基于ITS2序列的“藏茵陈”分子鉴别和遗传多样性研究
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摘要
通过实验测定和GenBank数据库检索印度獐牙菜、川西獐牙菜、大籽獐牙菜、獐牙菜、抱茎獐牙菜、湿生萹蕾、椭圆叶花锚、喉毛花、宿根胁助花、篦齿虎耳草、唐古特虎耳草、山地虎耳草、优越虎耳草等ITS2序列,使用CodonCode Aligner V 5.1和MEGA5.5软件处理ITS2序列,对"藏茵陈"各基原物种变异进行了分析并以Neighbor-Join法构建了"藏茵陈"类各基原物种遗传多样性聚类分析图。结果表明,ITS2序列可以作为"藏茵陈"基原植物的分子鉴别和遗传多样性分析的可靠方法。
Experimentally determination and GenBank database were used to search for their ITS2 se?quences of Swertia chirayita,Swertia mussotii,Swertia franchetiana,Swertia macrosperma,Swertia bimaculata,Gentianopsis paludosa,Halenia elliptica,Comastoma pulmonarium,Lomatogonium perenne and Saxifraga um?bellulata var. pectinata.The softwares of CodonCode Aligner V 5.1 and MEGA 5.5 were used to process the se?quences and the variability of each primordial species of Tibet capillary artemisia were analyzed.The cluster dendrogram of genetic diversity was established by using Neighbor-Join method.The results showed that the ITS2 sequences can be used as a reliable method for the molecular identification and genetic diversity analysis of the Tibet capillary artemisia primordial plant of Tibet capillary artemisia.
Experimentally determination and GenBank database were used to search for their ITS2 se?quences of Swertia chirayita,Swertia mussotii,Swertia franchetiana,Swertia macrosperma,Swertia bimaculata,Gentianopsis paludosa,Halenia elliptica,Comastoma pulmonarium,Lomatogonium perenne and Saxifraga um?bellulata var. pectinata.The softwares of CodonCode Aligner V 5.1 and MEGA 5.5 were used to process the se?quences and the variability of each primordial species of Tibet capillary artemisia were analyzed.The cluster dendrogram of genetic diversity was established by using Neighbor-Join method.The results showed that the ITS2 sequences can be used as a reliable method for the molecular identification and genetic diversity analysis of the Tibet capillary artemisia primordial plant of Tibet capillary artemisia.
引文
[1]藏医药经典文献集成编委会.晶珠本草[M].上海:上海科学技术出版社,1988,79-80.Compilation committee of classic documents of Tibetan medicine.Shel gong shel phreng[M].Shanghai:Shanghai Scientific and Technical Publishers,1988,79-80.
[2]钟国跃,古锐,周华蓉,等.藏药“蒂达”的名称与品种考证[J].中国中药杂志,2009,34(23):3139-3144.Zhong G Y,Gu R,Zhou H R,et al.Textual criticized and collated on names and breeds of Tibetan medicine“Dida”[J].China Journal of Chinese Materia Medica,2009,34(23):3139-3144.
[3]费曜,钟国跃,刘翔,等.常用藏药“松蒂”(篦齿虎耳草)的显微鉴别研究[J].中国中药杂志,2013,38(6):902-908.Fei Y,Zhong G Y,Liu X,et al.Microscopic identification of Tibetan medicinal herb“Songdi”(Saxifraga umbellulata var.pectinate)[J].China Journal of Chinese Materia Medica,2013,38(6):902-908.
[4]马俊蓉,李子信,温红艳,等.“藏茵陈”的基原植物和药用亲缘学[J].广西植物,2009,29(4):552-555.Ma J R,Li Z X,Wen H Y,et al.Identification of the original plant of Tibetan material medica“Zangyinchen”[J].Guihaia,2009,29(4):552-555.
[5]Ji H P,Kim Y H,Ham C S,et al.Molecular identification of forensically important calliphoridae and sarcophagidae species using ITS2 nucleotide sequences[J].Forensic Science International,2018,284:1-4.
[6]Li Q,Xu J G,Han L,et al.Evaluation of ITS2 for intraspecific identification of Paeonia lactiflora cultivars[J].Biotechnology Reports,2017,15:101-106.
[7]李永波,吴波,幕泽迳,等.5种藏医药用翠雀属植物的ITS2分子鉴别研究[J].江西农业大学学报,2015,37(6):1100-1104.Li Y B,Wu B,Mu Z J,et al.An ITS2 sequence analysis of five tibetan medicine plants of delphinium L.[J].Acta Agriculturae Universitatis Jiangxiensis,2015,37(6):1100-1104.
[8]高光跃,李鸣,冯毓秀,等.11种獐牙菜及近缘植物中有效成分的高效液相色谱测定[J].药学学报,1994,29(12):910-914.Gao G Y,Li M,Feng Y X,et al.Determination of effective constituents in 11 Swertia and related plants by HPLC[J].Acta Pharmaceutica Sinica,1994,29(12):910-914.
[9]赵纪峰.藏茵陈的资源调查与生药学研究[D].北京:北京中医药大学,2008.Zhao J F.Resources of investigation and study on pharmacognosy of“Zangyinchen”[D].Beijing:Beijing University of Chines Medicin,2008.
[10]费曜.藏药“蒂达(藏茵陈)”品种之一蓖齿虎耳草的品质研究[D].成都:成都中医药大学,2012.Fei Y.Study on variety classification of tibetan medicinal herb“Songdi”and variety and quality of Saxifraga umbellulata var.Pectinata[D].Chengdu:Chengdu University of Traditional Chinese Medicine,2012.
[11]刘海涛.五味子科药用植物亲缘学初探及两种五味子科药用植物化学成分的研究[D].北京:中国协和医科大学,2009.Liu H T.A Preliminary pharmacophylogenetic investigation of the Schisandraceae family and studies on the chemical constituents of two medicinal species[D].Beijing:Peking Union Medical College,2009.
[12]何春年.芍药属药用植物亲缘学研究[D].北京:中国协和医科大学,2010.He C N.Study on the pharmacophylogentic significance of the Paeonia in China[D].Beijing:Peking Union Medical College,2010.
[13]彭冰.三白草的化学成分研究及三白草科药用植物亲缘学初探[D].北京:北京协和医学院,2011.Peng B.Studies on the chemical constituents of Saururus chinensis and a preliminary pharmacophylogenetic investigation of Saururaceae[D].Beijing:Peking Union Medical College,2011.
[14]曹聪梅.中国金粟兰科植物药用亲缘学研究[D].北京:中国协和医科大学,2009.Cao C M.Study on the pharmcophylogenetic significance of the Chloranthaceae in China[D].Beijing:Peking Union Medical College,2009.
[15]刘黄刚,张铁军,王莉丽,等.獐牙菜属药用植物亲缘关系及其资源评价[J].中草药,2011,42(8):1646-1650.Liu H G,Zhang T J,Wang L L,et al.Gentic relationship and resources evaluation of medicinal plants in Swertia L.[J].Chinese Traditional and Herbal Drugs,2011,42(8):1646-1650.
[2]钟国跃,古锐,周华蓉,等.藏药“蒂达”的名称与品种考证[J].中国中药杂志,2009,34(23):3139-3144.Zhong G Y,Gu R,Zhou H R,et al.Textual criticized and collated on names and breeds of Tibetan medicine“Dida”[J].China Journal of Chinese Materia Medica,2009,34(23):3139-3144.
[3]费曜,钟国跃,刘翔,等.常用藏药“松蒂”(篦齿虎耳草)的显微鉴别研究[J].中国中药杂志,2013,38(6):902-908.Fei Y,Zhong G Y,Liu X,et al.Microscopic identification of Tibetan medicinal herb“Songdi”(Saxifraga umbellulata var.pectinate)[J].China Journal of Chinese Materia Medica,2013,38(6):902-908.
[4]马俊蓉,李子信,温红艳,等.“藏茵陈”的基原植物和药用亲缘学[J].广西植物,2009,29(4):552-555.Ma J R,Li Z X,Wen H Y,et al.Identification of the original plant of Tibetan material medica“Zangyinchen”[J].Guihaia,2009,29(4):552-555.
[5]Ji H P,Kim Y H,Ham C S,et al.Molecular identification of forensically important calliphoridae and sarcophagidae species using ITS2 nucleotide sequences[J].Forensic Science International,2018,284:1-4.
[6]Li Q,Xu J G,Han L,et al.Evaluation of ITS2 for intraspecific identification of Paeonia lactiflora cultivars[J].Biotechnology Reports,2017,15:101-106.
[7]李永波,吴波,幕泽迳,等.5种藏医药用翠雀属植物的ITS2分子鉴别研究[J].江西农业大学学报,2015,37(6):1100-1104.Li Y B,Wu B,Mu Z J,et al.An ITS2 sequence analysis of five tibetan medicine plants of delphinium L.[J].Acta Agriculturae Universitatis Jiangxiensis,2015,37(6):1100-1104.
[8]高光跃,李鸣,冯毓秀,等.11种獐牙菜及近缘植物中有效成分的高效液相色谱测定[J].药学学报,1994,29(12):910-914.Gao G Y,Li M,Feng Y X,et al.Determination of effective constituents in 11 Swertia and related plants by HPLC[J].Acta Pharmaceutica Sinica,1994,29(12):910-914.
[9]赵纪峰.藏茵陈的资源调查与生药学研究[D].北京:北京中医药大学,2008.Zhao J F.Resources of investigation and study on pharmacognosy of“Zangyinchen”[D].Beijing:Beijing University of Chines Medicin,2008.
[10]费曜.藏药“蒂达(藏茵陈)”品种之一蓖齿虎耳草的品质研究[D].成都:成都中医药大学,2012.Fei Y.Study on variety classification of tibetan medicinal herb“Songdi”and variety and quality of Saxifraga umbellulata var.Pectinata[D].Chengdu:Chengdu University of Traditional Chinese Medicine,2012.
[11]刘海涛.五味子科药用植物亲缘学初探及两种五味子科药用植物化学成分的研究[D].北京:中国协和医科大学,2009.Liu H T.A Preliminary pharmacophylogenetic investigation of the Schisandraceae family and studies on the chemical constituents of two medicinal species[D].Beijing:Peking Union Medical College,2009.
[12]何春年.芍药属药用植物亲缘学研究[D].北京:中国协和医科大学,2010.He C N.Study on the pharmacophylogentic significance of the Paeonia in China[D].Beijing:Peking Union Medical College,2010.
[13]彭冰.三白草的化学成分研究及三白草科药用植物亲缘学初探[D].北京:北京协和医学院,2011.Peng B.Studies on the chemical constituents of Saururus chinensis and a preliminary pharmacophylogenetic investigation of Saururaceae[D].Beijing:Peking Union Medical College,2011.
[14]曹聪梅.中国金粟兰科植物药用亲缘学研究[D].北京:中国协和医科大学,2009.Cao C M.Study on the pharmcophylogenetic significance of the Chloranthaceae in China[D].Beijing:Peking Union Medical College,2009.
[15]刘黄刚,张铁军,王莉丽,等.獐牙菜属药用植物亲缘关系及其资源评价[J].中草药,2011,42(8):1646-1650.Liu H G,Zhang T J,Wang L L,et al.Gentic relationship and resources evaluation of medicinal plants in Swertia L.[J].Chinese Traditional and Herbal Drugs,2011,42(8):1646-1650.
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