阿胶肽制备过程中脱脂工艺研究
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摘要
以阿胶为原料,经蛋白酶酶解得酶解液,通过脱脂剂选择,结果活性炭脱脂效果好于海藻酸钠,以油脂含量为指标,经单因素响应面优化活性炭脱脂工艺,结果为pH 7.63,温度26.04℃,固液比1∶38.3(W/V)时,此时的脱脂率为98.84%。
As raw material,Colla corii asini was enzymatically hydrolyzed by protease to obtain enzymatic hydrolysate.Different degreasing agents were selected to prove that the degreasing effect of activated carbon is better than that of sodium alginate.With the oil content as the index,the activated carbon degreasing process which was optimized by single-factor response surface gave a degreasing rate of 98.84% under the following treatment conditions:pH 7.63,26.04 ℃,solid-liquid ratio 1∶38.3(W/V).
As raw material,Colla corii asini was enzymatically hydrolyzed by protease to obtain enzymatic hydrolysate.Different degreasing agents were selected to prove that the degreasing effect of activated carbon is better than that of sodium alginate.With the oil content as the index,the activated carbon degreasing process which was optimized by single-factor response surface gave a degreasing rate of 98.84% under the following treatment conditions:pH 7.63,26.04 ℃,solid-liquid ratio 1∶38.3(W/V).
引文
[1] 高景会,王蕊,范锋.阿胶现代研究进展 [J].中国药事,2011(4):396-401.
[2] 毛跟年,郭倩,瞿建波,等.阿胶化学成分及药理作用研究进展 [J].动物医学进展,2010(11):83-85.
[3] 郑筱祥,杨勇,叶剑锋,等.东阿阿胶的升白作用及机制研究 [J].中国现代应用药学,2005(2):102-105.
[4] 田昌林,鲍忠剑,田文儒.丙酮法和碱法脱脂对胶原蛋白生产的影响[J].动物医学进展,2007,28(4):44-46.
[5] 陈丽清,陈清,韩佳冬,等.猪皮超声波乳化脱脂工艺的研究[J].食品工业科技,2012,16(33):265-267.
[6] 赵帅,巩旭,李国英.猪皮的不同脱脂方法对胶原提取率的影响[J].中国皮革,2007,36(9):33-36.
[7] 李建刚,李开喜,凌立成,等.成型活性炭的制备及其甲烷吸附性能的研究[J].新型炭材料,2004,19(2) :114-118.
[8] 刘常洪,杨思敬.关于煤甲烷吸附体系吸附规律的试验研究[J].煤矿安全,1992(4):1-5.
[9] 刘海燕,凌立成,刘植昌,等.甲烷在高比表面积活性炭上吸附行为的初步研究[J].天然气化工:C1化学与化工,1999,24(1):6-9.
[10] 曹晓强,黄学敏,刘胜荣,等.2种改性活性炭对甲苯吸附性能的对比研究[J].环境科学,2008,29(10):2868-2873.
[11] SHEN W Z,LI Z J,LIU Y H.Surface chemical functional groupsmodification of porous carbon[J].Recent Patents on Chemical Engineering,2008,1(1) :27-40.
[12] SHIM J W,PARK S J,RYU S K.Effect of modification with HNO3and NaOH on metal adsorption by pitch-based activated carbon fibers[J].Carbon,2001,39(11):1635-1642.
[13] LIU S X,CHEN X,CHEN X Y,et al.Activated carbon with excellent chromium(VI) adsorption performance prepared by acid-base surface modification[J].Journal of Hazardous Materials,2007,141(1):315-319.
[14] JUNG M J,JEONG E,LEE S I,et al.Improved capacitance characteristics of activated carbon-based electrodes by physicochemical base-tuning[J].Journal of Industrial and Engineering Chemistry,2012,18(2):642-647.
[15] SUN C J,WANG Y,CUI H X,et al.Effect of surface modification on mesoporous activated carbon's adsorption capacity to two agro-antibiotics[J].Chinese Journal of Pesticide Science,2012,14(1):89-94.
[16] WANG G P,WU T,LI Y J,et al.Removal of ampicillin sodium in solution using activated carbon adsorption integrated with H2O2 oxidation[J].Journal of Chemical Technology and Biotechnology,2012,87(5):623-628.
[17] KIM K J,KANG C S,YOU Y J,et al.Adsorption-desorption characteristics of VOCs over impregnated activated carbons[J].Catalysis Today,2006,111(3-4):223-228.
[18] 刘守新,陈孝云,陈曦,等.表面酸碱2步改性对活性炭吸附Cr(Ⅵ) 的影响[J].环境科学,2005,26(6):89-93.
[19] 王孝平,邢树礼.考马斯亮蓝法测定蛋白含量的研究[J].天津化工,2009,23(3):40-42.
[20] 吴有炜.实验设计与数据处理[M].苏州:苏州大学出版社,2002.
[2] 毛跟年,郭倩,瞿建波,等.阿胶化学成分及药理作用研究进展 [J].动物医学进展,2010(11):83-85.
[3] 郑筱祥,杨勇,叶剑锋,等.东阿阿胶的升白作用及机制研究 [J].中国现代应用药学,2005(2):102-105.
[4] 田昌林,鲍忠剑,田文儒.丙酮法和碱法脱脂对胶原蛋白生产的影响[J].动物医学进展,2007,28(4):44-46.
[5] 陈丽清,陈清,韩佳冬,等.猪皮超声波乳化脱脂工艺的研究[J].食品工业科技,2012,16(33):265-267.
[6] 赵帅,巩旭,李国英.猪皮的不同脱脂方法对胶原提取率的影响[J].中国皮革,2007,36(9):33-36.
[7] 李建刚,李开喜,凌立成,等.成型活性炭的制备及其甲烷吸附性能的研究[J].新型炭材料,2004,19(2) :114-118.
[8] 刘常洪,杨思敬.关于煤甲烷吸附体系吸附规律的试验研究[J].煤矿安全,1992(4):1-5.
[9] 刘海燕,凌立成,刘植昌,等.甲烷在高比表面积活性炭上吸附行为的初步研究[J].天然气化工:C1化学与化工,1999,24(1):6-9.
[10] 曹晓强,黄学敏,刘胜荣,等.2种改性活性炭对甲苯吸附性能的对比研究[J].环境科学,2008,29(10):2868-2873.
[11] SHEN W Z,LI Z J,LIU Y H.Surface chemical functional groupsmodification of porous carbon[J].Recent Patents on Chemical Engineering,2008,1(1) :27-40.
[12] SHIM J W,PARK S J,RYU S K.Effect of modification with HNO3and NaOH on metal adsorption by pitch-based activated carbon fibers[J].Carbon,2001,39(11):1635-1642.
[13] LIU S X,CHEN X,CHEN X Y,et al.Activated carbon with excellent chromium(VI) adsorption performance prepared by acid-base surface modification[J].Journal of Hazardous Materials,2007,141(1):315-319.
[14] JUNG M J,JEONG E,LEE S I,et al.Improved capacitance characteristics of activated carbon-based electrodes by physicochemical base-tuning[J].Journal of Industrial and Engineering Chemistry,2012,18(2):642-647.
[15] SUN C J,WANG Y,CUI H X,et al.Effect of surface modification on mesoporous activated carbon's adsorption capacity to two agro-antibiotics[J].Chinese Journal of Pesticide Science,2012,14(1):89-94.
[16] WANG G P,WU T,LI Y J,et al.Removal of ampicillin sodium in solution using activated carbon adsorption integrated with H2O2 oxidation[J].Journal of Chemical Technology and Biotechnology,2012,87(5):623-628.
[17] KIM K J,KANG C S,YOU Y J,et al.Adsorption-desorption characteristics of VOCs over impregnated activated carbons[J].Catalysis Today,2006,111(3-4):223-228.
[18] 刘守新,陈孝云,陈曦,等.表面酸碱2步改性对活性炭吸附Cr(Ⅵ) 的影响[J].环境科学,2005,26(6):89-93.
[19] 王孝平,邢树礼.考马斯亮蓝法测定蛋白含量的研究[J].天津化工,2009,23(3):40-42.
[20] 吴有炜.实验设计与数据处理[M].苏州:苏州大学出版社,2002.