彩叶芋组织快速繁殖技术研究
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摘要
【目的】研究最适宜彩叶芋组织快速繁殖的激素浓度,为其工厂化生产提供借鉴和帮助。【方法】以漆斑彩叶芋和红艳彩叶芋为材料,以MS为基本培养基,添加不同浓度的6-BA和NAA进行组培繁殖,对幼叶外植体的愈伤组织诱导以及愈伤组织增殖分化体系进行优化。【结果】在不同浓度范围内,不同的植物生长调节剂组合对愈伤组织诱导、增殖分化有不同影响,但均能促使彩叶芋产生愈伤组织,再生出完整植株。炼苗后移栽至由泥炭土、蛭石和珍珠岩混合的基质中,瓶苗的成活率可达90%以上。【结论】MS+6-BA 4.0 mg/L+NAA 0.5 mg/L是适合彩叶芋愈伤组织诱导的最佳培养基,启动时间短,产愈率高;MS+6-BA 2.0 mg/L+NAA 0.2mg/L是适合彩叶芋分化增殖的最佳培养基,愈伤组织分化率最高,试管苗的生长情况最好。
【Objective】The research aimed to study the optimal hormone concentration for the rapid propagation of caladium bicolor tissue, and to provide reference and help for its factory production.【Method】Young leaves developing from C. hortulanum ‘Wightii' and C. hortulanum ‘Postman Joyner' were used as the explant to make an optimization experiment of callus induction and callus proliferation differentiation system, by taking tissue culture and propagation in different formulations of 6-BA and NAA on MS basal medium. 【Result】Different combinations of plant growth regulators showed different effects on callus induction, differentiation and proliferation within a certain mass concentration. All the C. bicolors could induce callus and regenerated plants in these different formulations. After transplanting to the matrix mixed with peat soil, vermiculite and perlite, the survival rate of seedling was more than 90%.【Conclusion】The results showed that MS+6-BA 4.0 mg/L+NAA 0.5 mg/L was the best culture medium for callus induction of C. bicolor with short start-up time and high cure rate, while MS+6-BA 2.0 mg/L+NAA 0.2 mg/L was most effective for differentiation and proliferation with the highest callus differentiation rate and the best growth of test-tube seedlings.
【Objective】The research aimed to study the optimal hormone concentration for the rapid propagation of caladium bicolor tissue, and to provide reference and help for its factory production.【Method】Young leaves developing from C. hortulanum ‘Wightii' and C. hortulanum ‘Postman Joyner' were used as the explant to make an optimization experiment of callus induction and callus proliferation differentiation system, by taking tissue culture and propagation in different formulations of 6-BA and NAA on MS basal medium. 【Result】Different combinations of plant growth regulators showed different effects on callus induction, differentiation and proliferation within a certain mass concentration. All the C. bicolors could induce callus and regenerated plants in these different formulations. After transplanting to the matrix mixed with peat soil, vermiculite and perlite, the survival rate of seedling was more than 90%.【Conclusion】The results showed that MS+6-BA 4.0 mg/L+NAA 0.5 mg/L was the best culture medium for callus induction of C. bicolor with short start-up time and high cure rate, while MS+6-BA 2.0 mg/L+NAA 0.2 mg/L was most effective for differentiation and proliferation with the highest callus differentiation rate and the best growth of test-tube seedlings.
引文
[1]CAI X D,CAO Z,XU S X,DENG Z N.Induction,regeneration and characterization of tetraploids and variants in‘Tapestry’Caladium[J].Plant Cell Tissue&Organ Culture,2015,120(2):689-700.
[2]韩清华.彩叶芋的品种及栽培管理[J].中国花卉园艺,2007(12):34-35.HAN Q H.Varieties and cultivation management of Caladium bicolor[J].China Flowers&Horticulture,2007(12):34-35.
[3]BALDWIN D,MIRANDA G J,WILFRET B K,HARBAUGH.‘Florida White Ruffles’-A white strap-leaved Caladium for small pots,hanging baskets,or sunny landscapes[J].Hort Science,2002,5:838-840.
[4]周肇基.七彩缤纷彩叶芋[J].花木盆景(花卉园艺),2009(3):16-17.ZHOU Z J.Colorful Caladium bicolor[J].Flower Plant&Penjing,2009(3):16-17.
[5]周肇基.观叶新宠彩叶芋[J].花木盆景(花卉园艺),2000(12):4.ZHOU Z J.New favorite of foliage Caladium bicolor[J].Flower Plant&Penjing,2000(12):4
[6]周默.彩叶植物新品[J].中国花卉园艺,2016(6):56.ZHOU M.Colorful leaf plant[J].China Flowers&Horticulture,2016(6):56.
[7]蔡明.花叶芋组培快繁技术[J].内蒙古林业,1999(9):28.CAI M.Tissue culture and rapid propagation of Caladium bicolor[J].Inner Mongolia Forestry,1999(9):28.
[8]李国义,龚束芳,张丽梅,朱祥春,车代弟.彩色马蹄莲组培快繁技术的研究[J].北方园艺,2004(2):64-65.LI G Y,GONG S F,ZHANG L M,ZHU X C,CHE D D.Study on tissue culture and rapid propagation of Zantedeschia hybida[J].Northern Horticulture,2004(2):64-65.
[9]张东旭,周增产,卜云龙,张洁,张晓文,张成波,蔡伟健,康静,肖政.植物组织培养技术应用研究进展[J].北方园艺,2011(6):209-213.ZHANG D X,ZHOU Z C,BU Y L,ZHANG J,ZHANG X W,ZHANGC B,CAI W J,KANG J,XIAO Z.Research progress on plant tissue culture technology[J].Northern Horticulture,2011(6):209-213.
[10]PENCE,VALERIE C.Evaluating costs for the in vitro propagation and preservation of endangered plants[J].In Vitro Cellular&Developmental Biology,2011,47(1):176-187.
[11]黄祖传.绚丽多彩的彩叶芋[J].中国花卉盆景,1990(11):11.HUANG Z C.Bright and colourful Caladium bicolor[J].China Flower&Penjing,1990(11):11.
[12]董少鸣.彩叶芋的组织培养及快速繁殖[J].承德民族师专学报,2003,23(2):76.doi:10.16729/j.cnki.jhnun.2003.02.039.DONG S M.The histocultivating of colored-leaves taros and its rapid reproduction[J].Journal of Chengde Teachers'College for Nationalities,2003,23(2):76.doi:10.16729/j.cnki.jhnun.2003.02.039.
[13]王颖,陆国权.彩叶芋组织培养研究进展[J].北方园艺,2012(12):196-198.WANG Y,LU G Q.Research advances of Caladium bicolor[J].Northern Horticulture,2012(12):196-198.
[14]吴蓓,戴修纯,郑岩松,张木清,李光光,陈胜文,刘绍钦.大田苦瓜的离体快繁研究[J].广东农业科学,2017,44(12):27-32.doi:10.16768/j.issn.1004-874X.2017.12.005.WU B,DAI X C,ZHENG Y S,ZHANG M Q,LI G G,CHEN S W,LIUS Q.In vitro propagation of bitter gourd(Momordica charantia Linn.)for field culti-vation[J].Guangdong Agricultural Sciences,2017,44(12):27-32.doi:10.16768/j.issn.1004-874X.2017.12.005.
[15]高燕会,童再康,黄华宏,郭福起,郁重彦.花叶络石的组织培养[J].浙江农林大学学报,2006,23(6):701-704.GAO Y H,TONG Z K,HUANG H H,GUO F Q,YU C Y.Tissue culture on Trachelospermum jasminoides`Variegatum'[J].Journal of Zhejiang A&F University,2006,23(6):701-704.
[16]陈月桂,谭嘉娜,官锦燕,罗剑飘,黄海英,罗青文,杨俊贤,李奇伟.甘蔗新品种粤糖03-373组培快繁试验[J].广东农业科学,2017,44(5):13-18.doi:10.16768/j.issn.1004-874X.2017.05.003.CHEN Y G,TAN J N,GUAN J Y,LUO J P,HUANG H Y,LUOQ W,YANG J X,LI Q W.Tissue culture and rapid propagation of Yuetang 03-373,a new sugarcane variety[J].Guangdong Agricultural Sciences,2017,44(5):13-18.doi:10.16768/j.issn.1004-874X.2017.05.003.
[17]吴满良,李冬梅,朱根发,刘小飞.紫花山奈的组培快繁研究[J].广东农业科学,2018,45(7):29-33.doi:10.16768/j.issn.1004-874X.2018.07.005.WU M L,LI D M,ZHU G F,LIU X F.In vitro rapid propagation of Kaempferia elegans[J].Guangdong Agricultural Sciences,2018,45(7):29-33.doi:10.16768/j.issn.1004-874X.2018.07.005.
[18]蔡小东,DENG Z N.彩叶芋愈伤组织的诱导、增殖及植株再生[J].江苏农业科学,2016,44(9):75-77.doi:10.15889/j.issn.1002-1302.2016.09.021.CAI X D,DENG Z N.Callus induction,proliferation and plantlet regeneration of Caladium bicolor[J].Jiangsu Agricultural Sciences,2016,44(9):75-77.doi:10.15889/j.issn.1002-1302.2016.09.021.
[19]李维强,刘静.花叶芋组培快繁的应用研究[J].郑州牧业工程高等专科学校学报,2004(4):260-261.LI W Q,LIU J.Study on the technology of Caladium bicolor tissue culture and rapid multiplication[J].Journal of Zhengzhou College of Animal Husbandry&Engineering,2004(4):260-261.
[20]祝骥,姚汝华,黄毓文,梁承邺.苦丁茶愈伤组织的诱导与褐变抑制[J].热带亚热带植物学报,2000,8(4):319-323.ZHU J,YAO R H,HUANG Y W,LIANG C Y.Callus induction and browning inhibition in Ilex kudingcha C.J.TSENG[J].Journal of Tropical and Subtropical Botany,2000,8(4):319-323.
[21]罗阳春,杨云,李仕伟,方芳,王鹏蒲,全波,郑祖平.组织培养中植物外植体及愈伤组织褐变的研究进展[J].贵州农业科学,2018,46(1):5-10.LUO Y C,YANG Y,LI S W,FANG F,WANG P P,QUAN B,ZHENG ZP.Research progress on browning of explant and callus in plant tissue culture[J].Guizhou Agricultural Sciences,2018,46(1):5-10.
[22]BROOME O C,ZIMMERMAN R H.In vitro propagation of blackberry[J].Hortieulture Science,1978,13:151-153.
[23]肖小君,罗潼,王芳.木本植物组织培养过程中褐变现象及控制措施的研究进展[J].江苏农业科学,2017,45(16):20-24.doi:10.15889/j.issn.1002-1302.2017.16.004.XIAO X J,LUO T,WANG F.Research process on browning and control measures of woody plants tissue culture[J].Jiangsu Agricultural Sciences,2017,45(16):20-24.doi:10.19415/j.cnki.1673-890x.2015.36.009.
[24]任辉丽.植物组培技术常见问题及其预防措施[J].南方农业,2015,36(9):20-21.doi:10.19415/j.cnki.1673-890x.2015.36.009.REN H L.Common problems and preventive measures of plant tissue culture technology[J].South China Agriculture,2015,36(9):20-21.doi:10.19415/j.cnki.1673-890x.2015.36.009.
[25]姚丽娟,徐晓薇,林绍生,陈中林,陈义增.洋兰组培快繁褐变抑制因子探讨[J].北方园艺,2006(4):162-163.YAO L J,XU X W,LING S S,CHEN Z L,CHEN Y Z.Inhibitor of browning in tissue culture of orchid[J].Northern Horticulture,2006(4):162-163.
[26]贾荟芹,高龙梅,李薇,杨小丽,胡振兴.黄花菜组织培养褐变研究[J].农业开发与装备,2015(8):69.JIA H Q,GAO L M,LI W,YANG X L,HU Z X.Study on browning in tissue culture of Hemerocallis citrine Baroni[J].Agricultural Development&Equipments,2015(8):69.
[2]韩清华.彩叶芋的品种及栽培管理[J].中国花卉园艺,2007(12):34-35.HAN Q H.Varieties and cultivation management of Caladium bicolor[J].China Flowers&Horticulture,2007(12):34-35.
[3]BALDWIN D,MIRANDA G J,WILFRET B K,HARBAUGH.‘Florida White Ruffles’-A white strap-leaved Caladium for small pots,hanging baskets,or sunny landscapes[J].Hort Science,2002,5:838-840.
[4]周肇基.七彩缤纷彩叶芋[J].花木盆景(花卉园艺),2009(3):16-17.ZHOU Z J.Colorful Caladium bicolor[J].Flower Plant&Penjing,2009(3):16-17.
[5]周肇基.观叶新宠彩叶芋[J].花木盆景(花卉园艺),2000(12):4.ZHOU Z J.New favorite of foliage Caladium bicolor[J].Flower Plant&Penjing,2000(12):4
[6]周默.彩叶植物新品[J].中国花卉园艺,2016(6):56.ZHOU M.Colorful leaf plant[J].China Flowers&Horticulture,2016(6):56.
[7]蔡明.花叶芋组培快繁技术[J].内蒙古林业,1999(9):28.CAI M.Tissue culture and rapid propagation of Caladium bicolor[J].Inner Mongolia Forestry,1999(9):28.
[8]李国义,龚束芳,张丽梅,朱祥春,车代弟.彩色马蹄莲组培快繁技术的研究[J].北方园艺,2004(2):64-65.LI G Y,GONG S F,ZHANG L M,ZHU X C,CHE D D.Study on tissue culture and rapid propagation of Zantedeschia hybida[J].Northern Horticulture,2004(2):64-65.
[9]张东旭,周增产,卜云龙,张洁,张晓文,张成波,蔡伟健,康静,肖政.植物组织培养技术应用研究进展[J].北方园艺,2011(6):209-213.ZHANG D X,ZHOU Z C,BU Y L,ZHANG J,ZHANG X W,ZHANGC B,CAI W J,KANG J,XIAO Z.Research progress on plant tissue culture technology[J].Northern Horticulture,2011(6):209-213.
[10]PENCE,VALERIE C.Evaluating costs for the in vitro propagation and preservation of endangered plants[J].In Vitro Cellular&Developmental Biology,2011,47(1):176-187.
[11]黄祖传.绚丽多彩的彩叶芋[J].中国花卉盆景,1990(11):11.HUANG Z C.Bright and colourful Caladium bicolor[J].China Flower&Penjing,1990(11):11.
[12]董少鸣.彩叶芋的组织培养及快速繁殖[J].承德民族师专学报,2003,23(2):76.doi:10.16729/j.cnki.jhnun.2003.02.039.DONG S M.The histocultivating of colored-leaves taros and its rapid reproduction[J].Journal of Chengde Teachers'College for Nationalities,2003,23(2):76.doi:10.16729/j.cnki.jhnun.2003.02.039.
[13]王颖,陆国权.彩叶芋组织培养研究进展[J].北方园艺,2012(12):196-198.WANG Y,LU G Q.Research advances of Caladium bicolor[J].Northern Horticulture,2012(12):196-198.
[14]吴蓓,戴修纯,郑岩松,张木清,李光光,陈胜文,刘绍钦.大田苦瓜的离体快繁研究[J].广东农业科学,2017,44(12):27-32.doi:10.16768/j.issn.1004-874X.2017.12.005.WU B,DAI X C,ZHENG Y S,ZHANG M Q,LI G G,CHEN S W,LIUS Q.In vitro propagation of bitter gourd(Momordica charantia Linn.)for field culti-vation[J].Guangdong Agricultural Sciences,2017,44(12):27-32.doi:10.16768/j.issn.1004-874X.2017.12.005.
[15]高燕会,童再康,黄华宏,郭福起,郁重彦.花叶络石的组织培养[J].浙江农林大学学报,2006,23(6):701-704.GAO Y H,TONG Z K,HUANG H H,GUO F Q,YU C Y.Tissue culture on Trachelospermum jasminoides`Variegatum'[J].Journal of Zhejiang A&F University,2006,23(6):701-704.
[16]陈月桂,谭嘉娜,官锦燕,罗剑飘,黄海英,罗青文,杨俊贤,李奇伟.甘蔗新品种粤糖03-373组培快繁试验[J].广东农业科学,2017,44(5):13-18.doi:10.16768/j.issn.1004-874X.2017.05.003.CHEN Y G,TAN J N,GUAN J Y,LUO J P,HUANG H Y,LUOQ W,YANG J X,LI Q W.Tissue culture and rapid propagation of Yuetang 03-373,a new sugarcane variety[J].Guangdong Agricultural Sciences,2017,44(5):13-18.doi:10.16768/j.issn.1004-874X.2017.05.003.
[17]吴满良,李冬梅,朱根发,刘小飞.紫花山奈的组培快繁研究[J].广东农业科学,2018,45(7):29-33.doi:10.16768/j.issn.1004-874X.2018.07.005.WU M L,LI D M,ZHU G F,LIU X F.In vitro rapid propagation of Kaempferia elegans[J].Guangdong Agricultural Sciences,2018,45(7):29-33.doi:10.16768/j.issn.1004-874X.2018.07.005.
[18]蔡小东,DENG Z N.彩叶芋愈伤组织的诱导、增殖及植株再生[J].江苏农业科学,2016,44(9):75-77.doi:10.15889/j.issn.1002-1302.2016.09.021.CAI X D,DENG Z N.Callus induction,proliferation and plantlet regeneration of Caladium bicolor[J].Jiangsu Agricultural Sciences,2016,44(9):75-77.doi:10.15889/j.issn.1002-1302.2016.09.021.
[19]李维强,刘静.花叶芋组培快繁的应用研究[J].郑州牧业工程高等专科学校学报,2004(4):260-261.LI W Q,LIU J.Study on the technology of Caladium bicolor tissue culture and rapid multiplication[J].Journal of Zhengzhou College of Animal Husbandry&Engineering,2004(4):260-261.
[20]祝骥,姚汝华,黄毓文,梁承邺.苦丁茶愈伤组织的诱导与褐变抑制[J].热带亚热带植物学报,2000,8(4):319-323.ZHU J,YAO R H,HUANG Y W,LIANG C Y.Callus induction and browning inhibition in Ilex kudingcha C.J.TSENG[J].Journal of Tropical and Subtropical Botany,2000,8(4):319-323.
[21]罗阳春,杨云,李仕伟,方芳,王鹏蒲,全波,郑祖平.组织培养中植物外植体及愈伤组织褐变的研究进展[J].贵州农业科学,2018,46(1):5-10.LUO Y C,YANG Y,LI S W,FANG F,WANG P P,QUAN B,ZHENG ZP.Research progress on browning of explant and callus in plant tissue culture[J].Guizhou Agricultural Sciences,2018,46(1):5-10.
[22]BROOME O C,ZIMMERMAN R H.In vitro propagation of blackberry[J].Hortieulture Science,1978,13:151-153.
[23]肖小君,罗潼,王芳.木本植物组织培养过程中褐变现象及控制措施的研究进展[J].江苏农业科学,2017,45(16):20-24.doi:10.15889/j.issn.1002-1302.2017.16.004.XIAO X J,LUO T,WANG F.Research process on browning and control measures of woody plants tissue culture[J].Jiangsu Agricultural Sciences,2017,45(16):20-24.doi:10.19415/j.cnki.1673-890x.2015.36.009.
[24]任辉丽.植物组培技术常见问题及其预防措施[J].南方农业,2015,36(9):20-21.doi:10.19415/j.cnki.1673-890x.2015.36.009.REN H L.Common problems and preventive measures of plant tissue culture technology[J].South China Agriculture,2015,36(9):20-21.doi:10.19415/j.cnki.1673-890x.2015.36.009.
[25]姚丽娟,徐晓薇,林绍生,陈中林,陈义增.洋兰组培快繁褐变抑制因子探讨[J].北方园艺,2006(4):162-163.YAO L J,XU X W,LING S S,CHEN Z L,CHEN Y Z.Inhibitor of browning in tissue culture of orchid[J].Northern Horticulture,2006(4):162-163.
[26]贾荟芹,高龙梅,李薇,杨小丽,胡振兴.黄花菜组织培养褐变研究[J].农业开发与装备,2015(8):69.JIA H Q,GAO L M,LI W,YANG X L,HU Z X.Study on browning in tissue culture of Hemerocallis citrine Baroni[J].Agricultural Development&Equipments,2015(8):69.
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