较差异极显著(p<0.01).(2)高温组的基础代谢率逐渐降低,在驯化0～1周内基础代谢率急剧下降,第4周驯化结束时与初始相比减小了0.39 mL O2/(g·h),和对照组相较差异极显著(p<0.01);低温组的基础代谢率逐渐增高,在驯化1～3周内基础代谢率大幅增加,第4周驯化结束时与初始相比增加了0.51 mL O2/(g·h),与对照组比较差异极显著(p<0.01).(3)低温组体脂质量分数比对照组低3.17%,高温组体脂质量分数比对照组高1.46%,3组间的差异极显著(p<0.01).(4)3组间肌肉内脱支酶活性均无显著性差异(p>0.05);而肝脏内脱支酶活性低温组与高温组差异极显著(p<0.01).(5)低温组、高温组与对照组比较肌糖原含量差异均极显著(p<0.01);3组间肝糖原含量差异均极显著(p<0.01).(6)高温组和低温组肾、胰、脾的干质量与对照组无显著性差异(p>0.05).揭示温度是影响树麻雀产热特征性变化的一个主要环境因素,高温环境下,树麻雀通过降低代谢率和对食物的消化吸收速率,降低体内脱支酶活性来减少能量耗费,以适应环境温度的变化.
In order to study the effects of temperature acclimation on the glycogen content and debranching enzyme activity of Passer montanus.Select four weeks domestication Passer montanus which caught in the wild to test the effects of temperature on their body weight,organ mass,basal metabolic rate,body fat mass fraction(by soxhlet extractionmemthod),glycogen content(Sulfuric acid anthrone method),debranching enzyme activity and other physiological and biochemical indicators.Three treatments were prepared,they were high-temperature group(35 ℃),low-temperature group(5 ℃)and the control group(25 ℃).The results showed that,(1)After domestication,Passer montanus body weight in low-temperature group decreased 0.80 g in total,body mass reached very significant level compared to the control group(p<0.01);body weight in high-temperature group increased 0.60 g in total,very significant level compared to the control group(p<0.01).(2)Basal metabolic rate in high-temperature group in 0～1 weeks got obvious decrease,after four weeks domestication decrease amount of 0.39 mL O2/(g·h),it reached very significant level compared to the control group(p<0.01);basal metabolic rate in low-temperature group in 1～3 weeks got obvious increase,after four weeks domestication increase amount of 0.51 mL O2/(g·h),it reached very significant level compared to the control group(p<0.01).(3)Body fat mass fraction in low-temperature group was lower 3.17% than that in control group,body fat mass fraction in high-temperature group was higher 1.46% than that in control group,the body fat mass fractions of tree sparrows among the low temperature group,the three groups were significantly different(p<0.01).(4)The body debranching enzyme activity in muscle of tree sparrows among the low temperature group,the three groups were no significantly different(p>0.05);low-temperature group debranching enzyme activity in liver was very significant compared to the high-temperature group(p<0.01).(5)Control group muscle glycogen was very significant compared to the low-temperature group and high-temperature group(p<0.01);Three groups liver glycogen was very significant compared to each other(p<0.01).(6)The dry weight of kidney,pancreas and spleen in low-temperature group and high-temperature group were not reached very significant level compared them with the control group(p>0.05).Temperature was the main environmental factors affecting the change of thermogenesis at the condition of the outside temperature.In the high-temperature,Passer montanus could adapt itself to the change of temperature by lowering body weight,basal metabolic rate,debranching enzyme activity in liver and muscle tissues to compensate the energy consumption from a series of physiological and biochemical reactions.
In order to study the effects of temperature acclimation on the glycogen content and debranching enzyme activity of Passer montanus.Select four weeks domestication Passer montanus which caught in the wild to test the effects of temperature on their body weight,organ mass,basal metabolic rate,body fat mass fraction(by soxhlet extractionmemthod),glycogen content(Sulfuric acid anthrone method),debranching enzyme activity and other physiological and biochemical indicators.Three treatments were prepared,they were high-temperature group(35 ℃),low-temperature group(5 ℃)and the control group(25 ℃).The results showed that,(1)After domestication,Passer montanus body weight in low-temperature group decreased 0.80 g in total,body mass reached very significant level compared to the control group(p<0.01);body weight in high-temperature group increased 0.60 g in total,very significant level compared to the control group(p<0.01).(2)Basal metabolic rate in high-temperature group in 0～1 weeks got obvious decrease,after four weeks domestication decrease amount of 0.39 mL O2/(g·h),it reached very significant level compared to the control group(p<0.01);basal metabolic rate in low-temperature group in 1～3 weeks got obvious increase,after four weeks domestication increase amount of 0.51 mL O2/(g·h),it reached very significant level compared to the control group(p<0.01).(3)Body fat mass fraction in low-temperature group was lower 3.17% than that in control group,body fat mass fraction in high-temperature group was higher 1.46% than that in control group,the body fat mass fractions of tree sparrows among the low temperature group,the three groups were significantly different(p<0.01).(4)The body debranching enzyme activity in muscle of tree sparrows among the low temperature group,the three groups were no significantly different(p>0.05);low-temperature group debranching enzyme activity in liver was very significant compared to the high-temperature group(p<0.01).(5)Control group muscle glycogen was very significant compared to the low-temperature group and high-temperature group(p<0.01);Three groups liver glycogen was very significant compared to each other(p<0.01).(6)The dry weight of kidney,pancreas and spleen in low-temperature group and high-temperature group were not reached very significant level compared them with the control group(p>0.05).Temperature was the main environmental factors affecting the change of thermogenesis at the condition of the outside temperature.In the high-temperature,Passer montanus could adapt itself to the change of temperature by lowering body weight,basal metabolic rate,debranching enzyme activity in liver and muscle tissues to compensate the energy consumption from a series of physiological and biochemical reactions.
引文
[1]陈晓宇，张淑萍．影响鸟类生殖内分泌的环境因子概述[J]．生物学通报，2011,46(12):3-5
[2] Klaassen M,Oltrogge M,Trost L,et al.Basal metabolic rate,food intake,and body mass in cold-and warm-acclimated Garden Warblers[J].Comparative Biochemistry and Physiology Part A Molecular&Integrative Physiology,2004,137(4):639-647
[3] Gavrilov V M.Energy expenditures for flight,aerodynamic quality,and colonization of forest habitats by birds[J].Biology Bulletin,2011,38(8):779-788
[4] Tieleman B I,Williams J B,Buschur M E,et al.Phenotypic variation of larks along an aridty gradient:are desert birds more flexible[J].Ecology,2003,84(7):1800-1815
[5]徐兴军，王昌河，张伟伟，等．温度对麻雀肝糖原和肌糖原含量的影响[J]．黑龙江畜牧兽医，2013(11):164-166
[6]柳劲松，李铭，邵淑丽．树麻雀肝脏和肌肉产热特征的季节性变化[J]．动物学报，2008,54(5):777-784
[7]徐兴军，邵淑丽，张伟伟，等．不同食物对麻雀肝糖原和肌糖原含量的影响[J]．黑龙江畜牧兽医，2012(23):126-128
[8] Makino Y,Omichi K.Purification of glycogen debranching enzyme from porcine brain:evidence for glycogen catabolism in the brain[J].Bioscience Biotechnology&Biochemistry,2006,70(4):907-915
[9] Duan X,Wu J.Advances in studying microbial GH13 starch debranching enzyme--a review[J].Acta microbiologica Sinica,2013,53(7):648-656
[10] Montgomery M K,Hulbert A J,Buttemer W A,et al.Metabolic rate and membrane fatty acid composition in birds:a comparison between long-living parrots and short-living fowl[J].Journal of Comparative Physiology B,2012,182(1):127-137
[11] Nilsson J F,Nilsson J.Fluctuating selection on basal metabolic rate[J].Ecology&Evolution,2016,6(4):1197-1202
[12]王有祥，徐兴军，邵淑丽，等．光周期对白腰朱顶雀脱支酶活性的影响[J]．湖南农业大学学报：自然科学版，2016,42(5):518-523
[13] Vézina F,Dekinga A,Piersma T,et al.Shorebirdsa€TM Seasonal Adjustments in Thermogenic Capacity Are Reflected by Changes in Body Mass:How Preprogrammed and Instantaneous Acclimation Work Together[J].Integrative&Comparative Biology,2011,51(3):394
[14] Gomes F S,Anjos L A,Vasconcellos M T,et al.Influence of different body mass index cut-off values in assessing the nutritional status of adolescents in a household survey[J].Cadernos De Saúde Pública,2009,25(8):1850
[15]王有祥，徐兴军，邵淑丽，等．温度驯化对白腰朱顶雀脱支酶活性的影响[J]．东北林业大学学报，2015(9):107-110
[16] Chavarría-Aguilar L M,García-Herrera R A,Salazar-Cuytun R,et al.Relationship between body fat depots and body mass index in Pelibuey ewes[J].Small Ruminant Research,2016,141:124-126
[17] Dubois K,Hallot F,Vezina F,et al.Basal and maximal metabolic rates differ in their response to rapid temperature change among avian species[J].Journal of Comparative Physiology B,2016,186(7):1-17
[18] Nespolo R F,Bacigalupe L D,Rezende E L,et al.When nonshivering thermogenesis equals maximum metabolic rate:thermal acclimation and phenotypic plasticity of fossorial Spalacopus cyanus(Rodentia)[J].Physiological&Biochemical Zoology,2001,74(3):325-332
[19] Liknes E T,Swanson D L.Seasonal variation in cold tolerance,basal metabolic rate,and maximal capacity for thermogenesis in white-breasted nuthatches,Sitta carolinensis and downy woodpeckers Picoides pubescens,two unrelated arboreal temperate residents[J].Avian Biol,1996,27:279-288
[20] O'connor T P.Metabolic characteristics and body composition in house finches:effects of seasonal acclimatization[J].Comp Physiol,1995(165B):298-305
[21]杨志宏，邵淑丽．食物质量差异对树麻雀能量预算和消化道形态特征的影响[J]．生态学报，2011,31(14):3937-3946
[22] Wang D H,Yang M,Liu Q S,et al.Physiological ecology in small mammals and evolutionary theory[J].Acta Theriol Sin,2009,29(4):343-351
[23] Cooper C E,Withers P C.Effects of measurement duration on the determination of basal metabolic rate and evaporative water loss of small marsupials:how long is long enough[J].Physiological&Biochemical Zoology Pbz,2009,82(5):438-46
[24] Bai M,Wu X,Cai K,et al.Relationships between interspecific differences in the mass of internal organs,biochemical markers of metabolic activity,and the thermogenic properties of three small passerines[J].Avian Research,2016,7(1):11
[25] Nilsson J F,Nilsson J.Fluctuating selection on basal metabolic rate[J].Ecology&Evolution,2016,6(4):1197-1202
[26] Liu J S,Sun R Y,Wang D H,et al.Thermogenic properties in three rodent species from Northeastern China in summer[J].Journal of Thermal Biology,2006,31(1-2):172-176
[27] LI Ming,YIN Yajie,NIE Chunyu,et al.Relationship Between Organ Masses and Basal Metabolic Rate(BMR)in Tree Sparrows(Passer montanus)[J].Journal of Northeast Agricultural University,2011,18(4):39-49
[28]刘丽，田建云，李琦华，等．解偶联蛋白与动物的能量代谢[J]．云南农业大学学报，2006,21(4):504-510
[29] Yan Y, Xie X. Liver mitochondrial and whole-animal level metabolic compensation in a catfish during seasonal acclimatization[J].Current Zoology,2011,57(1):109-115
[30] Huttemann M,Arnold S,Lee I,et al.Turkey cytochrome c oxidase contains subunit VIa of the liver type associated with low efficiency of energy transduction[J].Eur J Biochem,2000,267:2098-2104
[31]林琳，曹梦婷，胡益林，等．环境温度对白头鹎代谢产热和蒸发失水的影响[J]．生态学报，2014,34(3):564-571
[32] Zhai L,Feng L,Xia L,et al.Crystal structure of glycogen debranching enzyme and insights into its catalysis and disease-causing mutations[J].Nature Communications,2016(7):11229
[33] Wang W,We M,Song H M,et al.Diagnosis of glycogen storage disease type IIIA by detecting glycogen debranching enzyme activity,glycogen content and structure in muscle[J].Zhonghua Er Ke Za Zhi Chinese Journal of Pediatrics,2009,47(8):608-612
[34] Adeva-Andany M M,Perez-Felpete N,Fernandez-Fernandez C,et al.Liver glucose metabolism in humans[J].Bioscience reports,2016,36(6):e00416
[35] Gavin J P,Myers S D,Willems M E T,et al.The effect of glycogen reduction on cardiorespiratory and metabolic responses during downhill running[J].European Journal of Applied Physiology,2015,115(5):1125-1133
[36] Froese D S,Michael A,MccorvieT J,et al.Structural basis of glycogen branching enzyme deficiency and pharmacologic rescue by rational peptide design[J].Human Molecular Genetics,2015,2015(20):1-10
[37]冯照军，季丽萍，施雯，等．中华蟾蜍糖原含量的季节变化[J]．动物学报，2007,53(6):1048-1053
[38]徐兴军，邵淑丽，张伟伟，等．饥饿条件下麻雀体内糖原含量和基础代谢率的变化[J]．江苏农业科学，2012,40(11):348-350
[39] Rathnamma V V,Kumar M V,Philip G H.Effect of Deltamethrin on glycogen phosphorylase and glucose-6-phosphatase activity in freshwater fish Labeo rohita[J].Bulletin of Pure&Applied Sciences-Zoology,2007,26(2):1
[40]阴文娅，曾果，张彩云．小鼠肝糖原测定影响因素分析[J]．现代预防医学，2004,31(3):359-360
[2] Klaassen M,Oltrogge M,Trost L,et al.Basal metabolic rate,food intake,and body mass in cold-and warm-acclimated Garden Warblers[J].Comparative Biochemistry and Physiology Part A Molecular&Integrative Physiology,2004,137(4):639-647
[3] Gavrilov V M.Energy expenditures for flight,aerodynamic quality,and colonization of forest habitats by birds[J].Biology Bulletin,2011,38(8):779-788
[4] Tieleman B I,Williams J B,Buschur M E,et al.Phenotypic variation of larks along an aridty gradient:are desert birds more flexible[J].Ecology,2003,84(7):1800-1815
[5]徐兴军，王昌河，张伟伟，等．温度对麻雀肝糖原和肌糖原含量的影响[J]．黑龙江畜牧兽医，2013(11):164-166
[6]柳劲松，李铭，邵淑丽．树麻雀肝脏和肌肉产热特征的季节性变化[J]．动物学报，2008,54(5):777-784
[7]徐兴军，邵淑丽，张伟伟，等．不同食物对麻雀肝糖原和肌糖原含量的影响[J]．黑龙江畜牧兽医，2012(23):126-128
[8] Makino Y,Omichi K.Purification of glycogen debranching enzyme from porcine brain:evidence for glycogen catabolism in the brain[J].Bioscience Biotechnology&Biochemistry,2006,70(4):907-915
[9] Duan X,Wu J.Advances in studying microbial GH13 starch debranching enzyme--a review[J].Acta microbiologica Sinica,2013,53(7):648-656
[10] Montgomery M K,Hulbert A J,Buttemer W A,et al.Metabolic rate and membrane fatty acid composition in birds:a comparison between long-living parrots and short-living fowl[J].Journal of Comparative Physiology B,2012,182(1):127-137
[11] Nilsson J F,Nilsson J.Fluctuating selection on basal metabolic rate[J].Ecology&Evolution,2016,6(4):1197-1202
[12]王有祥，徐兴军，邵淑丽，等．光周期对白腰朱顶雀脱支酶活性的影响[J]．湖南农业大学学报：自然科学版，2016,42(5):518-523
[13] Vézina F,Dekinga A,Piersma T,et al.Shorebirdsa€TM Seasonal Adjustments in Thermogenic Capacity Are Reflected by Changes in Body Mass:How Preprogrammed and Instantaneous Acclimation Work Together[J].Integrative&Comparative Biology,2011,51(3):394
[14] Gomes F S,Anjos L A,Vasconcellos M T,et al.Influence of different body mass index cut-off values in assessing the nutritional status of adolescents in a household survey[J].Cadernos De Saúde Pública,2009,25(8):1850
[15]王有祥，徐兴军，邵淑丽，等．温度驯化对白腰朱顶雀脱支酶活性的影响[J]．东北林业大学学报，2015(9):107-110
[16] Chavarría-Aguilar L M,García-Herrera R A,Salazar-Cuytun R,et al.Relationship between body fat depots and body mass index in Pelibuey ewes[J].Small Ruminant Research,2016,141:124-126
[17] Dubois K,Hallot F,Vezina F,et al.Basal and maximal metabolic rates differ in their response to rapid temperature change among avian species[J].Journal of Comparative Physiology B,2016,186(7):1-17
[18] Nespolo R F,Bacigalupe L D,Rezende E L,et al.When nonshivering thermogenesis equals maximum metabolic rate:thermal acclimation and phenotypic plasticity of fossorial Spalacopus cyanus(Rodentia)[J].Physiological&Biochemical Zoology,2001,74(3):325-332
[19] Liknes E T,Swanson D L.Seasonal variation in cold tolerance,basal metabolic rate,and maximal capacity for thermogenesis in white-breasted nuthatches,Sitta carolinensis and downy woodpeckers Picoides pubescens,two unrelated arboreal temperate residents[J].Avian Biol,1996,27:279-288
[20] O'connor T P.Metabolic characteristics and body composition in house finches:effects of seasonal acclimatization[J].Comp Physiol,1995(165B):298-305
[21]杨志宏，邵淑丽．食物质量差异对树麻雀能量预算和消化道形态特征的影响[J]．生态学报，2011,31(14):3937-3946
[22] Wang D H,Yang M,Liu Q S,et al.Physiological ecology in small mammals and evolutionary theory[J].Acta Theriol Sin,2009,29(4):343-351
[23] Cooper C E,Withers P C.Effects of measurement duration on the determination of basal metabolic rate and evaporative water loss of small marsupials:how long is long enough[J].Physiological&Biochemical Zoology Pbz,2009,82(5):438-46
[24] Bai M,Wu X,Cai K,et al.Relationships between interspecific differences in the mass of internal organs,biochemical markers of metabolic activity,and the thermogenic properties of three small passerines[J].Avian Research,2016,7(1):11
[25] Nilsson J F,Nilsson J.Fluctuating selection on basal metabolic rate[J].Ecology&Evolution,2016,6(4):1197-1202
[26] Liu J S,Sun R Y,Wang D H,et al.Thermogenic properties in three rodent species from Northeastern China in summer[J].Journal of Thermal Biology,2006,31(1-2):172-176
[27] LI Ming,YIN Yajie,NIE Chunyu,et al.Relationship Between Organ Masses and Basal Metabolic Rate(BMR)in Tree Sparrows(Passer montanus)[J].Journal of Northeast Agricultural University,2011,18(4):39-49
[28]刘丽，田建云，李琦华，等．解偶联蛋白与动物的能量代谢[J]．云南农业大学学报，2006,21(4):504-510
[29] Yan Y, Xie X. Liver mitochondrial and whole-animal level metabolic compensation in a catfish during seasonal acclimatization[J].Current Zoology,2011,57(1):109-115
[30] Huttemann M,Arnold S,Lee I,et al.Turkey cytochrome c oxidase contains subunit VIa of the liver type associated with low efficiency of energy transduction[J].Eur J Biochem,2000,267:2098-2104
[31]林琳，曹梦婷，胡益林，等．环境温度对白头鹎代谢产热和蒸发失水的影响[J]．生态学报，2014,34(3):564-571
[32] Zhai L,Feng L,Xia L,et al.Crystal structure of glycogen debranching enzyme and insights into its catalysis and disease-causing mutations[J].Nature Communications,2016(7):11229
[33] Wang W,We M,Song H M,et al.Diagnosis of glycogen storage disease type IIIA by detecting glycogen debranching enzyme activity,glycogen content and structure in muscle[J].Zhonghua Er Ke Za Zhi Chinese Journal of Pediatrics,2009,47(8):608-612
[34] Adeva-Andany M M,Perez-Felpete N,Fernandez-Fernandez C,et al.Liver glucose metabolism in humans[J].Bioscience reports,2016,36(6):e00416
[35] Gavin J P,Myers S D,Willems M E T,et al.The effect of glycogen reduction on cardiorespiratory and metabolic responses during downhill running[J].European Journal of Applied Physiology,2015,115(5):1125-1133
[36] Froese D S,Michael A,MccorvieT J,et al.Structural basis of glycogen branching enzyme deficiency and pharmacologic rescue by rational peptide design[J].Human Molecular Genetics,2015,2015(20):1-10
[37]冯照军，季丽萍，施雯，等．中华蟾蜍糖原含量的季节变化[J]．动物学报，2007,53(6):1048-1053
[38]徐兴军，邵淑丽，张伟伟，等．饥饿条件下麻雀体内糖原含量和基础代谢率的变化[J]．江苏农业科学，2012,40(11):348-350
[39] Rathnamma V V,Kumar M V,Philip G H.Effect of Deltamethrin on glycogen phosphorylase and glucose-6-phosphatase activity in freshwater fish Labeo rohita[J].Bulletin of Pure&Applied Sciences-Zoology,2007,26(2):1
[40]阴文娅，曾果，张彩云．小鼠肝糖原测定影响因素分析[J]．现代预防医学，2004,31(3):359-360