甜菜碱和水杨酸对干旱胁迫下辣椒开花结果期生理特性的影响
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摘要
以云椒2号为材料,研究外源甜菜碱(glycine betaine,GB,100 mmol/L)和水杨酸(salicylic acid,SA,200 mg/L)对干旱胁迫下辣椒开花结果期生理特性的影响。结果表明:在干旱胁迫下,外源GB,SA处理能有效缓解辣椒叶片相对含水量的下降,抑制丙二醛含量的增加,同时,提高辣椒叶片可溶性糖、脯氨酸的含量,提高过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)的活性;外源SA处理使辣椒叶片可溶性蛋白含量下降。利用模糊隶属函数法对渗透调节物质、膜脂过氧化和抗氧化酶活性多项指标进行综合评价,GB,SA、干旱处理综合评价值分别为0.521 9,0.495 0,0.475 1。GB,SA处理均能提高辣椒的抗旱性,其中GB处理的辣椒抗旱性更好。
The purpose of this study is to explore the effects of exogenous glycine betaine(GB, 100 mmol/L) and salicylic acid(SA, 200 mg/L) on physiological characters of Yunjiao 2 of hot pepper(Capsicum annuum L.) under drought stress during blossom and fruit period. The results show that, under the condition of drought stress, exogenous GB and SA could effectively relieve the reduction of relative water content in leaves, suppress the increase of malondialdehyde(MDA) content, increase the contents of soluble sugar and proline, and stimulate the activities of peroxidase(POD), superoxide dismutase(SOD) and catalase(CAT). Exogenous SA could diminish the content of soluble protein. The comprehensive assessment of multiple indicators including osmotic regulation substance, membrane lipid peroxidation and antioxidant enzyme activities was conducted by fuzzy subordination method, and the comprehensive evaluation values were 0.521 9, 0.495 0 and 0.475 1 under GB, SA and drought stress, respectively. In conclusion, exogenous GB and SA could improve drought resistance of hot pepper, in particular, the treatment of GB was more effective.
The purpose of this study is to explore the effects of exogenous glycine betaine(GB, 100 mmol/L) and salicylic acid(SA, 200 mg/L) on physiological characters of Yunjiao 2 of hot pepper(Capsicum annuum L.) under drought stress during blossom and fruit period. The results show that, under the condition of drought stress, exogenous GB and SA could effectively relieve the reduction of relative water content in leaves, suppress the increase of malondialdehyde(MDA) content, increase the contents of soluble sugar and proline, and stimulate the activities of peroxidase(POD), superoxide dismutase(SOD) and catalase(CAT). Exogenous SA could diminish the content of soluble protein. The comprehensive assessment of multiple indicators including osmotic regulation substance, membrane lipid peroxidation and antioxidant enzyme activities was conducted by fuzzy subordination method, and the comprehensive evaluation values were 0.521 9, 0.495 0 and 0.475 1 under GB, SA and drought stress, respectively. In conclusion, exogenous GB and SA could improve drought resistance of hot pepper, in particular, the treatment of GB was more effective.
引文
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[15]叶松涛,杜旭华,宋帅杰,等.水杨酸对干旱胁迫下毛竹实生苗生理生化特征的影响[J].林业科学,2015,51(11):25-31.
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[19]贺丽江,陈雪宇,李文略,等.干旱胁迫下喷施甜菜碱对苎麻生理特性及产量的影响[J].中国麻业科学,2015,37(3):130-134.
[20]SOFO A,DICHIO B,XILOYANNIS C,et al.Effects different irradiance levels on same antioxidant enzymes and on malondialdehyde content during rewatering in olive tree[J].Plant Science,2004,166:293-302.
[2]罗明华,罗英,王璞.水杨酸处理对干旱胁迫下丹参幼苗抗氧化能力的影响[J].干旱地区农业研究,2010,28(4):102-105.
[3]CHOUDHARY K K,AGRAWAL S B.Cultivar specificity of tropical mung bean(Vigna radiata L.)to elevated ultraviolet-B:Changes in antioxidative defense system,nitrogen metabolism and accumulation of jasmonic and salicylic acids[J].Environmental and Experimental Botany,2014,99:122-132.
[4]ARGUESO C T,FERREIRA F J,EPPLE P,et al.Twocomponent elements mediate interactions between cytokinin and salicylic acid in plant immunity[J].PLoS Genetics,2012,8(1):e1002448.
[5]可静,李进,李永洁.干旱胁迫下黑果枸杞幼苗对外源水杨酸的生理响应[J].植物生理学报,2016,52(4):497-504.
[6]张冬野,赵婷婷,李景富,等.水杨酸和氯化钙对干旱胁迫下番茄幼苗生理特性的影响[J].浙江农业学报,2016,28(10):1687-1694.
[7]王凤兰,黄子锋,梁淑贞,等.外源水杨酸对干旱胁迫下一品红生理特性的影响[J].西南师范大学学报(自然科学版),2016,41(2):53-57.
[8]李爱梅,张玲,张超,等.黄腐酸和甜菜碱预处理对干旱胁迫下平邑甜茶生理特性及光合的影响[J].西北植物学报,2017,37(2):307-314.
[9]张红敏,李姣姣,黑刚刚,等.外源甜菜碱处理对干旱胁迫下半夏氮代谢及相关酶活性的影响[J].草业学报,2014,23(4):229-236.
[10]邹琪.植物生理实验指导[M].北京:中国农业出版社,2000.
[11]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[12]王学奎.植物生理生化实验原理和技术[M].2版.北京:高等教育出版社,2000.
[13]陈文荣,曾玮玮,李云霞,等.高丛蓝莓对干旱胁迫的生理响应及其抗旱性综合评价[J].园艺学报,2012,39(4):637-646.
[14]徐建欣,杨洁,刘实忠,等.干旱胁迫对云南陆稻幼苗生理特性的影响[J].中国农学通报,2014,30(27):145-152.
[15]叶松涛,杜旭华,宋帅杰,等.水杨酸对干旱胁迫下毛竹实生苗生理生化特征的影响[J].林业科学,2015,51(11):25-31.
[16]CHAVES M M,OLIVEIRA M M.Mechanisms underlying plant resilience to water deficits:Prospects for watersaving agriculture[J].Journal of Experimental Botany,2005,55(407):2365-2384.
[17]安玉艳,梁宗锁,郝文芳.杠柳幼苗对不同强度干旱胁迫的生长与生理响应[J].生态学报,2011,31(3):716-725.
[18]常云霞,徐克东,杨同文,等.外源水杨酸对野生龙葵幼苗干旱胁迫的缓解效应[J].干旱地区农业研究,2014,32(4):43-46,64.
[19]贺丽江,陈雪宇,李文略,等.干旱胁迫下喷施甜菜碱对苎麻生理特性及产量的影响[J].中国麻业科学,2015,37(3):130-134.
[20]SOFO A,DICHIO B,XILOYANNIS C,et al.Effects different irradiance levels on same antioxidant enzymes and on malondialdehyde content during rewatering in olive tree[J].Plant Science,2004,166:293-302.
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