赤潮藻类在碳源受限生长过程中培养液pH的变化规律
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摘要
本实验以赤潮藻中肋骨条藻、赤潮异弯藻和塔玛亚历山大藻作为实验藻种,在碳源不足的环境条件下进行培养,测定生物量和pH等监测指标,探讨了在正常营养盐、缺氮营养盐和缺磷营养盐条件下藻类在不同生长时期pH的变化情况。实验结果表明,赤潮藻在碳源受限的生长环境下,藻液pH的增加量比碳源正常环境下要高,其中中肋骨条藻在正常、缺氮和缺磷3种营养盐培养下pH分别增加了1. 4、1. 5和1. 4;赤潮异弯藻在正常、缺氮和缺磷3种营养盐培养下pH分别增加了1. 2、1. 1和1. 0;塔玛亚历山大藻在正常、缺氮和缺磷3种营养盐培养下pH分别增加了1. 6、1. 6和1. 8。pH与细胞数量之间存在显著性正相关,与磷酸盐浓度之间存在显著性负相关。
Skeletonema costatum,Hereosigam akashiwo and Alexandrium tamareme were used to investigate the impact of different nutritional conditions on growth under restricted CO2 conditions. Biomass,pH and PO4-P were measured to discuss how carbon source and different nutrition conditions effect the change of pH during the different growth phases. The results showed that the increase of pH in the carbon restrict system was higher than that in the no carbon restrict system. The pH of Skeletonema costatum in normal,nitrogen and phosphorus deficiencies increased by 1. 4,1. 5 and 1. 4,respectively. The pH of Hereosigam akashiwo increased by 1. 2,1. 1 and 1. 0,respectively. The pH of Alexandrium tamarense increased by 1. 6,1. 6 and 1. 8,respectively. The pH and biomass had a significant positive correlation; the pH and phosphate had a significant negative correlation.
Skeletonema costatum,Hereosigam akashiwo and Alexandrium tamareme were used to investigate the impact of different nutritional conditions on growth under restricted CO2 conditions. Biomass,pH and PO4-P were measured to discuss how carbon source and different nutrition conditions effect the change of pH during the different growth phases. The results showed that the increase of pH in the carbon restrict system was higher than that in the no carbon restrict system. The pH of Skeletonema costatum in normal,nitrogen and phosphorus deficiencies increased by 1. 4,1. 5 and 1. 4,respectively. The pH of Hereosigam akashiwo increased by 1. 2,1. 1 and 1. 0,respectively. The pH of Alexandrium tamarense increased by 1. 6,1. 6 and 1. 8,respectively. The pH and biomass had a significant positive correlation; the pH and phosphate had a significant negative correlation.
引文
[1]关道明,战秀文.我国沿海水域赤潮灾害及其防治对策[J].海洋环境科学,2003,22(2):60-63.
[2]HOBSON L A,HANSON C E,HOLETON C. An ecological basisfor extracellular carbonic anhydrase in marine unicellularalgae[J]. Journal of Phycology,2001,37(5):717-723.
[3] TARALDSVIK M,MYKLESTAD S. The effect of pH on growthrate,biochemical composition and extracellular carbohydrate pro-duction of the marine diatom Skeletonema costatum[J]. EuropeanJournal of Phycology,2000,35(2):189-194.
[4]石晓勇,王修林,陆茸,等.东海赤潮高发区春季溶解氧和pH分布特征及影响因素探讨[J].海洋与湖沼,2005,36(5):404-412.
[5]GENSEMER R W,SMITH R E H,DUTHIE H C,et al. pH toler-ance and metal toxicity in populations of the planktonic diatomAsterionella:influences of synthetic and natural dissolved organiccarbon[J]. Canadian Journal of Fisheries and Aquatic Sciences,1993,50(1):121-132.
[6]DONEY S C,FABRY V J,FEELY R A,et al. Ocean acidifica-tion:the other CO2problem[J]. Annual Review of Marine Sci-ence,2009,1:169-192.
[7]陈雄文,高坤山.赤潮藻中肋骨条藻的光合作用对海水pH和N变化的响应[J].水生生物学报,2004,28(6):635-639.
[8]MACEDO M F,DUARTE P,MENDES P,et al. Annual variationof environmental variables,phytoplankton species composition andphotosynthetic parameters in a coastal lagoon[J]. Journal ofPlankton Research,2001,23(7):719-732.
[9]HINGA K R. Effects of pH on coastal marine phytoplankton[J].Marine Ecology Progress Series,2002,238:281-300.
[10]石岩峻,胡晗华,马润宇,等.塔玛亚历山大藻对氮和磷的吸收及其生长特性[J].应用生态学报,2003,14(7):1143-1146.
[11]吴玉霖,周成旭.甲藻赤潮的海洋环境危害及其防治[J].海洋环境科学,1997,16(4):58-63.
[12]HANSEN P J. Effect of high pH on the growth and survival ofmarine phytoplankton:implications for species succession[J].Aquatic Microbial Ecology,2002,28(3):279-288.
[13]程军利,张鹰,张东,等.海州湾赤潮发生期生态环境要素分析[J].海洋科学进展,2009,27(2):217-223.
[14]卢敏,张龙军,李超,等. 1999年7月胶州湾东部赤潮生消过程生态环境要素分析[J].黄渤海海洋,2001,19(4):43-50.
[15]汪芳,葛蔚,柴超,等.不同营养条件对东海原甲藻和中肋骨条藻竞争参数的影响[J].应用生态学报,2012,23(5):1393-1399.
[16]SUKENIK A,TCHERNOV D,KAPLAN A,et al. Uptake,efflux,and photosynthetic utilization of inorganic carbon by the marineeustigmatophyte Nannochloropsis sp.[J]. Journal of Phycology,1997,33(6):969-974.
[17]霍文毅,俞志明,邹景忠,等.胶州湾中肋骨条藻赤潮与环境因子的关系[J].海洋与湖沼,2001,32(3):311-318.
[18]IBELINGS B W,MABERLY S C. Photoinhibition and the availa-bility of inorganic carbon restrict photosynthesis by surfaceblooms of cyanobacteria[J]. Limnology and Oceanography,1998,43(3):408-419.
[19]李晓琳.赤潮生消过程中赤潮藻类稳定同位素组成变化[D].大连:大连海事大学,2015.
[20]庄万金.厦门西海域赤潮发生区海水pH的分布及与赤潮的关系[J].海洋环境科学,1992,11(3):64-70.
[21]赖利J P,斯基罗G.化学海洋学-第二卷[M].崔清晨,钱佐国,唐思齐,译.北京:海洋出版社,1982:464.
[22]BOTO K G,BUNT J S. Dissolved oxygen and pH relationships innorthern Australian mangrove waterways[J]. Limnology and O-ceanography,1981,26(6):1176-1178.
[23]蔡燕红,蒋晓山,黄秀清.舟山海域一次具齿原甲藻赤潮初探[J].海洋环境科学,2002,21(1):42-45.
[24]蔡燕红,项有堂.舟山海域具齿原甲藻赤潮初探[J].海洋环境科学,2002,21(4):34-36.
[25]ROST B,RICHTER K U,RIEBESELL U,et al. Inorganic carbonacquisition in red tide dinoflagellates[J]. Plant,Cell&Environ-ment,2006,29(5):810-822.
[26]GAO K S,ARUGA Y,ASADA K,et al. Enhanced growth of thered alga Porphyra yezoensis Ueda in high CO2concentrations[J]. Journal of Applied Phycology,1991,3(4):355-362.
[27]王玉衡,蒋国昌,董恒霖.春季浙江南部海区溶解氧、pH值和营养盐分布特征及相互关系研究[J].海洋学报,1990,12(5):654-660.
[2]HOBSON L A,HANSON C E,HOLETON C. An ecological basisfor extracellular carbonic anhydrase in marine unicellularalgae[J]. Journal of Phycology,2001,37(5):717-723.
[3] TARALDSVIK M,MYKLESTAD S. The effect of pH on growthrate,biochemical composition and extracellular carbohydrate pro-duction of the marine diatom Skeletonema costatum[J]. EuropeanJournal of Phycology,2000,35(2):189-194.
[4]石晓勇,王修林,陆茸,等.东海赤潮高发区春季溶解氧和pH分布特征及影响因素探讨[J].海洋与湖沼,2005,36(5):404-412.
[5]GENSEMER R W,SMITH R E H,DUTHIE H C,et al. pH toler-ance and metal toxicity in populations of the planktonic diatomAsterionella:influences of synthetic and natural dissolved organiccarbon[J]. Canadian Journal of Fisheries and Aquatic Sciences,1993,50(1):121-132.
[6]DONEY S C,FABRY V J,FEELY R A,et al. Ocean acidifica-tion:the other CO2problem[J]. Annual Review of Marine Sci-ence,2009,1:169-192.
[7]陈雄文,高坤山.赤潮藻中肋骨条藻的光合作用对海水pH和N变化的响应[J].水生生物学报,2004,28(6):635-639.
[8]MACEDO M F,DUARTE P,MENDES P,et al. Annual variationof environmental variables,phytoplankton species composition andphotosynthetic parameters in a coastal lagoon[J]. Journal ofPlankton Research,2001,23(7):719-732.
[9]HINGA K R. Effects of pH on coastal marine phytoplankton[J].Marine Ecology Progress Series,2002,238:281-300.
[10]石岩峻,胡晗华,马润宇,等.塔玛亚历山大藻对氮和磷的吸收及其生长特性[J].应用生态学报,2003,14(7):1143-1146.
[11]吴玉霖,周成旭.甲藻赤潮的海洋环境危害及其防治[J].海洋环境科学,1997,16(4):58-63.
[12]HANSEN P J. Effect of high pH on the growth and survival ofmarine phytoplankton:implications for species succession[J].Aquatic Microbial Ecology,2002,28(3):279-288.
[13]程军利,张鹰,张东,等.海州湾赤潮发生期生态环境要素分析[J].海洋科学进展,2009,27(2):217-223.
[14]卢敏,张龙军,李超,等. 1999年7月胶州湾东部赤潮生消过程生态环境要素分析[J].黄渤海海洋,2001,19(4):43-50.
[15]汪芳,葛蔚,柴超,等.不同营养条件对东海原甲藻和中肋骨条藻竞争参数的影响[J].应用生态学报,2012,23(5):1393-1399.
[16]SUKENIK A,TCHERNOV D,KAPLAN A,et al. Uptake,efflux,and photosynthetic utilization of inorganic carbon by the marineeustigmatophyte Nannochloropsis sp.[J]. Journal of Phycology,1997,33(6):969-974.
[17]霍文毅,俞志明,邹景忠,等.胶州湾中肋骨条藻赤潮与环境因子的关系[J].海洋与湖沼,2001,32(3):311-318.
[18]IBELINGS B W,MABERLY S C. Photoinhibition and the availa-bility of inorganic carbon restrict photosynthesis by surfaceblooms of cyanobacteria[J]. Limnology and Oceanography,1998,43(3):408-419.
[19]李晓琳.赤潮生消过程中赤潮藻类稳定同位素组成变化[D].大连:大连海事大学,2015.
[20]庄万金.厦门西海域赤潮发生区海水pH的分布及与赤潮的关系[J].海洋环境科学,1992,11(3):64-70.
[21]赖利J P,斯基罗G.化学海洋学-第二卷[M].崔清晨,钱佐国,唐思齐,译.北京:海洋出版社,1982:464.
[22]BOTO K G,BUNT J S. Dissolved oxygen and pH relationships innorthern Australian mangrove waterways[J]. Limnology and O-ceanography,1981,26(6):1176-1178.
[23]蔡燕红,蒋晓山,黄秀清.舟山海域一次具齿原甲藻赤潮初探[J].海洋环境科学,2002,21(1):42-45.
[24]蔡燕红,项有堂.舟山海域具齿原甲藻赤潮初探[J].海洋环境科学,2002,21(4):34-36.
[25]ROST B,RICHTER K U,RIEBESELL U,et al. Inorganic carbonacquisition in red tide dinoflagellates[J]. Plant,Cell&Environ-ment,2006,29(5):810-822.
[26]GAO K S,ARUGA Y,ASADA K,et al. Enhanced growth of thered alga Porphyra yezoensis Ueda in high CO2concentrations[J]. Journal of Applied Phycology,1991,3(4):355-362.
[27]王玉衡,蒋国昌,董恒霖.春季浙江南部海区溶解氧、pH值和营养盐分布特征及相互关系研究[J].海洋学报,1990,12(5):654-660.