星点设计—响应面法优化超声提取南烛叶总黄酮工艺条件

详细信息    查看全文 | 推荐本文 |

英文篇名：Optimization of Ultrasonic Extraction of Total Flavonoids from Vaccinium Bracteatum Thunb Leaves by Central Composite Design Response Surface Methodology 作者：周小祝 ; 张璐 英文作者：ZHOU Xiaozhu;ZHANG Lu;Nanfang Hospital,Southern Medical University;College of Traditional Chinese Medicine,Southern Medical University; 关键词：南烛叶 ; 总黄酮 ; 超声提取法 ; 星点设计—响应面法 英文关键词：Vaccinium bracteatum Thunb leaves;;total flavonoids;;ultrasonic extraction;;central composite design response surface method 中文刊名：辽宁中医药大学学报 英文刊名：Journal of Liaoning University of Traditional Chinese Medicine 机构：南方医科大学南方医院;南方医科大学中医药学院; 出版日期：2019-01-16 16:34 出版单位：辽宁中医药大学学报 年：2019 期：02 基金：广东省中医局科技计划项目(20172095) 语种：中文; 页：53-57 页数：5 CN：21-1543/R ISSN：1673-842X 分类号：R284.2

摘要

目的:采用星点设计—响应面法进行试验设计,建立模型优化南烛叶总黄酮的提取工艺,获得最佳提取条件。方法:该实验以芦丁作为成分指标测定南烛叶提取液中总黄酮含量;以提取温度、液料比、乙醇浓度作为自变量,黄酮得率作为响应值,进行星点设计试验,通过design-expert软件建立模型,分析单因素和交互项对南烛叶提取液中总黄酮得率的影响。结果:经design-expert对各因素进行多元二次拟合所得方程为Y=-137.93+2.62A+1.32B+1.73C-4.72×10~(-3)AB-8.25×10~(-3)AC-5.10×10~(-3)BC-0.02A2-8.83×10~(-3)B~2-8.68×10~(-3)C~2,通过模型进行最佳提取工艺的优化,结合实际操作的调整,提取温度46℃、液料比45:1、乙醇浓度65%为最佳提取条件,平行试验3次的总黄酮得率与预测值的偏差为-1.15%。结论:星点设计—响应面方法使用简便,对实际生产过程中提取条件的优化可得到很好的预测结果。
        Objective:To optimize the extraction process of flavonoids from Vaccinium bracteatum Thunb leaves by using central composite design response surface method(CCD-RSM). The optimum extraction conditions were obtained. Methods:This experiment used rutin as a component index for the determination of the total flavonoidsin extract of Vaccinium bracteatum Thunb leaves. Extraction temperature,solid-to-liquid ratio and ethanol concentration were as independent variables and the yield of flavonoids were as the response value of central composite design. The test model was established by designexpert software,analyzing the influence of single factor and interaction on the extraction yield of flavonoids from Vaccinium bracteatum Thunb leaves. Results:Considering each factor in this experiment,a multiple quadratic equation was formulated by design-expert as follows:Y=-137.93+2.62A+1.32B+1.73C-4.72×10~(-3)AB-8.25×10~(-3)AC-5.10×10~(-3)BC-0.02A~2-8.83×10~(-3)B~2-8.68×10~(-3)C~2. To optimize the best extraction process through the model,combined with the adjustment in actual operation. Extraction temperature 46 ℃,solid-to-liquid ratio 45∶1,ethanol concentration of 65% were proved to be the best extraction conditions.During the 3 times parallel tests,the deviation of the obtainedrate of flavonoids and the predictive value was -1.5%. Conclusion:The central composite design response surface method(CCD-RSM)is simple and convenient,and it can be used to optimize the extraction conditions in actual production process.

引文

[1]李增亮,张琳,田景奎,等.乌饭树叶的化学成分研究[J].中国中药杂志,2008,33(18):2087-2089.
    [2]陈明,王举涛,吴珍妮,等.半枝莲总黄酮通过PI3K/AKT/mTOR通路诱导肿瘤细胞自噬的体内实验研究[J].中国中药杂志,2017,42(7):1358-1364.
    [3]Qinglin L,Xin W,Zhong L,et al. A study on the anti-tumor mechanism of total flavonoids from Radix Tetrastigmae against additional cell line based on COX-2-mediated Wnt/β-catenin signaling pathway[J]. Oncotarget,2017,8(33):54304-54319.
    [4]Xiang M,Su H,Hong Z,et al.Chemical composition of total flavonoids from Polygonum amplexicaule and their pro-apoptotic effect on hepatocellular carcinoma cells:Potential roles of suppressing STAT3 signaling[J]. Food Chem Toxicol,2015,80:62-71.
    [5]Dai ZJ,Wang BF,Lu WF,et al. Total flavonoids of Scutellaria barbata inhibit invasion of hepatocarcinoma via MMP/TIMP in vitro[J]. Molecules, 2013,18(1):934-950.
    [6]余清.乌饭树叶中黄酮等有效成分分析及抗肿瘤作用研究[D].福州:福建农林大学,2008.
    [7]张德谨,陈义勇.荧光分光光度法测定乌饭树树叶中总黄酮含量[J].食品工业,2014,35(6):273-277.
    [8]黄佳萍,郑青,郭梦梦,等.超声波法提取乌饭树叶槲皮素的工艺研究[J].安徽农业科学,2013,41(9):4050-4052.
    [9]余清,郑小严,黄红霞,等.超临界CO2萃取乌饭树叶总黄酮的工艺[J].福建农林大学学报(自然科学版),2009,38(1):97-102.
    [10]任凤莲,谷芳芳,吴梅林,等.利用响应面分析法优化山楂中总黄酮提取条件[J].天然产物研究与开发,2006,18:126-129.
    [11]王振忠,武文洁.野菊花总黄酮提取工艺的响应面设计优化[J].时珍国医国药,2007,18(3):648-650.
    [12]林勇,朱良辉,吴永忠.比色法测定不同产地乌饭叶总黄酮含量[J].时珍国医国药,1999,10(2):97-97.