新疆地区淡水贝类的形态学及分子分类研究
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摘要
为了解新疆地区淡水贝类种类及地理分布情况,采用形态学与分子生物学相结合的方法对新疆12个地区采集的淡水贝类进行分类鉴定。利用基于线粒体DNA (mt DNA)COI基因、核糖体内转录间隔序列2(ITS2)基因的DNA条形码技术进行PCR扩增、克隆及测序,对其进行分子分类鉴定。结果发现,21种淡水贝类主要隶属于腹足纲(Gastropoda)和双壳纲(Bivalvia),共4目、10科、14属;其中隶属双壳纲的贝类仅有1科1种;隶属于腹足纲的淡水螺占9科、13属,分属田螺科1种、豆螺科1种、扁卷螺科2种、椎实螺科7种、膀胱螺科2种、巴蜗牛科1种、坚齿螺科4种、琥珀螺科1种、嗜石螺科1种。
In order to investigate the geographical distribution and species of freshwater mollusks, morphological, anatomical and molecular biological techniques were used to identify and classify freshwater mollusks collected from 12 areas of Xinjiang. COI and ITS2 gene fragments were amplified respectively by PCR with specific primers, cloned and sequenced. The molecular identification and phylogenetic analysis were performed. Morphological and molecular biological identification suggested that nearly 21 kinds of freshwater mollusks were collected during the survey and a total of 21 important species of molluscs in Xinjiang had been identified, which belongs to 4 orders, 10 families, 14 genus, which belongs to gastropoda and freshwater bivalve mollusks(Bivalvia), respectively. Only one family, one species of shellfish belongs to the bivalvia. Moreover, freshwater snails belongs to gastropods accounted for 9 families and 13 genera in which there was one species of Viviparidae,Bithyniidae,Bradybaenidae,Succineidae,and Hygromiidae and Unionidae,2 Species of Planorbidae and Physidae,7 Species of Lymnaeidae,4 Species of Camaenidae.
In order to investigate the geographical distribution and species of freshwater mollusks, morphological, anatomical and molecular biological techniques were used to identify and classify freshwater mollusks collected from 12 areas of Xinjiang. COI and ITS2 gene fragments were amplified respectively by PCR with specific primers, cloned and sequenced. The molecular identification and phylogenetic analysis were performed. Morphological and molecular biological identification suggested that nearly 21 kinds of freshwater mollusks were collected during the survey and a total of 21 important species of molluscs in Xinjiang had been identified, which belongs to 4 orders, 10 families, 14 genus, which belongs to gastropoda and freshwater bivalve mollusks(Bivalvia), respectively. Only one family, one species of shellfish belongs to the bivalvia. Moreover, freshwater snails belongs to gastropods accounted for 9 families and 13 genera in which there was one species of Viviparidae,Bithyniidae,Bradybaenidae,Succineidae,and Hygromiidae and Unionidae,2 Species of Planorbidae and Physidae,7 Species of Lymnaeidae,4 Species of Camaenidae.
引文
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[3] 王时伟,何良军,杨智秀.塔里木河上游水域淡水螺种类调查[J].安徽农业科学,2010,38(1):196-198.
[4] 郭阳阳.新疆天山不同采集点琥珀螺属形态和分子性状的比较研究[D].乌鲁木齐:新疆大学,2017.
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[11] 江颖,张仪,郭云海.DNA条形码技术在软体动物分类学中的研究进展[J].中国寄生虫学与寄生虫病杂志,2016,34(1):80-83.
[12] FIORENTINO V,MANGANELLI G,GIUSTI F,et al.Recent expansion and relic survival:Phylogeography of the land snail genus Helix (Mollusca,Gastropoda) from south to north Europe[J].Molecular Phylogenetics & Evolution,2016,98:358-372.
[13] COOK L M,COOK L M.Reflections on molluscan shell polymorphisms[J].Biological Journal of the Linnean Society,2017,121 (4):717-730.
[14] LAWTON S P,LIM R M,DUKES J P,et al.Unravelling the riddle of Radix:DNA barcoding for species identification of freshwater snail intermediate hosts of zoonotic digeneans and estimating their inter-population evolutionary relationships[J].Infection Genetics & Evolution,2015,35:63-74.
[15] BENJAMIND C,TIMOTHYJ P,JANEM H.Importance of cryptic species for identifying 'representative' units of biodiversity for freshwater conservation[J].Biological Conservation,2008,141(11):2 821-2 831.
[16] MACHER J N,SALIS R K,BLAKEMORE K S,et al.Multiple-stressor effects on stream invertebrates:DNA barcoding reveals contrasting responses of cryptic mayfly species[J].Ecological Indicators,2016,61(2):159-169.
[17] WEISS M,WEIGAND H,WEIGAND A M,et al.Genome-wide single-nucleotide polymorphism data reveal cryptic species within cryptic freshwater snail species-The case of the Ancylus fluviatilis species complex[J].Ecology & Evolution,2018,8(2):1 063-1 072.
[18] ZEINEDDINE R,DJUIKWOTEUKENG F F,ALJAWHAR I M,et al.Phylogeny of seven Bulinus species originating from endemic areas in three African countries,in relation to the human blood fluke Schistosoma haematobium[J].BMC Evolutionary Biology,2014,14(1):271-271.
[19] VINARSKI M V,AKSENOVA O V,BESPALAYA Y V,et al.Radix dolgini:The integrative taxonomic approach supports the species status of a Siberian endemic snail (Mollusca,Gastropoda,Lymnaeidae)[J].Comptes Rendus Biologies,2016,339 (1):24-36.
[20] KANE R A,STOTHARD J R,EMERY A M,et al.Molecular characterization of freshwater snails in the genus Bulinus:a role for barcodes?[J].Parasites & Vectors,2008,1 (1):1-15.
[21] MANLIK O,SCHMID-HEMPEL R,SCHMID-HEMPEL P.Parasite infection of specific host genotypes relates to changes in prevalence in two natural populations of bumblebees[J].Infection Genetics & Evolution,2017,56:125-132.
[22] COUSTAU C,GOURBAL B,DUVAL D,et al.Advances in gastropod immunity from the study of the interaction between the snail Biomphalaria glabrata and its parasites:A review of research progress over the last decade[J].Fish Shellfish Immunol,2015,46(1):5-16.
[23] JUTHARAT K,SATTRACHAI P,JIRAPORN R,et al.DNA Barcode Identification of Freshwater Snails in the Family Bithyniidae from Thailand[J].Plos One,2013,8(11):e79144.
[24] RAPADO L N,PINHEIRO A D S,FOKOUE H H,et al.Schistosomiasis Control Using Piplartine against Biomphalaria glabrata at Different Developmental Stages[J].PLoS Neglected Tropical Diseases,2013,7(6):e2251.
[25] FARIA R X,ROCHA L M,SOUZA E,et al.Molluscicidal activity of Manilkara subsericea (Mart.) dubard on Biomphalaria glabrata (Say,1818)[J].Acta Tropica,2018,178:163.
[2] 陈德牛,高家祥.中国经济动物志:陆生软体动物[M].北京:科学出版社,1987.
[3] 王时伟,何良军,杨智秀.塔里木河上游水域淡水螺种类调查[J].安徽农业科学,2010,38(1):196-198.
[4] 郭阳阳.新疆天山不同采集点琥珀螺属形态和分子性状的比较研究[D].乌鲁木齐:新疆大学,2017.
[5] 齐钟彦,马绣同.中国动物图谱,软体动物(第四册)[M].北京:科学出版社,1985.
[6] FOLMER O,BLACK M,HOEH W,et al.DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates[J].Mol Mar Biol Biotechnol,1994,3(5):294-299.
[7] KANE RA,ROLLINSON D.Repetitive sequences in the ribosomal DNA internal transcribed spacer of Schistosoma haematobium,Schistosoma intercalatum and Schistosoma mattheei[J].Molecular & Biochemical Parasitology,1994,63(1):153-156.
[8] NAGHAVI M,ABAJOBIR AA,ABBAFATI C,et al.Global,regional,and national age-sex specific mortality for 264 causes of death,1980-2016:a systematic analysis for the Global Burden of Disease Study 2016[J].The Lancet,2017,390(10100):1 151-1 210.
[9] MASCOMA S,VALERO M A,BARGUES M D.Chapter 2 Fasciola,Lymnaeids and Human Fascioliasis,with a Global Overview on Disease Transmission,Epidemiology,Evolutionary Genetics,Molecular Epidemiology and Control [J].Advances in Parasitology,2009,69:41-146.
[10] CORREA A C,ESCOBAR J S,NOYA O,et al.Morphological and molecular characterization of Neotropic Lymnaeidae (Gastropoda:Lymnaeoidea),vectors of fasciolosis [J].Infection Genetics & Evolution,2011,11(8):1 978-1 988.
[11] 江颖,张仪,郭云海.DNA条形码技术在软体动物分类学中的研究进展[J].中国寄生虫学与寄生虫病杂志,2016,34(1):80-83.
[12] FIORENTINO V,MANGANELLI G,GIUSTI F,et al.Recent expansion and relic survival:Phylogeography of the land snail genus Helix (Mollusca,Gastropoda) from south to north Europe[J].Molecular Phylogenetics & Evolution,2016,98:358-372.
[13] COOK L M,COOK L M.Reflections on molluscan shell polymorphisms[J].Biological Journal of the Linnean Society,2017,121 (4):717-730.
[14] LAWTON S P,LIM R M,DUKES J P,et al.Unravelling the riddle of Radix:DNA barcoding for species identification of freshwater snail intermediate hosts of zoonotic digeneans and estimating their inter-population evolutionary relationships[J].Infection Genetics & Evolution,2015,35:63-74.
[15] BENJAMIND C,TIMOTHYJ P,JANEM H.Importance of cryptic species for identifying 'representative' units of biodiversity for freshwater conservation[J].Biological Conservation,2008,141(11):2 821-2 831.
[16] MACHER J N,SALIS R K,BLAKEMORE K S,et al.Multiple-stressor effects on stream invertebrates:DNA barcoding reveals contrasting responses of cryptic mayfly species[J].Ecological Indicators,2016,61(2):159-169.
[17] WEISS M,WEIGAND H,WEIGAND A M,et al.Genome-wide single-nucleotide polymorphism data reveal cryptic species within cryptic freshwater snail species-The case of the Ancylus fluviatilis species complex[J].Ecology & Evolution,2018,8(2):1 063-1 072.
[18] ZEINEDDINE R,DJUIKWOTEUKENG F F,ALJAWHAR I M,et al.Phylogeny of seven Bulinus species originating from endemic areas in three African countries,in relation to the human blood fluke Schistosoma haematobium[J].BMC Evolutionary Biology,2014,14(1):271-271.
[19] VINARSKI M V,AKSENOVA O V,BESPALAYA Y V,et al.Radix dolgini:The integrative taxonomic approach supports the species status of a Siberian endemic snail (Mollusca,Gastropoda,Lymnaeidae)[J].Comptes Rendus Biologies,2016,339 (1):24-36.
[20] KANE R A,STOTHARD J R,EMERY A M,et al.Molecular characterization of freshwater snails in the genus Bulinus:a role for barcodes?[J].Parasites & Vectors,2008,1 (1):1-15.
[21] MANLIK O,SCHMID-HEMPEL R,SCHMID-HEMPEL P.Parasite infection of specific host genotypes relates to changes in prevalence in two natural populations of bumblebees[J].Infection Genetics & Evolution,2017,56:125-132.
[22] COUSTAU C,GOURBAL B,DUVAL D,et al.Advances in gastropod immunity from the study of the interaction between the snail Biomphalaria glabrata and its parasites:A review of research progress over the last decade[J].Fish Shellfish Immunol,2015,46(1):5-16.
[23] JUTHARAT K,SATTRACHAI P,JIRAPORN R,et al.DNA Barcode Identification of Freshwater Snails in the Family Bithyniidae from Thailand[J].Plos One,2013,8(11):e79144.
[24] RAPADO L N,PINHEIRO A D S,FOKOUE H H,et al.Schistosomiasis Control Using Piplartine against Biomphalaria glabrata at Different Developmental Stages[J].PLoS Neglected Tropical Diseases,2013,7(6):e2251.
[25] FARIA R X,ROCHA L M,SOUZA E,et al.Molluscicidal activity of Manilkara subsericea (Mart.) dubard on Biomphalaria glabrata (Say,1818)[J].Acta Tropica,2018,178:163.
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