不同浓度的外源NO对刺五加种子激素及酶含量变化的影响
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摘要
目的:研究外源NO对刺五加种子解除休眠及萌发过程中内源激素及酶的变化规律,为打破刺五加种子休眠和人工栽培提供依据。方法:考察不同浓度(1,5,10,20 mmol·L~(-1))的外源NO供体硝普钠(SNP)处理刺五加种子,而后进行变温层积处理。采用高效液相色谱法检测出在不同层积时间(0,30,50,80,100,130 d)的内源激素赤霉素(GA3),脱落酸(ABA),吲哚乙酸(IAA),吲哚丁酸(IBA)及水杨酸(SA)含量变化。采用酶标仪全程监测体内酶[过氧化氢酶(CAT),超氧化物歧化酶(SOD),过氧化物酶(POD),丙二醛(MDA)]水平的变化。结果:在刺五加种子萌发过程中,GA3,IAA,IBA和SA的含量逐渐增大,脱落酸的含量逐渐减小。POD和MDA水平显著下降,CAT和SOD的酶活力显著上升。外源NO可以提高刺五加种子萌发率,缩短种子萌发时间,20 mmol·L~(-1)SNP促进种子萌发效果最为明显,10 mmol·L~(-1)SNP促进种子萌发效果最弱,呈"V"型变化。结论:SNP促进刺五加种子萌发可能通过提高种子萌发过程中的激素和酶的含量,以提高种子内源NO含量而实现。
Objective: To investigate the effect of exogenous nitric oxide( NO) on breaking the dormancy of Acanthopanax senticosus seeds and the changes in endogenous hormones and enzymes,and provide a basis for breaking the dormancy as well as artificial cultivation of A. senticosus seeds. Method: Different concentrations( 1,5,10,20 mmol·L~(-1)) of sodium nitroprusside( NO donor) were used to treat the A. senticosus seeds,and then thermophilic stratification was conducted. The content changes of endogenous hormones such as gibberellin( GA3),abscisic acid( ABA),indolo acetic acid( IAA),indolo butyric acid( IBA) and salicylic acid( SA) at different stratification time( 0,30,50,80,100,130 d) were tested by high performance liquid chromatography( HPLC).The activity change of its in vivo enzymes [catalase( CAT),superoxide dismutase( SOD),peroxidase( POD),and malondialdehyde( MDA) ]were tested by enzyme-labeled instrument. Result: In the seed germination process of A. senticosus,the contents of GA3,IAA,IBA,and SA were increased gradually,while the content of ABA was reduced gradually. The enzyme activities of POD and MDA were significantly reduced, and the enzyme activities of CAT and SOD were increased obviously. Exogenous NO could increase the seed germination rate and shorten the seed germination time. The effect of 20 mmol·L~(-1) sodium nitroprusside showed the most obvious effect and 10 mmol·L~(-1) SNP showed the weakest effect in promoting seed germination,showing an obvious "V"shape for changes. Conclusion: Sodium nitroprusside could promote the seed germination effect of A. senticosus,probably by increasing the content of hormones and enzyme in the stage of seed germination and improving the contents of endogenous NO during germination.
Objective: To investigate the effect of exogenous nitric oxide( NO) on breaking the dormancy of Acanthopanax senticosus seeds and the changes in endogenous hormones and enzymes,and provide a basis for breaking the dormancy as well as artificial cultivation of A. senticosus seeds. Method: Different concentrations( 1,5,10,20 mmol·L~(-1)) of sodium nitroprusside( NO donor) were used to treat the A. senticosus seeds,and then thermophilic stratification was conducted. The content changes of endogenous hormones such as gibberellin( GA3),abscisic acid( ABA),indolo acetic acid( IAA),indolo butyric acid( IBA) and salicylic acid( SA) at different stratification time( 0,30,50,80,100,130 d) were tested by high performance liquid chromatography( HPLC).The activity change of its in vivo enzymes [catalase( CAT),superoxide dismutase( SOD),peroxidase( POD),and malondialdehyde( MDA) ]were tested by enzyme-labeled instrument. Result: In the seed germination process of A. senticosus,the contents of GA3,IAA,IBA,and SA were increased gradually,while the content of ABA was reduced gradually. The enzyme activities of POD and MDA were significantly reduced, and the enzyme activities of CAT and SOD were increased obviously. Exogenous NO could increase the seed germination rate and shorten the seed germination time. The effect of 20 mmol·L~(-1) sodium nitroprusside showed the most obvious effect and 10 mmol·L~(-1) SNP showed the weakest effect in promoting seed germination,showing an obvious "V"shape for changes. Conclusion: Sodium nitroprusside could promote the seed germination effect of A. senticosus,probably by increasing the content of hormones and enzyme in the stage of seed germination and improving the contents of endogenous NO during germination.
引文
[1]温泽林,刘慧英,周艳,等.一氧化氮参与外源谷胱甘肽对盐胁迫下番茄幼苗抗氧化损伤的调控[J].植物生理学报,2018,54(4):607-617.
[2]张燕,王红兰,蒋舜媛,等.不同氮浓度对羌活幼苗生长及抗氧化酶系统的效应[J].中国实验方剂学杂志,2018,24(7):38-44.
[3]李梅香,李海明,宋发军,等.外源一氧化氮对七叶一枝花种子萌发的影响[J].安徽农业科学,2018,46(5):58-61,65.
[4]王晓晶,呼凤兰,褚盼盼.外源NO对干旱胁迫下大豆种子的萌发及幼苗生长的影响[J].种子,2017,36(2):79-82.
[5]宋要强,屠荫华,惠伟.一氧化氮在打破杜梨种子休眠中的作用[J].四川林业科技,2015,36(5):94-97.
[6]齐永平,顾蔚,罗成,等.华中五味子种子的发育和3种内源激素含量的变化[J].植物生理学通讯,2009,45(6):615-618.
[7] HUANG X S,LIU J H,CHEN X J. Overexpression of Ptr ABF gene,a b ZIP transcription factor isolated from Poncirus trifoliata,enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes[J]. BMC Plant Biol,2010,10(1):230-248.
[8]马伟,梁喜龙,马玲,等.外源一氧化氮供体对黄芪种子萌发的影响[J].东北林业大学学报,2008(1):37-38.
[9]肖承鸿,周涛,江维克,等.低温层积及赤霉素处理对太子参种子萌发与幼苗生长的影响[J].中国实验方剂学杂志,2013,19(15):151-155.
[10]侯姣姣,余仲东,康永祥,等.内生真菌侵染对古侧柏种子萌发及幼苗生长的影响[J].西北林学院学报,2016,31(6):110-115.
[11] GAO Y,ZENG Q,GUO J et al. Genetic characterization reveals no role for the reported ABA receptor,GCR2,in ABA control of seed germination and early seedling development in Arabidopsis[J]. Plant J,2007,52(6):1001-1013.
[12] Gallardo K,Job C,Groot S P C,et al. Proteomics of Arabidopsis seed germination. A comparative study of wild-type and gibberellin-defi-cient seeds[J]. Plant Physiol,2002,129(2):823-837.
[13]秦国臣.内源激素ABA含量、抗氧化物酶活性和种子质量在丝瓜种子发育过程中的变化[D].杭州:浙江大学,2013.
[2]张燕,王红兰,蒋舜媛,等.不同氮浓度对羌活幼苗生长及抗氧化酶系统的效应[J].中国实验方剂学杂志,2018,24(7):38-44.
[3]李梅香,李海明,宋发军,等.外源一氧化氮对七叶一枝花种子萌发的影响[J].安徽农业科学,2018,46(5):58-61,65.
[4]王晓晶,呼凤兰,褚盼盼.外源NO对干旱胁迫下大豆种子的萌发及幼苗生长的影响[J].种子,2017,36(2):79-82.
[5]宋要强,屠荫华,惠伟.一氧化氮在打破杜梨种子休眠中的作用[J].四川林业科技,2015,36(5):94-97.
[6]齐永平,顾蔚,罗成,等.华中五味子种子的发育和3种内源激素含量的变化[J].植物生理学通讯,2009,45(6):615-618.
[7] HUANG X S,LIU J H,CHEN X J. Overexpression of Ptr ABF gene,a b ZIP transcription factor isolated from Poncirus trifoliata,enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes[J]. BMC Plant Biol,2010,10(1):230-248.
[8]马伟,梁喜龙,马玲,等.外源一氧化氮供体对黄芪种子萌发的影响[J].东北林业大学学报,2008(1):37-38.
[9]肖承鸿,周涛,江维克,等.低温层积及赤霉素处理对太子参种子萌发与幼苗生长的影响[J].中国实验方剂学杂志,2013,19(15):151-155.
[10]侯姣姣,余仲东,康永祥,等.内生真菌侵染对古侧柏种子萌发及幼苗生长的影响[J].西北林学院学报,2016,31(6):110-115.
[11] GAO Y,ZENG Q,GUO J et al. Genetic characterization reveals no role for the reported ABA receptor,GCR2,in ABA control of seed germination and early seedling development in Arabidopsis[J]. Plant J,2007,52(6):1001-1013.
[12] Gallardo K,Job C,Groot S P C,et al. Proteomics of Arabidopsis seed germination. A comparative study of wild-type and gibberellin-defi-cient seeds[J]. Plant Physiol,2002,129(2):823-837.
[13]秦国臣.内源激素ABA含量、抗氧化物酶活性和种子质量在丝瓜种子发育过程中的变化[D].杭州:浙江大学,2013.