摘要
<正>表观遗传机制可调控植物对逆境胁迫的应答,组蛋白变体H2A.Z为重要的表观修饰因子,参与多种非生物胁迫~([1-3]),并且,其基因家族成员较多~([4-5])。hta9hta11双突变体植株具有多效性表型,且H2A.Z基因家族其他成员的表达不能补偿HTA9和HTA11蛋白功能缺失~([6])。番茄(Lycopersicon esculentum Mill.)为重要的设施蔬菜和常用的模式植物,对低温敏感~([7])。亚低温条件下番茄hta9hta11双突变体
Taking day temperature of 25 ℃ and night temperature of 20 ℃ as the control group, variations of photosynthetic carbon assimilation related indexes of wild type and hta9 hta11 double mutant of Lycopersicon esculentum Mill. in treatment group(day temperature of 15 ℃ and night temperature of 10 ℃) were compared. The results show that chlorophyll content in hta9 hta11 double mutant in treatment group is slightly lower than that of wild type; its net photosynthetic rate, contents of sucrose and starch, activities of fructose-1,6-bisphosphate aldolase and fructose-1,6-bisphosphatase, and relative expression levels of TK, GAPDH, SBPase, PRK, RbcL, RCA, FBPase and FBA genes are significantly lower than those of wild type; while its ribulose-1,5-bisphosphate carboxylase/oxygenase activity and relative expression level of RbcS gene are significantly higher than those of wild type. It is suggested that photosynthetic carbon assimilation ability of hta9 hta11 double mutant of L. esculentum is lower than that of wild type under mild hypothermia condition, and histone variant H2 A.Z may play an important role in regulating photosynthetic carbon assimilation pathway of L. esculentum.
引文
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