蒙药蓝盆花对大鼠急性肝损伤的保护作用研究
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摘要
目的:研究蒙药兰盆花对大鼠急性肝损伤的保护作用.方法:将实验大鼠随机分为正常对照组、急性肝损伤模型组、阳性药物组和蓝盆花低、中、高剂量组.空白组与模型组动物均灌胃蒸馏水(1次/d,14d),阳性药物组和蓝盆花低、中、高剂量组均按实验剂量连续灌胃给药(1次/d,14d).末次给药后腹腔注射D-氨基半乳糖/脂多糖(300mg·kg~(-1)/0.1mg·kg~(-1)),6h后取血,测定实验大鼠血清中与肝脏功能损伤相关指标及炎性因子的变化情况.结果:模型组大鼠血清中肝脏功能指标ALT、AST和TBA水平升高;炎症因子指标肿瘤坏死因子α(TNF-α)、白介素-6(IL-6)水平升高,白介素-10(IL-10)水平降低.给予蓝盆花可显著改善肝功能,表现出一定的抗炎作用,可以降低ALT、AST、TBA、TNF-α和IL-6的水平,升高IL-10的水平,缓解由D-氨基半乳糖联合脂多糖引起的急性肝损伤.结论:蓝盆花对D-氨基半乳糖联合脂多糖所致大鼠急性肝功能损伤有保护作用.
Objective:To investigate the protective effect of Mongolian medicine Scabiosa tschilliensis on acute liver injury induced by D-galactosamine/lipopolysaccharide in rats.Methods:The expenimental rats were randomly divided into normal control group,acute liver injury model group,positive drug group and Scabiosa tschilliensis of low,medium and high dose group. The rats in normal control group and model group were given distilled water once a day,while those in other drug treatment groups were orally given drugs according to the experimental dose once a day and for 14 days. D-galactosamine/lipopolysaccharide(300 mg·kg~(-1)/0.1 mg·kg~(-1))was injected intraperitoneally after the last administration. Serum was collected six hours later to observe the changes of liver injury and related inflammatory factors.Results:The serum levels of ALT,AST and TBA,TNF-α,IL-6,and IL-10 in the model group were increased,while the levels of IL-10 were decreased. Scabiosa tschilliensis can significantly improve liver function,show certain anti-inflammatory effect,reduce the levels of ALT,AST,TBA,TNF-α and IL-6,increase the level of IL-10,alleviate liver injury caused by D-galactosamine combined with lipopolysaccharide.Conclusion:Scabiosa tschilliensis can significantly improve acute liver injury induced by D-galactosamine combined with lipopolysaccharide in rats.
Objective:To investigate the protective effect of Mongolian medicine Scabiosa tschilliensis on acute liver injury induced by D-galactosamine/lipopolysaccharide in rats.Methods:The expenimental rats were randomly divided into normal control group,acute liver injury model group,positive drug group and Scabiosa tschilliensis of low,medium and high dose group. The rats in normal control group and model group were given distilled water once a day,while those in other drug treatment groups were orally given drugs according to the experimental dose once a day and for 14 days. D-galactosamine/lipopolysaccharide(300 mg·kg~(-1)/0.1 mg·kg~(-1))was injected intraperitoneally after the last administration. Serum was collected six hours later to observe the changes of liver injury and related inflammatory factors.Results:The serum levels of ALT,AST and TBA,TNF-α,IL-6,and IL-10 in the model group were increased,while the levels of IL-10 were decreased. Scabiosa tschilliensis can significantly improve liver function,show certain anti-inflammatory effect,reduce the levels of ALT,AST,TBA,TNF-α and IL-6,increase the level of IL-10,alleviate liver injury caused by D-galactosamine combined with lipopolysaccharide.Conclusion:Scabiosa tschilliensis can significantly improve acute liver injury induced by D-galactosamine combined with lipopolysaccharide in rats.
引文
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[12]王小众,张莉娟. IL-10抗肝纤维化的实验研究及临床现状[J].世界华人消化杂志,2007,15(23):2469-2472.
[2]朱亚明.内蒙古植物药志[M].呼和浩特:内蒙古人民出版社,2000:113.
[3]中华人民共和国卫生部药典委员会.中华人民共和国卫生部药品标准蒙药分册[S]. 1998:52.
[4]国家中医药管理局中华本草编委会.中华本草·蒙药卷[M].上海:上海科学技术出版社,2004:385.
[5]罗布桑.蒙药学[M].呼和浩特:内蒙古人民出版社,2006:251.
[6]王国英.蒙药蓝盆花化学成分及质量控制研究[D].呼和浩特:内蒙古医学院,2010.
[7]王福根,孙静霞.肝损伤动物模型的建立和应用[J].中国药房,2006,17(6):702.
[8]李雪峰.血清TBA、ADA、CHE及PA检测诊断肝病的价值[J].放射免疫学杂志,2012,25(2):214-215.
[9]易敏,付元元.血清TBA比值的测定在肝病诊断中的临床意义[J].实用预防医学,2011,18(7):1337-1339.
[10]黄振青,韦燕飞,刘雪梅,等.穗花杉双黄酮对急性肝损伤大鼠炎症相关因子的影响[J].中国实验方剂学杂志,2014,20(11):147-150.
[11]庄敏之,王亚平,郑昕,等.护肝解毒方对药物性肝损伤及相关炎症因子作用研究[J].中国中西医结合消化杂志,2016,24(1):26-28.
[12]王小众,张莉娟. IL-10抗肝纤维化的实验研究及临床现状[J].世界华人消化杂志,2007,15(23):2469-2472.
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