春砂仁和益智仁中黄酮类物质的精制及其抑菌和抗肿瘤功能研究
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摘要
目的提取分离春砂仁与益智仁中的黄酮类物质,并研究其抑菌、抗肿瘤功能。方法通过黄酮类物质提取率优化浸提黄酮类物质的乙醇浓度及大孔树脂分离条件,采用最小抑菌浓度及最小杀菌浓度法确定最有效抑菌组分,并研究春砂仁与益智仁的有效组分复配后对动物体内抗肿瘤的功效。结果在60%乙醇浸提条件下选用AB-8大孔树脂分离纯化春砂仁与益智仁中的黄酮类物质效果较好,经80%乙醇洗脱后的黄酮类物质具有较强的抑菌活性,且对小鼠移植性肿瘤S180、H22具有抑制作用。结论春砂仁和益智仁中黄酮类物质具有较强的抗菌、抗肿瘤活性。
Objective To extract and separate the flavonoids from Amomum villosum Lour and Alpinia oxyphylla Miq by macroporous resin, and study their antimicrobial and antineoplastic functions. Methods The optimum ethanol concentration and separation conditions of macroporous resin for extracting flavonoids were determined by tracking the extraction rate of flavonoids. The most effective antimicrobial components were determined by the minimum antimicrobial concentration and the minimum bactericidal concentration. The antitumor effect of the mixture of the effective components of Amomum villosum Lour and Alpinia oxyphylla Miq on the animal in vivo was studied. Results AB-8 macroporous resin had good effect, which was used to separate and purify the flavonoids in Amomum villosum Lour and Alpinia oxyphylla Miq under 60% ethanol extraction conditions. The flavonoids which were eluted by 80% ethanol had stronger bacteriostatic activity, which had inhibitory effect on S180 and H22 in mice. Conclusion The flavonoids in Amomum villosum Lour and Alpinia oxyphylla Miq have strong antibacterial and antitumor activities.
Objective To extract and separate the flavonoids from Amomum villosum Lour and Alpinia oxyphylla Miq by macroporous resin, and study their antimicrobial and antineoplastic functions. Methods The optimum ethanol concentration and separation conditions of macroporous resin for extracting flavonoids were determined by tracking the extraction rate of flavonoids. The most effective antimicrobial components were determined by the minimum antimicrobial concentration and the minimum bactericidal concentration. The antitumor effect of the mixture of the effective components of Amomum villosum Lour and Alpinia oxyphylla Miq on the animal in vivo was studied. Results AB-8 macroporous resin had good effect, which was used to separate and purify the flavonoids in Amomum villosum Lour and Alpinia oxyphylla Miq under 60% ethanol extraction conditions. The flavonoids which were eluted by 80% ethanol had stronger bacteriostatic activity, which had inhibitory effect on S180 and H22 in mice. Conclusion The flavonoids in Amomum villosum Lour and Alpinia oxyphylla Miq have strong antibacterial and antitumor activities.
引文
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[17]王秋红,曹琦,李斌,等.不同发酵时间对西瓜霜抗菌活性的影响及其有效部位筛选[J].中草药,2015,46(13):1950-1953.Wang QH,Cao Q,Li B,et al.Effect of fermentation time on antibacterial activity of Mirabilitum praeparatum and screening of its antibacterial active parts[J].Chin Tradit Herbal Drugs,2015,46(13):1950-1953.
[18]邓红梅,乐敏柔,王春.不同方法提取的柠檬精油挥发性成分比较及其抑菌性研究[J].中国食品添加剂,2018,(9):56-61.Deng HM,Le MR,Wang C.Comparison of different extraction methods on volatile components and antimicrobial activities of lemon essential oil[J].China Food Add,2018,(9):56-61.
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[20]常银子,仲山民,曹玉成,等.野菊花、金银花和芦荟复合提取液抑菌作用的研究[J].安徽农业科学,2007,35(16):4872-4874.Chang YZ,Zhong SM,Cao YC,et al.Study on antibacterial effects of compound extrations of Lonicera japonica Thunb,Flos chrysanthemum and Aloe[J].J Anhui Agric Sci,2007,35(16):4872-4874.
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[2]郑义,邵颖,陈安徽,等.益智仁总黄酮超声辅助提取工艺优化及其抗氧化活性[J].食品科学,2014,35(6):44-49.Zheng Y,Shao Y,Chen AH,et al.Optimization of ultrasonic-assisted extraction and antioxidant activities of total flavonoids from Alpinia oxyphylla fruits[J].Food Sci,2014,35(6):44-49.
[3]吴德玲,金传山,刘金旗,等.益智仁中总黄酮含量测定[J].安徽中医学院学报,2005,(6):38-40.Wu DL,Jin CS,Liu JQ,et al.Determination of total flavonoids in Alpinia oxyphylla[J].J Anhui Univ Chin Med,2005,(6):38-40.
[4]钟旭美,陈铭中,彭嘉铭.春砂仁总黄酮提取物的抗氧化性及抑菌性研究[J].食品安全质量检测学报,2018,9(13):3335-3339.Zhong XM,Chen MZ,Peng JM.Antioxidant and antibacterial activities of the crude extract of total flavonoids from Amomum villosum Lour[J].JFood Saf Qual,2018,9(13):3335-3339.
[5]付琛,陈程,周光雄,等.阳春砂仁化学成分研究[J].中草药,2011,42(12):2410-2412.Fu C,Chen C,Zhou GX,et al.Chemical constituents from fruits of Amomum villosum[J].Chin Tradit Herbal Drugs,2011,42(12):2410-2412.
[6]Morkawa T,Matsuda H,Toguchida I,et al.Absolute stereostructures of three new sesquiterpenes from the fruit of Alpinia oxyphylla with inhibitory effects on nitric oxide production and degranulation in RBL-2H3 cells[J].J Nat Prod,2002,65(10):1468-1474.
[7]Heis HA,Banihani KE,Banihani BK.Adrenal cavernous haemangioma[J].Singapore Med J,2008,49(9):236-237.
[8]He ZH,Ge W,Yue GG,et al.Anti-angiogenic effects of the fruit of Alpinia oxyphylla[J].J Ethnopharmacol,2010,132(2):443-449.
[9]黄梅桂,徐云巧,张忠明,等.薰衣草残渣中黄酮的超声辅助提取工艺及其抗氧化活性[J].食品工业科技,2018,39(1):214-220.Huang MG,Xu YQ,Zhang ZM,et al.Flavonoids extracted by ultrasonic assisted method from lavender residue and its antioxidant activity[J].Sci Technol Food Ind,2018,39(1):214-220.
[10]张宏梅,昌盛,崔佰吉,等.苦瓜总黄酮的提取及其体外抗氧化活性分析[J].食品研究与开发,2016,37(23):61-64.Zhang HM,Chang S,Cui BJ,et al.Extraction and antioxidant activity in vitro of flavonoids from balsam pear[J].Food Res Dev,2016,37(23):61-64.
[11]姚新鼎,姜飞燕,王玉伟,等.微波辅助提取山竹壳中的总黄酮及其体外抗氧化活性研究[J].食品研究与开发,2019,40(4):114-118.Yao XD,Jiang FY,Wang YW,et al.Microwave assisted extraction of the speculator nobles bark flavonoids and its antibacterial research[J].Food Res Dev,2019,40(4):114-118.
[12]杨秀东,张嫔妹,张扬,等.托盘根不同溶剂萃取物的活性成分及其体外抗氧化活性[J].食品工业科技,2018,39(21):77-80.Yang XD,Zhang BM,Zhang Y,et al.Active composition of different solvent extracts of rubus crategefolius bunge root and their antioxidant activity in vitro[J].Sci Technol Food Ind,2018,39(21):77-80.
[13]杨波,韩凤波,杨波.AB-8和D-101大孔吸附树脂分离纯化玉竹多糖[J].食品研究与开发,2014,35(23):67-70.Yang B,Han FB,Yang B.Isolation&purification of polysaccharide from polygonatum odoratum by AB-8 and D-101 macroporous adsorption resins[J].Food Res Dev,2014,35(23):67-70.
[14]董华群,张英慧,黄剑波,等.荔枝黄酮提取和大孔树脂分离及其组分的HPLC分析[J].中国实验方剂学杂志,2012,18(11):111-115.Dong HQ,Zhang YH,Huang JB,et al.Litchi flavonoid extraction and separation with macroporous resins and the constituents analysis with HPLC[J].Chin J Exp Tradit Med Form,2012,18(11):111-115.
[15]苗修港,余翔,张贝贝,等.NKA-9大孔树脂纯化香椿叶黄酮类物质工艺优化[J].食品科学,2016,37(8):32-38.Miao XG,Yu X,Zhang BB,et al.Purification of flavonoids from Toona sinensis leaves with NKA-9 macroporous resin[J].Food Sci,2016,37(8):32-38.
[16]钟美,江丹,黄亮.对改良的三种最小抑菌浓度测试方法的探讨[J].中国洗涤用品工业,2016,(2):75-79.Zhong M,Jiang D,Huang L.Discussion on the improved three minimum inhibitory concentration test methods[J].China Detergent Ind,2016,(2):75-79.
[17]王秋红,曹琦,李斌,等.不同发酵时间对西瓜霜抗菌活性的影响及其有效部位筛选[J].中草药,2015,46(13):1950-1953.Wang QH,Cao Q,Li B,et al.Effect of fermentation time on antibacterial activity of Mirabilitum praeparatum and screening of its antibacterial active parts[J].Chin Tradit Herbal Drugs,2015,46(13):1950-1953.
[18]邓红梅,乐敏柔,王春.不同方法提取的柠檬精油挥发性成分比较及其抑菌性研究[J].中国食品添加剂,2018,(9):56-61.Deng HM,Le MR,Wang C.Comparison of different extraction methods on volatile components and antimicrobial activities of lemon essential oil[J].China Food Add,2018,(9):56-61.
[19]陈乃东,周守标,王春景,等.春花胡枝子黄酮类化合物的提取及清除羟自由基作用的研究[J].食品科学,2007,(1):86-91.Chen ND,Zhou SB,Wang CJ,et al.Study on flavonoids extraction and function of scavenging hydroxy free radical from Lespeda dunnii[J].Food Sci,2007,(1):86-91.
[20]常银子,仲山民,曹玉成,等.野菊花、金银花和芦荟复合提取液抑菌作用的研究[J].安徽农业科学,2007,35(16):4872-4874.Chang YZ,Zhong SM,Cao YC,et al.Study on antibacterial effects of compound extrations of Lonicera japonica Thunb,Flos chrysanthemum and Aloe[J].J Anhui Agric Sci,2007,35(16):4872-4874.
[21]刘泽鑫.植物源天然食品防腐剂的研究进展及前景[J].吕梁学院学报,2013,3(2):40-42.Liu ZX.Research progress and prospects of plant-derived natural food preservatives[J].J Lvliang Univ,2013,3(2):40-42.
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