水稻与弱毒丝核菌互作中免疫相关基因表达研究
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摘要
水稻纹枯病的病原菌为立枯丝核菌(Rhizoctonia solani Kǜhn)。用10株弱毒丝核菌提前接种水稻后,再接种纹枯病病原菌,观察其发病症状,并用实时荧光定量RT-PCR方法研究了接种不同处理后的水稻9个抗性基因表达情况,筛选出参与水稻纹枯病抗病过程的基因和能使水稻提前获得系统抗性的弱毒丝核菌菌株。结果显示,参与水稻诱导抗性的抗性基因主要为PR10a、ORK10、NAC4、WRKY53、WRKY71。大部分弱毒双核丝核菌能诱导水稻抗性基因表达,诱导效果最好的弱毒丝核菌菌株是②BS-J-06-8-1、④chd-YT-3-5和⑨DL-YT-06-4-9。
The pathogen of rice sheath blight is Rhizoctonia solani Kǜhn. In this study, 10 strains of attenuated R. solani were inoculated with rice in advance. And the pathogen was inoculated and the symptoms were observed. The expression of 9 resistance genes in rice treated with different treatments was studied with RT-PCR, and screening was carried out for the genes involved in resistance process of rice sheath blight and the strains of R. solani which can obtain the system resistance in advance. The results showed that the main-involved genes were PR10 a, ORK10, NAC4, WRKY53 and WRKY71. Most of the attenuated R. solani can induce the expression of rice resistance genes, and the strains with the best induction effect were: ②BS-J-06-8-1, ④chd-YT-3-5 and ⑨DL-YT-06-4-9.
The pathogen of rice sheath blight is Rhizoctonia solani Kǜhn. In this study, 10 strains of attenuated R. solani were inoculated with rice in advance. And the pathogen was inoculated and the symptoms were observed. The expression of 9 resistance genes in rice treated with different treatments was studied with RT-PCR, and screening was carried out for the genes involved in resistance process of rice sheath blight and the strains of R. solani which can obtain the system resistance in advance. The results showed that the main-involved genes were PR10 a, ORK10, NAC4, WRKY53 and WRKY71. Most of the attenuated R. solani can induce the expression of rice resistance genes, and the strains with the best induction effect were: ②BS-J-06-8-1, ④chd-YT-3-5 and ⑨DL-YT-06-4-9.
引文
[1]任春梅，高必达，何迎春.水稻抗纹枯病的研究进展[J].植物保护，2001,27(4):32-36.
[2]Yang G H,Chen H R,Naito S,et al.First Report of AG-A of binucleate Rhizoctonia in China,pathogenic to soya bean,Pea,Snap Bean and Pak Choy[J].Journal of Phytopathology,2010,153(6):333-336.
[3]Roche S A,Carter R J,Peakall R,et al.A narrow group of monophyletic Tulasnella(Tulasnellaceae)symbiont lineages are associated with multiple species of Chiloglottis(Orchidaceae):Implications for orchid diversity[J].American Journal of Botany,2010,97(8):1313-1327.
[4]段春芳，李枝林，方飞，等.云南几种兰花菌根真菌的分离鉴定[J].西南农业学报，2010,23(3):756-759.
[5]Carling D,Baird E,Gitaitis K A,et al.Characterization of AG-13,a newly reported anastomosis group of Rhizoctonia solani[J].Phytopathology,2002,92(8):893-899.
[6]Sharon M,Kuninaga S,Hyakumachi M,et al.The advancing identification and classification of Rhizoctonia spp.using molecular and biotechnological methods compared with the classical anastomosis grouping[J].Mycoscience,2006,47(6):299-316.
[7]Yang G H,Conner R L,Chen Y Y,et al.Frequency and pathogenicity distribution of Rhizoctonia spp.causing sheath blight on rice and banded leaf disease on maize in Yunnan,China.[J].Journal of Plant Pathology,2008,90(2):387-392.
[8]Yang G H,Chen J Y,Pu W Q.First report of head rot of cabbage and web-blight of snap bean caused by Rhizoctonia solani AG 4 HG-I[J].Plant Pathology,2010,56(2):351-351.
[9]Yang G H,Chen H R,Naito S,et al.Occurrence of foliar rot of pak choy and Chinese mustard caused by Rhizoctonia solani AG1-IB in China[J].Journal of General Plant Pathology,2005,71(5):377-379.
[10]Yang G H,Chen X Q.First report of foliar blight in Brassica rapa ssp.chinensis caused by Rhizoctonia solani AG4[J].Plant Pathology,2010,53(2):260-260.
[11]Brogue K,Chet I,Holliday M,et al.Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani[J].Science,1991,254(5035):1194-1197.
[12]Lin W,Anuratha C,Datta K,et al.Genetic engineering of rice for resistance to sheath blight[J].Nature Biotechnology,1995,13(7):686-691.
[13]Delaney T P,Uknes S,Vernooij B,et al.A central role of salicylic acid in plant disease resistance[J].Science,1994,266(5188):1247-1250.
[14]Midoh N,Iwata M.Cloning and characterization of a probenazole-inducible gene for an intracellular pathogenesis-related protein in rice[J].Plant and Cell Physiology,1996,37(1):9-18.
[15]Schweizer P,Buchala A,Dudler R,et al.Induced systemic resistance in wounded rice plants[J].The Plant Journal,1998,14(4):475-481.
[16]Suharsono U,Fujisawa Y,Kawasaki T,et al.The heterotrimeric G proteinαsubunit acts upstream of the small GTPase Rac in disease resistance of rice[J].Proceedings of the National Academy of Sciences,2002,99(20):13307-13312.
[17]Sawada K,Hasegawa M,Tokuda L,et al.Enhanced resistance to blast fungus and bacterial blight in transgenic rice constitutively expressing Os SBP,a rice homologue of mammalian selenium-binding proteins[J].Bioscience,Biotechnology and Biochemistry,2004,68(4):873-880.
[2]Yang G H,Chen H R,Naito S,et al.First Report of AG-A of binucleate Rhizoctonia in China,pathogenic to soya bean,Pea,Snap Bean and Pak Choy[J].Journal of Phytopathology,2010,153(6):333-336.
[3]Roche S A,Carter R J,Peakall R,et al.A narrow group of monophyletic Tulasnella(Tulasnellaceae)symbiont lineages are associated with multiple species of Chiloglottis(Orchidaceae):Implications for orchid diversity[J].American Journal of Botany,2010,97(8):1313-1327.
[4]段春芳，李枝林，方飞，等.云南几种兰花菌根真菌的分离鉴定[J].西南农业学报，2010,23(3):756-759.
[5]Carling D,Baird E,Gitaitis K A,et al.Characterization of AG-13,a newly reported anastomosis group of Rhizoctonia solani[J].Phytopathology,2002,92(8):893-899.
[6]Sharon M,Kuninaga S,Hyakumachi M,et al.The advancing identification and classification of Rhizoctonia spp.using molecular and biotechnological methods compared with the classical anastomosis grouping[J].Mycoscience,2006,47(6):299-316.
[7]Yang G H,Conner R L,Chen Y Y,et al.Frequency and pathogenicity distribution of Rhizoctonia spp.causing sheath blight on rice and banded leaf disease on maize in Yunnan,China.[J].Journal of Plant Pathology,2008,90(2):387-392.
[8]Yang G H,Chen J Y,Pu W Q.First report of head rot of cabbage and web-blight of snap bean caused by Rhizoctonia solani AG 4 HG-I[J].Plant Pathology,2010,56(2):351-351.
[9]Yang G H,Chen H R,Naito S,et al.Occurrence of foliar rot of pak choy and Chinese mustard caused by Rhizoctonia solani AG1-IB in China[J].Journal of General Plant Pathology,2005,71(5):377-379.
[10]Yang G H,Chen X Q.First report of foliar blight in Brassica rapa ssp.chinensis caused by Rhizoctonia solani AG4[J].Plant Pathology,2010,53(2):260-260.
[11]Brogue K,Chet I,Holliday M,et al.Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani[J].Science,1991,254(5035):1194-1197.
[12]Lin W,Anuratha C,Datta K,et al.Genetic engineering of rice for resistance to sheath blight[J].Nature Biotechnology,1995,13(7):686-691.
[13]Delaney T P,Uknes S,Vernooij B,et al.A central role of salicylic acid in plant disease resistance[J].Science,1994,266(5188):1247-1250.
[14]Midoh N,Iwata M.Cloning and characterization of a probenazole-inducible gene for an intracellular pathogenesis-related protein in rice[J].Plant and Cell Physiology,1996,37(1):9-18.
[15]Schweizer P,Buchala A,Dudler R,et al.Induced systemic resistance in wounded rice plants[J].The Plant Journal,1998,14(4):475-481.
[16]Suharsono U,Fujisawa Y,Kawasaki T,et al.The heterotrimeric G proteinαsubunit acts upstream of the small GTPase Rac in disease resistance of rice[J].Proceedings of the National Academy of Sciences,2002,99(20):13307-13312.
[17]Sawada K,Hasegawa M,Tokuda L,et al.Enhanced resistance to blast fungus and bacterial blight in transgenic rice constitutively expressing Os SBP,a rice homologue of mammalian selenium-binding proteins[J].Bioscience,Biotechnology and Biochemistry,2004,68(4):873-880.