GC-MS测定动物组织中五氯酚钠的不确定度评定
|
摘要
目的评定气相色谱-质谱联用法(GC-MS)测定动物组织中五氯酚钠含量的不确定度。方法依据JJF 1059.1-2012《测量不确定度评定与表示》技术规范,对本方法中的各个不确定度来源进行分析和量化,计算相对扩展不确定度。结果通过计算,当五氯酚钠含量为3.68μg/kg时,其相对扩展不确定度为17%(k=2)。结论本方法不确定度主要来源是拟合校准曲线,建议通过增加标准曲线每个浓度的测定次数、提高检验人员的操作规范性和熟练程度等措施来提高检测结果的准确性。
Objective To evaluate the uncertainty in measurement in the determination of sodium pentachlorophenol in animal tissues by GC-MS. Methods According to JJF 1059.1-2012 "Evaluation and expression of uncertainty in measurement",various sources of uncertainty in the method were analyzed and quantified,and the relative expanded uncertainty was also evaluated by the technical regulation. Results The relative expanded uncertainty was 17%(k=2),when the result of measurement for sodium pentachlorophenol was 3.68 μg/kg. Conclusions The main source of uncertainty in the method was the fitting calibration curve. It is suggested that some measures could improve the accuracy of measurment,such as increasing the number of measurements for each concentration of the standard curve,and improving the standardization and proficiency of inspectors.
Objective To evaluate the uncertainty in measurement in the determination of sodium pentachlorophenol in animal tissues by GC-MS. Methods According to JJF 1059.1-2012 "Evaluation and expression of uncertainty in measurement",various sources of uncertainty in the method were analyzed and quantified,and the relative expanded uncertainty was also evaluated by the technical regulation. Results The relative expanded uncertainty was 17%(k=2),when the result of measurement for sodium pentachlorophenol was 3.68 μg/kg. Conclusions The main source of uncertainty in the method was the fitting calibration curve. It is suggested that some measures could improve the accuracy of measurment,such as increasing the number of measurements for each concentration of the standard curve,and improving the standardization and proficiency of inspectors.
引文
[1]杨平华,陈小艳,张新华,等.气相色谱法测定鄱阳湖湿地多种环境介质中五氯酚钠[J].理化检验(化学分册),2018,54(11):1246-1249.
[2]田志梅,马现永,崔艺燕,等.饲料中五氯酚钠溯源监测分析[J].广东畜牧兽医科技,2018,43(5):30-33.
[3]杨静,秦菊,曾波,等.重庆主城3条主要支流的污染状况调查[J].西南大学学报(自然科学版),2018,40(11):160-167.
[4]陈春丽,向洪锐,戴星照,等.中国天然水体中五氯酚的浓度水平、管理及来源分析[J].生态毒理学报,2015,10(6):71-79.
[5]Kondo T,Yamamoto H,Tatarazako N,et al.Bioconcentration factor of relatively low concentrations of chlorophenols in Japanese medaka[J].Chemosphere,2005,61(9):1299-1304.
[6]许晓国.江苏金坛鱼塘水、底泥、鱼体和渔民血中五氯酚暴露水平研究[D].上海:复旦大学,2010:34-43.
[7]钟文珏,张瑜,韩雨薇,等.沉积物中五氯酚对底栖生物的急慢性毒性效应[J].生态毒理学报,2015,10(1):297-304.
[8]Sun W,Jia YL,Ding XS,et al.Combined effects of pentachlorophenol and its byproduct hexachlorobenzene on endocrine and reproduction in zebrafish[J].Chemosphere,2019,220:216-226.
[9]Li EC,Bolser DG,Kroll KJ,et al.Comparative toxicity of three phenolic compounds on the embryo of fathead minnow,Pimephales promelas[J].Aquat Toxicol,2018,201:66-72.
[10]夏勇,徐彩菊,傅剑云,等.五氯酚钠诱导斑马鱼体细胞p53基因突变的克隆测序研究[J].预防医学,2017,29(4):325-333.
[11]Huang XM,Han XM,Huang ZY,et al.Maternal pentachlorophenol exposure induces developmental toxicity mediated by autophagy on pregnancy mice[J].Ecotoxicol Environ Saf,2019,169:829-836.
[12]郭剑秋,郭梦璐,吕沈亮,等.宫内五氯苯酚暴露与1岁婴儿生长发育的关系[J].环境与健康杂志,2017,34(10):869-873.
[13]夏勇,董晓岚,孟真,等.五氯酚钠的发育毒性和遗传毒性研究[J].预防医学,2016,28(11):1081-1090.
[14]蒋鹏,盛南,王建设,等.五氯酚(PCP)对鸡肝癌细胞(LMH)毒性效应的机制研究[J].生态毒理学报,2017,12(3):373-381.
[15]Pietsch C,Hollender J,Dorusch F,et al.Cytotoxic effects of pentachlorophenol(PCP)and its metabolite tetrachlorohydroquinone(TCHQ)on liver cells are modulated by antioxidants[J].Cell Biol Toxicol,2014,30(4):233-252.
[16]Yang LH,Zha JM,Wang ZJ.Pentachlorophenol affected both reproductive and interrenal systems:In silico and in vivo evidence[J].Chemosphere,2017,166:174-183.
[17]Martin TJ,Whalen MM.Exposures to the Environmental Toxicants Pentachlorophenol(PCP)and Dichlorodiphenyltrichloroethane(DDT)Modify Secretion of Interleukin 1-Beta(IL-1β)from Human Immune Cells[J].Arch Toxicol,2017,91(4):1795-1808.
[18]武丽辉.24种农药产品被列入“高污染、高环境风险”产品目录[J].农药科学与管理,2008,29(6):54-55.
[19]国家质量监督检验检疫总局.测量不确定度评定与表示:JJF1059.1-2012[S].北京:中国质检出版社,2013:4-26.
[20]王艳敏,周鸿,刘源.石墨炉原子吸收光谱法测定绿茶中镉的不确定度评定[J].实验与检验医学,2017,35(1):23-27.
[21]国家质量监督检验检疫总局.中华人民共和国国家计量检定规程常用玻璃量器:JJG 196-2006[S].北京:中国计量出版社,2007:6-9.
[2]田志梅,马现永,崔艺燕,等.饲料中五氯酚钠溯源监测分析[J].广东畜牧兽医科技,2018,43(5):30-33.
[3]杨静,秦菊,曾波,等.重庆主城3条主要支流的污染状况调查[J].西南大学学报(自然科学版),2018,40(11):160-167.
[4]陈春丽,向洪锐,戴星照,等.中国天然水体中五氯酚的浓度水平、管理及来源分析[J].生态毒理学报,2015,10(6):71-79.
[5]Kondo T,Yamamoto H,Tatarazako N,et al.Bioconcentration factor of relatively low concentrations of chlorophenols in Japanese medaka[J].Chemosphere,2005,61(9):1299-1304.
[6]许晓国.江苏金坛鱼塘水、底泥、鱼体和渔民血中五氯酚暴露水平研究[D].上海:复旦大学,2010:34-43.
[7]钟文珏,张瑜,韩雨薇,等.沉积物中五氯酚对底栖生物的急慢性毒性效应[J].生态毒理学报,2015,10(1):297-304.
[8]Sun W,Jia YL,Ding XS,et al.Combined effects of pentachlorophenol and its byproduct hexachlorobenzene on endocrine and reproduction in zebrafish[J].Chemosphere,2019,220:216-226.
[9]Li EC,Bolser DG,Kroll KJ,et al.Comparative toxicity of three phenolic compounds on the embryo of fathead minnow,Pimephales promelas[J].Aquat Toxicol,2018,201:66-72.
[10]夏勇,徐彩菊,傅剑云,等.五氯酚钠诱导斑马鱼体细胞p53基因突变的克隆测序研究[J].预防医学,2017,29(4):325-333.
[11]Huang XM,Han XM,Huang ZY,et al.Maternal pentachlorophenol exposure induces developmental toxicity mediated by autophagy on pregnancy mice[J].Ecotoxicol Environ Saf,2019,169:829-836.
[12]郭剑秋,郭梦璐,吕沈亮,等.宫内五氯苯酚暴露与1岁婴儿生长发育的关系[J].环境与健康杂志,2017,34(10):869-873.
[13]夏勇,董晓岚,孟真,等.五氯酚钠的发育毒性和遗传毒性研究[J].预防医学,2016,28(11):1081-1090.
[14]蒋鹏,盛南,王建设,等.五氯酚(PCP)对鸡肝癌细胞(LMH)毒性效应的机制研究[J].生态毒理学报,2017,12(3):373-381.
[15]Pietsch C,Hollender J,Dorusch F,et al.Cytotoxic effects of pentachlorophenol(PCP)and its metabolite tetrachlorohydroquinone(TCHQ)on liver cells are modulated by antioxidants[J].Cell Biol Toxicol,2014,30(4):233-252.
[16]Yang LH,Zha JM,Wang ZJ.Pentachlorophenol affected both reproductive and interrenal systems:In silico and in vivo evidence[J].Chemosphere,2017,166:174-183.
[17]Martin TJ,Whalen MM.Exposures to the Environmental Toxicants Pentachlorophenol(PCP)and Dichlorodiphenyltrichloroethane(DDT)Modify Secretion of Interleukin 1-Beta(IL-1β)from Human Immune Cells[J].Arch Toxicol,2017,91(4):1795-1808.
[18]武丽辉.24种农药产品被列入“高污染、高环境风险”产品目录[J].农药科学与管理,2008,29(6):54-55.
[19]国家质量监督检验检疫总局.测量不确定度评定与表示:JJF1059.1-2012[S].北京:中国质检出版社,2013:4-26.
[20]王艳敏,周鸿,刘源.石墨炉原子吸收光谱法测定绿茶中镉的不确定度评定[J].实验与检验医学,2017,35(1):23-27.
[21]国家质量监督检验检疫总局.中华人民共和国国家计量检定规程常用玻璃量器:JJG 196-2006[S].北京:中国计量出版社,2007:6-9.