蓝莓试管苗组培生根技术研究
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摘要
以蓝莓试管苗为材料,筛选适合瓶内生根技术的方法、培养基及培养条件,为蓝莓组培快繁技术提供理论指导.通过在改良的WPM(木本植物培养基)中添加不同浓度的IBA(吲哚丁酸)、NAA(萘乙酸)、活性炭等研究蓝莓试管苗瓶内生根技术,采用正交实验设计,筛选最适蓝莓试管苗瓶内生根培养条件.结果表明:蓝莓试管苗在改良的1/2WPM培养基中生根率高,试管苗的上端和下端对蓝莓的生根率无明显影响,蓝莓生根最适培养基为改良1/2 WPM+IBA 0.8 mg/L+NAA 0.20 mg/L+活性炭0.06%+暗处理14 d,60 d后生根率达83.3%.
Blueberry test-tube seedling was cultured in different culture medium and conditions to explore the appropriate test-tube rooting method and provide theoretical basis for blueberry rapid propagation.IBA,NAA and activated carbon with different concentrations were added into the improved WPM(Woody plant culture medium)and then orthogonal experiment design determined the optimal rooting culture conditions of blueberry in vitro plantlets.The results showed that the rooting rate of blueberry test-tube plantlets was relatively higher in the improved 1/2 WPM medium and the rooting rate was not significantly affected by the upper and lower ends of the test-tube plantlets.The optimal rooting medium of blueberry was improved 1/2 WPM+IBA 0.8 mg/L+NAA 0.2 mg/L+activated carbon 0.06%+dark treatment for 14 days.In this condition,the rooting rate reached 83.3% after 60 days cultivation.
Blueberry test-tube seedling was cultured in different culture medium and conditions to explore the appropriate test-tube rooting method and provide theoretical basis for blueberry rapid propagation.IBA,NAA and activated carbon with different concentrations were added into the improved WPM(Woody plant culture medium)and then orthogonal experiment design determined the optimal rooting culture conditions of blueberry in vitro plantlets.The results showed that the rooting rate of blueberry test-tube plantlets was relatively higher in the improved 1/2 WPM medium and the rooting rate was not significantly affected by the upper and lower ends of the test-tube plantlets.The optimal rooting medium of blueberry was improved 1/2 WPM+IBA 0.8 mg/L+NAA 0.2 mg/L+activated carbon 0.06%+dark treatment for 14 days.In this condition,the rooting rate reached 83.3% after 60 days cultivation.
引文
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[3]姚平,孙书伟.蓝莓组织培养瓶内复壮瓶外生根快繁技术[J].北方园艺,2009(4):161-162.
[4]刘庆忠,赵红军,郑亚芹,等.高灌蓝莓微体繁殖技术研究初报[J].落叶果树,2001(5):1-3.
[5]关丽霞.蓝莓离体培养及植株再生研究[J].广东农业科学,2011(23):47-48.
[6]范梦捷,文光琴,聂飞,等.蓝莓组培苗配套移栽培育技术研究[J].现代农业科技,2010(16):129.
[7]董朝莉.培养基组分对蓝莓组培苗移栽存活的影响[J].广西农业科学,2010,41(6):526-527.
[8]孙阳,刘顺标,程淑云,等. 3个蓝莓品种叶片离体再生及生根技术研究[J].安徽农业科学,2008,36(11):4411-4412.
[9]胡选萍,秦公伟,曹小勇.蓝莓组织培养技术的研究进展[J].分子植物育种,2018,16(3):960-965.
[10]关丽霞,韩德伟.蓝莓组培苗瓶内浅层液体生根技术[J].上海农业科技,2011(3):21-23.
[11]王艳霞,王福玉,张宝刚,等.蓝莓扦插育苗技术[J].中国林副特产,2010,8(4):51-54.
[12]严家驹,黄作喜,李强.蓝莓繁殖技术的研究进展[J].安徽农学通报,2018,24(14):43-46.
[13]孙阳,魏海蓉,程淑云,等.蓝莓组培苗玻璃化及恢复的研究[J].山东农业科学,2008(3):61-63.
[14]王淑珍,来文国,周历萍,等.南高丛蓝莓组培再生技术研究[J].现代农业科技,2011(21):119-120.
[15]程磊,郭伟,陈胜荣,等.蓝莓组织培养技术研究[J].农业科技通讯,2017(12):87-90.
[16]梁文卫,宋鹏慧,阎聪,等.美登蓝莓试管苗瓶内快速生根试验[J].中国果树,2015(4):44-47.
[17]熊翠林.兔眼蓝莓组培苗瓶内生根技术研究[J].安徽农学通报,2018,24(6):46-48.
[18]宋刚,徐银,宋金耀,等.蓝莓试管苗不定根的诱导研究[J].江苏农业科学,2001,39(6):89-91.