一株异养硝化菌AD-28氨氮去除特性研究
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摘要
以异养硝化菌——嗜碱假单胞菌AD-28为试验对象,通过单因子试验测定好氧条件下碳源、碳氮比、温度、pH、溶解氧、初始氮质量浓度及盐度等不同因素对嗜碱假单胞菌AD-28生长及氨氮去除的影响。试验结果表明,适用于嗜碱假单胞菌AD-28生长和氨氮去除的最佳条件为:碳源柠檬酸钠、丁二酸钠、乙酸钠,碳氮比20,温度25～35℃,pH 6.0,转速120r/min;在最佳条件下,嗜碱假单胞菌AD-28对初始质量浓度为20～160mg/L的氨氮去除率在24h内达95%以上。嗜碱假单胞菌AD-28能直接以氨氮为底物进行高效的硝化作用,并能耐受较低的温度(15℃)和较高的盐度(NaCl质量浓度为50g/L),在调节养殖水体水质方面具有广阔的应用前景。
The effects of carbon source,C/N ratio,temperature,pH,dissolved oxygen,salinity and initial ammonia nitrogen concentration on growth and ammonia nitrogen removal efficiency of Pseudomonas alcaliphila AD-28 as a high efficiency heterogeneous nitrifier were studied under the aerobic conditions in a single-factor experiment.The results showed that the optimum growth and nitrification was observed in AD-28 under conditions of sodium citrate,sodium acetate and sodium succinate as carbon resources,C/N ratio of 20,25—35℃,pH 6.0,and a rotating speed of 120r/min,with ammonia nitrogen removal rate of up to 95%treated by AD-28 for 24hat initial ammonia nitrogen concentration from 20mg/L to 160mg/L.The findings indicated that strain AD-28 exhibited an amazing ability to remove the ammonia nitrogen at low temperature(15℃)and high salt tolerance(NaCl concentration of 50g/L),which has great potential treatment of aquaculture water.
The effects of carbon source,C/N ratio,temperature,pH,dissolved oxygen,salinity and initial ammonia nitrogen concentration on growth and ammonia nitrogen removal efficiency of Pseudomonas alcaliphila AD-28 as a high efficiency heterogeneous nitrifier were studied under the aerobic conditions in a single-factor experiment.The results showed that the optimum growth and nitrification was observed in AD-28 under conditions of sodium citrate,sodium acetate and sodium succinate as carbon resources,C/N ratio of 20,25—35℃,pH 6.0,and a rotating speed of 120r/min,with ammonia nitrogen removal rate of up to 95%treated by AD-28 for 24hat initial ammonia nitrogen concentration from 20mg/L to 160mg/L.The findings indicated that strain AD-28 exhibited an amazing ability to remove the ammonia nitrogen at low temperature(15℃)and high salt tolerance(NaCl concentration of 50g/L),which has great potential treatment of aquaculture water.
引文
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[23]黄廷林,张丽娜,张海涵,等.一株贫营养异养硝化—好氧反硝化菌的筛选及脱氮特性[J].生态环境学报2015,24(1):113-120.
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[25]Lei Y,Wang Y Q,Liu H J,et al.A novel heterotrophic nitrifying and aerobic denitrifying bacterium,Zobellella taiwanensisDN-7, can remove highstrength ammonium[J].Applied Biochemistry and Biotechnology,2016,100(9):4219-4229.
[26]蒋华东,何晓红,张礼霞,等.一株假单胞菌(Pseudomonas alcaliphila MBR)好氧还原亚硒酸钠为红色单质硒[J].微生物学报,2010,50(10):1347-1352.
[27]Seyyed A M,Shaliza I,Mohamed K A.Effect of carbon source on acclimatization of nitrifying bacteria to achieve high-rate partial nitrification of wastewater with high ammonium concentration[J].Applied Water Science,2017,7(1):165-173.
[28]高宇,刘文斌,王莹,等.可用于水体污染控制的氨氮转化菌筛选及部分降解特性的实验研究[J].环境工程学报,2010,4(4):855-860.
[29]王田野,魏荷芬,胡子全,等.一株异养硝化好氧反硝化菌的筛选鉴定及其脱氮特性[J].环境科学学报,2017,37(3):945-953.
[30]王兆阳,陈国耀,姜珂,等.1株耐冷兼性嗜碱好氧反硝化菌的分离鉴定及反硝化特性[J].环境科学,2014,35(6):2341-2348.
[2]胡庚东,宋超,陈家长,等.池塘循环水养殖模式的构建及其对氮磷的去除效果[J].生态与农村环境学报,2011,27(3):82-86.
[3] Guo L Y,Qian K C,Fei F,et al.Application potential of a newly isolated indigenous aerobic denitrifier for nitrate and ammonium removal of eutrophic lake water[J].Bioresource Technology,2013,142(4):45-51.
[4] Zhang X Y,Zhu F,Chen L,et al.Removal of ammonia nitrogen from wastewater using an aerobic cathode microbial fuel cell[J].Bioresource Technology,2013,146(10):161-168.
[5]苟莎,黄钧.异养硝化细菌脱氮特性及研究进展[J].微生物学通报,2009,36(2):255-260.
[6] Quastel J H,Scholefield P G.Influence of organic nitrogen compounds on nitrifications in soil[J].Nature,1949,164(4182):1068-1072.
[7]陈茂霞,王欢,周后珍,等.异养硝化—好氧反硝化菌HN-O2的筛选及其特性[J].应用与环境生物学报,2013,19(4):688-693.
[8]宋宇杰,李屹,刘玉香,等.碳源和氮源对异养硝化好氧反硝化菌株Y1脱氮性能的影响[J].环境科学学报,2013,33(9):2491-2497.
[9]肖继波,江惠霞,褚淑祎.新异养硝化菌Colloides sp.JZ11的鉴定及其硝化性能[J].应用生态学报,2012,23(6):1635-1640.
[10]赵丹,于德爽,李津,等.菌株ZD8的分离鉴定及其异养硝化和缺氧/好氧反硝化特性研究[J].环境科学学报,2013,33(11):3007-3016.
[11]邓斌,郑佳佳,傅罗琴,等.一株高效硝化菌的鉴定及其特性[J].环境工程学报,2013,7(9):3635-3641.
[12]刘天琪,金若菲,周集体,等.异养硝化-好氧反硝化菌ADN-42的脱氮特性[J].环境工程学报,2015,9(2):989-996.
[13]邹艳艳,张宇,李明智,等.一株异养硝化—好氧反硝化细菌的分离鉴定及脱氮活性研究[J].中国环境科学,2016,36(3):887-893.
[14]孙庆花,于德爽,张培玉,等.海洋菌株y3的分离鉴定及其异养硝化—好氧反硝化特性[J].环境科学,2016,37(3):1089-1097.
[15]曾庆梅,司文攻,李志强,等.一株高效异养硝化菌的选育、鉴定及其硝化条件[J].微生物学报,2010,50(6):803-810.
[16]Yang X N,Ye J X,Liu L M,et al.Anaerobic biodegradation of pyrene by Paracoccus denitrificans under various nitrate/nitrite-reducing conditions[J].Water,Air,&Soil Pollution,2013,224(5):1-10.
[17]Joo H S,Hirai M,Shoda M.Piggery wastewater treatment using Alcaligenes faecalis strain No.4with heterotrophic nitrification and aerobic denitrification[J].Water Research,2006,40(16):3029-3036.
[18]方海洋,王智,李建华,等.异养硝化—好氧反硝化菌粪产碱杆菌的脱氮特性[J].环境工程学报,2015,9(2):983-988.
[19]Chen J,Gu S Y,Hao H H,et al.Characteristics and metabolic pathway of Alcaligenes sp.TB for simultaneous heterotrophic nitrification-aerobic denitrification[J].Applied Microbiology and Biotechnology,2016,100(22):9787-9794.
[20]周迎芹,信欣,姚力,等.一株高效异养硝化—好氧反硝化菌的分离鉴定及脱氮性能[J].环境工程学报,2013,7(10):4127-4132.
[21]何腾霞,倪九派,李振轮,等.1株Arthrobacter arilaitensis菌的耐冷异养硝化和好氧反硝化作用[J].环境科学,2016,37(3):1082-1088.
[22]陈昢圳,王立刚,王迎春,等.异养硝化—好氧反硝化菌的筛选及脱氮性能的实验研究[J].环境科学,2009,30(12):3614-3618.
[23]黄廷林,张丽娜,张海涵,等.一株贫营养异养硝化—好氧反硝化菌的筛选及脱氮特性[J].生态环境学报2015,24(1):113-120.
[24]Chen J,Zheng J,Li Y,et al.Characteristics of a novel thermophilic heterotrophic bacterium,Anoxybacillus contaminans HA,for nitrification-aerobic denitrification[J].Applied Biochemistry and Biotechnology,2015,99(24):10695-10702.
[25]Lei Y,Wang Y Q,Liu H J,et al.A novel heterotrophic nitrifying and aerobic denitrifying bacterium,Zobellella taiwanensisDN-7, can remove highstrength ammonium[J].Applied Biochemistry and Biotechnology,2016,100(9):4219-4229.
[26]蒋华东,何晓红,张礼霞,等.一株假单胞菌(Pseudomonas alcaliphila MBR)好氧还原亚硒酸钠为红色单质硒[J].微生物学报,2010,50(10):1347-1352.
[27]Seyyed A M,Shaliza I,Mohamed K A.Effect of carbon source on acclimatization of nitrifying bacteria to achieve high-rate partial nitrification of wastewater with high ammonium concentration[J].Applied Water Science,2017,7(1):165-173.
[28]高宇,刘文斌,王莹,等.可用于水体污染控制的氨氮转化菌筛选及部分降解特性的实验研究[J].环境工程学报,2010,4(4):855-860.
[29]王田野,魏荷芬,胡子全,等.一株异养硝化好氧反硝化菌的筛选鉴定及其脱氮特性[J].环境科学学报,2017,37(3):945-953.
[30]王兆阳,陈国耀,姜珂,等.1株耐冷兼性嗜碱好氧反硝化菌的分离鉴定及反硝化特性[J].环境科学,2014,35(6):2341-2348.
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