中低温条件下牛粪秸秆混合沼气发酵的研究
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摘要
文章以海林农场为实验基地,通过静态实验的方式以玉米秸秆和牛粪为原料,优化发酵底物碳氮比(C/N)、发酵温度和总固体质量浓度(TS),通过响应面优化,得到最佳的中低温环境下厌氧发酵条件。在混合底物发酵过程中,得到中低温环境下发酵的最佳C/N为25:1。在温度和TS的影响试验中得到中温环境下,产甲烷效率会受到较高TS的影响,最高累计产气量在35℃,TS为15%时获得,为14 030.95 mL。最终通过响应面优化得到符合海林农场的最佳产气条件为温度25℃,TS为17.6%,累计产气量为8 665.5 mL。结果表明,在北方地区,通过优化C/N、温度以及TS,仍然可以获得较好的厌氧发酵效果。
The C/N ratio of fermentation substrate,fermentation temperature and total solid mass concentration(TS)were optimized in the experimental base of Hailin Farm when corn straw and cow dung were used as raw materials.The optimal anaerobic fermentation conditions were obtained by response surface optimization.In the process of mixed substrate fermentation,the optimum C/N ratio for medium and low temperature fermentation was 25∶1.The methane production efficiency was affected by a higher TS in the medium temperature environment.The maximum cumulative gas production was obtained at 35 ℃and 15% TS,which was 14 030.95 mL.Finally,the optimum gas production conditions were that a temperature was 25 ℃,TS was 17.6%,and cumulative gas production was 8665.5 mL by use of response surface optimization.The results showed that a better anaerobic fermentation could still be achieved by optimizing C/N,temperature and TS in the northern region.
The C/N ratio of fermentation substrate,fermentation temperature and total solid mass concentration(TS)were optimized in the experimental base of Hailin Farm when corn straw and cow dung were used as raw materials.The optimal anaerobic fermentation conditions were obtained by response surface optimization.In the process of mixed substrate fermentation,the optimum C/N ratio for medium and low temperature fermentation was 25∶1.The methane production efficiency was affected by a higher TS in the medium temperature environment.The maximum cumulative gas production was obtained at 35 ℃and 15% TS,which was 14 030.95 mL.Finally,the optimum gas production conditions were that a temperature was 25 ℃,TS was 17.6%,and cumulative gas production was 8665.5 mL by use of response surface optimization.The results showed that a better anaerobic fermentation could still be achieved by optimizing C/N,temperature and TS in the northern region.
引文
[1]朱颢,胡启春,汤晓玉,等.我国农作物秸秆资源燃料化利用开发进展[J].中国沼气,2017,35(2):115-120.
[2]国家发展和改革委员会.中国资源综合利用年度报告(2014)[EB/OL].(2014-10-09)[2019-02-25].http://www.ndrc.gov.cn/xwzx/xwfb/201410/W020141009609573303019.pdf.
[3]国家发展和改革委员会,国家农业部.我国主要农作物秸秆综合利用率超过80%[EB/OL].(2016-05-27)[2019-02-22].http://hzs.ndrc.gov.cn/zhly/201605/t20160527_805004.html.
[4]国家发展和改革委员会.中国资源综合利用年度报告(2012)[EB/OL].(2013-04-12)[2019-02-25].http://hzs.ndrc.gov.cn/newzwxx/201304/t20130412_537356.html.
[5]黑龙江省统计局.2018黑龙江省统计年鉴[M].北京:中国统计出版社,2018.
[6]HENDRIKS A.T.W.M,ZEEMAN G.Pretreatments to enhance the digestibility of lignocellulosic biomass[J].Bioresource Technology,2009,100(1):10-18.
[7]SEPP?L? M,PYYKK?NEN V,V?IS?NEN A,et al.Biomethane production from maize and liquid cow manure – Effect of share of maize,post-methanation potential and digestate characteristics[J].Fuel,2013,107(9):209-216.
[8]LEHTOM?KI A.,HUTTUNEN S.,RINTALA J.A.Laboratory investigations on co-digestion of energy crops and crop residues with cow manure for methane production:Effect of crop to manure ratio[J].Resources Conservation & Recycling,2007,51(3):591-609.
[9]张彤,李伟,李文静,等.粪秆结构配比厌氧发酵中pH、VFA与产气效果的关系[J].农业环境科学学报,2010,29(12):2425-2430.
[10]HASSIB B,BOUTHEINA R,HANA G,et al.Mesophilic and thermophilic anaerobic co-digestion of abattoir wastewater and fruit and vegetable waste in anaerobic sequencing batch reactors[J].Biodegradation,2009,20(3):401-409.
[11]XIE S,LAWLOR P G,FROST J P,et al.Effect of pig manure to grass silage ratio on methane production in batch anaerobic co-digestion of concentrated pig manure and grass silage[J].Bioresource Technology,2011,102(10):5728-5733.
[12]OTHONG S,BOE K,ANGELIDAKI I.Thermophilic anaerobic co-digestion of oil palm empty fruit bunches with palm oil mill effluent for efficient biogas production[J].Applied Energy,2012,93(5):648-654.
[13]PANG Y.Z,LIU Y.P,LI X.J,et al.Improving biodegradability and biogas production of corn stover through sodium hydroxide solid state pretreatment[J].Energy & Fuels,2008,22(4):2761-2766.
[14]LI H,TAN F,KE L,et al.Mass balances and distributions of C,N,and P in the anaerobic digestion of different substrates and relationships between products and substrates[J].Chemical Engineering Journal,2016,287:329-336.
[15]NING J,ZHOU M,PAN X,et al.Simultaneous biogas and biogas slurry production from co-digestion of pig manure and corn straw:Performance optimization and microbial community shift[J].Bioresourse Technology,2019,282:37-47.
[16]ZHANG Z,ZHANG G Y,LI W L,et al.Enhanced biogas production from sorghum stem by co-digestion with cow manure[J].International Journal of Hydrogen Energy,2016,41(21):9153-9158.
[17]BOUALLAGUI H,LAHDHEB H,ROMDAN E.B,et al.Improvement of fruit and vegetable waste anaerobic digestion performance and stability with co-substrates addition[J].Journal of Environmental Management,2009,90(5):1844-1849.
[18]WANG X,YANG G,FENG Y,et al.Optimizing feeding composition and carbon-nitrogen ratios for improved methane yield during anaerobic co-digestion of dairy,chicken manure and wheat straw[J].Bioresource Technology,2012,120:78-83.
[19]ABBASSI G A,BROCKMANN D,TRABLY E,et al.Total solids content drives high solid anaerobic digestion via mass transfer limitation[J].Bioresource Technology,2012,111:55-61.
[2]国家发展和改革委员会.中国资源综合利用年度报告(2014)[EB/OL].(2014-10-09)[2019-02-25].http://www.ndrc.gov.cn/xwzx/xwfb/201410/W020141009609573303019.pdf.
[3]国家发展和改革委员会,国家农业部.我国主要农作物秸秆综合利用率超过80%[EB/OL].(2016-05-27)[2019-02-22].http://hzs.ndrc.gov.cn/zhly/201605/t20160527_805004.html.
[4]国家发展和改革委员会.中国资源综合利用年度报告(2012)[EB/OL].(2013-04-12)[2019-02-25].http://hzs.ndrc.gov.cn/newzwxx/201304/t20130412_537356.html.
[5]黑龙江省统计局.2018黑龙江省统计年鉴[M].北京:中国统计出版社,2018.
[6]HENDRIKS A.T.W.M,ZEEMAN G.Pretreatments to enhance the digestibility of lignocellulosic biomass[J].Bioresource Technology,2009,100(1):10-18.
[7]SEPP?L? M,PYYKK?NEN V,V?IS?NEN A,et al.Biomethane production from maize and liquid cow manure – Effect of share of maize,post-methanation potential and digestate characteristics[J].Fuel,2013,107(9):209-216.
[8]LEHTOM?KI A.,HUTTUNEN S.,RINTALA J.A.Laboratory investigations on co-digestion of energy crops and crop residues with cow manure for methane production:Effect of crop to manure ratio[J].Resources Conservation & Recycling,2007,51(3):591-609.
[9]张彤,李伟,李文静,等.粪秆结构配比厌氧发酵中pH、VFA与产气效果的关系[J].农业环境科学学报,2010,29(12):2425-2430.
[10]HASSIB B,BOUTHEINA R,HANA G,et al.Mesophilic and thermophilic anaerobic co-digestion of abattoir wastewater and fruit and vegetable waste in anaerobic sequencing batch reactors[J].Biodegradation,2009,20(3):401-409.
[11]XIE S,LAWLOR P G,FROST J P,et al.Effect of pig manure to grass silage ratio on methane production in batch anaerobic co-digestion of concentrated pig manure and grass silage[J].Bioresource Technology,2011,102(10):5728-5733.
[12]OTHONG S,BOE K,ANGELIDAKI I.Thermophilic anaerobic co-digestion of oil palm empty fruit bunches with palm oil mill effluent for efficient biogas production[J].Applied Energy,2012,93(5):648-654.
[13]PANG Y.Z,LIU Y.P,LI X.J,et al.Improving biodegradability and biogas production of corn stover through sodium hydroxide solid state pretreatment[J].Energy & Fuels,2008,22(4):2761-2766.
[14]LI H,TAN F,KE L,et al.Mass balances and distributions of C,N,and P in the anaerobic digestion of different substrates and relationships between products and substrates[J].Chemical Engineering Journal,2016,287:329-336.
[15]NING J,ZHOU M,PAN X,et al.Simultaneous biogas and biogas slurry production from co-digestion of pig manure and corn straw:Performance optimization and microbial community shift[J].Bioresourse Technology,2019,282:37-47.
[16]ZHANG Z,ZHANG G Y,LI W L,et al.Enhanced biogas production from sorghum stem by co-digestion with cow manure[J].International Journal of Hydrogen Energy,2016,41(21):9153-9158.
[17]BOUALLAGUI H,LAHDHEB H,ROMDAN E.B,et al.Improvement of fruit and vegetable waste anaerobic digestion performance and stability with co-substrates addition[J].Journal of Environmental Management,2009,90(5):1844-1849.
[18]WANG X,YANG G,FENG Y,et al.Optimizing feeding composition and carbon-nitrogen ratios for improved methane yield during anaerobic co-digestion of dairy,chicken manure and wheat straw[J].Bioresource Technology,2012,120:78-83.
[19]ABBASSI G A,BROCKMANN D,TRABLY E,et al.Total solids content drives high solid anaerobic digestion via mass transfer limitation[J].Bioresource Technology,2012,111:55-61.