海河流域溶藻菌Q1的溶藻效果分析及鉴定
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摘要
从天津市海河富营养化的水域中分离到1株具有溶藻能力的细菌,将其命名为Q1,对该菌株进行生理生化鉴定及提取16SrRNA分子鉴定,将Q1以10%接种量接入铜绿微囊藻(Microcysis aeruginosa)、水华鱼腥藻(Anabaena flosaquae)和斜生栅藻(Scenedesmus obliquus)的纯培养藻液中,研究Q1对不同微藻的专一性及不同生长时期Q1的菌液对铜绿微囊藻生长的影响、作用方式。结果表明:菌株Q1属于苏云金芽孢杆菌属(Bacillus thuringiensis),该菌株稳定期的滤液溶藻效果最佳,菌株Q1能溶解水华鱼腥藻和铜绿微囊藻,对斜生栅藻也有促进作用。该菌株的无菌滤液经高温处理后,对铜绿微囊藻的溶藻效果没有影响,推断菌株Q1分泌的可耐高温的胞外物质。该菌株与藻体细胞相互抑制,存在竞争共栖的生态关系。
An algae-lysing bacterium strain named Q1 was isolated from eutrophic waters in the Haihe river of Tianjin.The biophysics,biochemical and the analysis of its 16 SrRNA sequence were conducted.To investigate the specificity of algicidal activity against Microcysis-aeruginosa,Anabaena flosaquae and Scenedesmus obliquus by bacterial Strain Q1,we added Q1 to monoculture system of these three microalage at the rate of 10%.The strain Q1 from different growth periods were used to study its inhibition and mode of action against Microcysis aeruginosa.The numbers of cell and the photosynthetic pigments content in M.aeruginosa.were inve stigated The results showed that the strain Q1 was identified as Bacillus thuringiensis.Q1 on stable growth phase showed the best alga-lysing performance.The strain could effectively dissolve M.aeruginosaand A.flosaquae and promote the growth of S obliquus.The aseptic filtrate of the strain was not affected by M.aeruginosa when it was treated at high temperature.Therefore,it can be concluded that a high temperature extracellular substance secreted by strain Q1 had the characteristics of algae lysis.The interaction between strain and algal cell was mutually inhibited,and there was an ecological relationship of competition and cohabitation,between strain and algal.
An algae-lysing bacterium strain named Q1 was isolated from eutrophic waters in the Haihe river of Tianjin.The biophysics,biochemical and the analysis of its 16 SrRNA sequence were conducted.To investigate the specificity of algicidal activity against Microcysis-aeruginosa,Anabaena flosaquae and Scenedesmus obliquus by bacterial Strain Q1,we added Q1 to monoculture system of these three microalage at the rate of 10%.The strain Q1 from different growth periods were used to study its inhibition and mode of action against Microcysis aeruginosa.The numbers of cell and the photosynthetic pigments content in M.aeruginosa.were inve stigated The results showed that the strain Q1 was identified as Bacillus thuringiensis.Q1 on stable growth phase showed the best alga-lysing performance.The strain could effectively dissolve M.aeruginosaand A.flosaquae and promote the growth of S obliquus.The aseptic filtrate of the strain was not affected by M.aeruginosa when it was treated at high temperature.Therefore,it can be concluded that a high temperature extracellular substance secreted by strain Q1 had the characteristics of algae lysis.The interaction between strain and algal cell was mutually inhibited,and there was an ecological relationship of competition and cohabitation,between strain and algal.
引文
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[13]王琼,翟建宏,张骞月,等.侧芽孢杆菌的抑藻效应及对养殖水体中蓝藻水华的生态防控[J].生物灾害科学,2013,36(1):61-65.
[14]黄晶晶,汪雪婷,夏赛周,等.溶藻细菌MS7的分类鉴定及其溶藻活性的初步研究[J].海洋与湖沼,2013,44(3):632-637.
[15]黄姿,李春强,于晓玲,等.1株溶藻细菌(Pseudoalteromonas sp.)的分离鉴定及溶藻活性初探[J].海洋技术,2008,27(3):56-60.
[16]ZHANG H J,AN X L,ZHOU Y Y,et al.Effect of oxidative stress induced by Brevibacteriumsp.BS01on a HAB causing species-Alexandrium tamarense[J].PLos ONE,2013,8(5):e63018.
[17]董晓娜,陈泽慧,毛林强,等.太湖土著激浪鱼内脏中溶藻菌R1的筛选及其特性研究[J].工业安全与环保,2018,44(8):69-72.
[18]牛丹丹,郑青松,刘兆普,等.溶藻细菌YZ对铜绿微囊藻的溶藻特性研究[J].中国环境科学,2011,31(2):321-326.
[19]IMAMURA N,MOTOIKE I,SHIMADA N,et al.An efficient screening approach for anti-microcystis compounds based on knowledge of aquatic microbial ecosystem[J].The Journal of Antibiotics,2001,54(7):582-587.
[2]朱叶飞,唐婉莹.1株溶藻真菌的分离鉴定及其作用机理(制)研究[J].广东化工,2016,43(7):3-4.
[3]沈萍,范秀荣,李广武.微生物学实验[M].北京:高等教育出版社,2002:43-46.
[4]刘晶,潘伟斌,秦玉洁,等.2株溶藻细菌的分离鉴定及其溶藻特性[J].环境科学与技术,2007,30(2):17-19,22.
[5]张红,吕富,吕林兰,等.浮游植物叶绿素a含量测定方法的比较及优化[J].海洋科学,2012,36(10):1-4.
[6]汪辉,刘兆普,魏巍,等.1株溶藻菌的分离、鉴定及其溶藻物质的鉴定[J].中国环境科学,2008,28(5):461-465.
[7]邓建明,陶勇,李大平,等.溶藻细菌及其分子生物学研究进展[J].应用与环境生物学报,2009,15(6):895-900.
[8]WANG J X,LUO G Y,XU X Y,et al.Lytic effect of environmental factors on algae-lysing bacteria strain s7by fluorescence spectroscopy[J].Journal of Shanghai Jiaotong University,2012,46(5):780-784.
[9]张晖,何颖,曹驰骋,等.1株巢湖高效溶藻菌的分离鉴定及其溶藻特性研究[J].环境科学与技术,2018,41(7):1-5.
[10]李文利,刘征宇,陆雅雅,等.蜡样芽孢杆菌培养条件的优化及其溶藻方式的研究[J].环境污染与防治,2016,38(8):20-24.
[11]张菊,韦坤逢,李灿灿,等.小麦内生溶藻细菌ZB1的分离鉴定及其溶藻特性[J].西南农业学报,2017,30(5):1068-1073.
[12]张睿,王广军,李志斐,等.枯草芽孢杆菌对铜绿微囊藻抑制效果的研究[J].中国环境科学,2015,36(6):1814-1821.
[13]王琼,翟建宏,张骞月,等.侧芽孢杆菌的抑藻效应及对养殖水体中蓝藻水华的生态防控[J].生物灾害科学,2013,36(1):61-65.
[14]黄晶晶,汪雪婷,夏赛周,等.溶藻细菌MS7的分类鉴定及其溶藻活性的初步研究[J].海洋与湖沼,2013,44(3):632-637.
[15]黄姿,李春强,于晓玲,等.1株溶藻细菌(Pseudoalteromonas sp.)的分离鉴定及溶藻活性初探[J].海洋技术,2008,27(3):56-60.
[16]ZHANG H J,AN X L,ZHOU Y Y,et al.Effect of oxidative stress induced by Brevibacteriumsp.BS01on a HAB causing species-Alexandrium tamarense[J].PLos ONE,2013,8(5):e63018.
[17]董晓娜,陈泽慧,毛林强,等.太湖土著激浪鱼内脏中溶藻菌R1的筛选及其特性研究[J].工业安全与环保,2018,44(8):69-72.
[18]牛丹丹,郑青松,刘兆普,等.溶藻细菌YZ对铜绿微囊藻的溶藻特性研究[J].中国环境科学,2011,31(2):321-326.
[19]IMAMURA N,MOTOIKE I,SHIMADA N,et al.An efficient screening approach for anti-microcystis compounds based on knowledge of aquatic microbial ecosystem[J].The Journal of Antibiotics,2001,54(7):582-587.