不同浓度香蕉汁对金钗石斛组培苗生长的影响
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摘要
[目的]研究不同浓度香蕉汁对金钗石斛组培苗生长的影响。[方法]以金钗石斛无菌组培苗为材料,以MS为基本培养基,研究不同浓度的香蕉汁(0、10、20、30、40 g/L,其中0为对照组)对金钗石斛组培苗生长的影响。[结果]香蕉汁浓度为30 g/L时,金钗石斛组培苗生长速度最快,株高增长最明显,鲜重增加最多,根系粗壮发达,且与对照组相比差异明显。[结论]以30 g/L香蕉汁为培育金钗石斛组培苗的最佳浓度。
[Objective] The research aimed to study the effects of different banana juice concentrations on the growth of Dendrobium nobile tissue culture seedlings.[Method]Sterile tissue culture seedlings of Dendrobium nobile were used as materials,and MS was used as the basic medium to study the effects of different concentrations of banana juice(0,10,20,30,40 g/L,of which 0 was the control group) on the growth of tissue culture seedlings.[Result]When the concentration of banana juice was 30 g/L,the growth rate of the tissue culture seedlings of Dendrobium nobile was the fastest,the growth of plant height was the most obvious,the fresh weight increased most,the root system was thick and developed,and the difference was obvious compared with the control group.
[Objective] The research aimed to study the effects of different banana juice concentrations on the growth of Dendrobium nobile tissue culture seedlings.[Method]Sterile tissue culture seedlings of Dendrobium nobile were used as materials,and MS was used as the basic medium to study the effects of different concentrations of banana juice(0,10,20,30,40 g/L,of which 0 was the control group) on the growth of tissue culture seedlings.[Result]When the concentration of banana juice was 30 g/L,the growth rate of the tissue culture seedlings of Dendrobium nobile was the fastest,the growth of plant height was the most obvious,the fresh weight increased most,the root system was thick and developed,and the difference was obvious compared with the control group.
引文
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[7] 周俊.石斛的历史、分布与功能[J].中国药学杂志,2013,48(19):1609.
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[13] 郑建铃.金钗石斛种苗快繁及林下栽培技术的初步研究[J].福建林业,2015(3):36-38,45.
[14] 徐红,王峥涛,丁家宜,等.药用石斛生物技术的研究概况[J].中国野生植物资源,2001,20(1):1-4.
[15] 吴辉,赵俊,宋锡全.四种不同培养基对诱导金钗石斛幼苗生根的影响[J].贵州科学,2007,25(2):65-67.
[16] 张明,夏鸿西,朱利泉,等.石斛组织培养研究进展[J].中国中药杂志,2000,25(6):323-326.
[17] 彭文书,马莉,赵苗苗,等.铁皮石斛组培苗生根条件优化研究[J].热带农业科学,2015,35(2):13-18.
[18] 索海翠,王科翰,李武,等.铁皮石斛组培苗生长差异与移栽成活率相关性研究[J].广东农业科学,2014(14):20-23.
[19] 熊庆娥.植物生理学实验教程[M].成都:四川科学技术出版社,2003:30-56.
[20] 刘石泉,董萌,赵运林.香蕉对疏花石斛试管苗生长的影响[J].中国林副特产,2009(6):10-12.
[21] 黎建玲,黄肇宇,詹源庆,等.金钗石斛试管苗生根研究[J].广西科学院学报,2006,22(2):87-89.
[22] 魏凤娟.铁皮石斛组织培养与栽培技术研究进展[J].广东农业科学,2010,37(4):81-85.
[23] 姚春,李泽生,李薇莎,等.串珠石斛组织培养与快速繁殖技术研究[J].安徽农业科学,2015,43(30):33-34,69.
[24] 林秀莲,马骁勇.‘阿里山’蝴蝶兰组织培养及其驯化移栽技术[J].亚热带农业研究,2015,11(1):31-34.
[2] 张光浓,毕志明,王峥涛,等.石斛属植物化学成分研究进展[J].中草药,2003,34(6):附5-附8.
[3] 魏小勇.石斛属植物生物碱研究进展[J].中国药事,2005,19(7):445-447.
[4] 朱华,黄学萃,杨海广等.石斛属多糖的研究进展[J].医药导报,2007,26(12):1476-1479.
[5] CHEN S L,XIAO P G.Introduction to the sustainable utilization of Chinese herbal medicine resources[M].Beijing:China Medical Science and Technology Press,2006.
[6] 唐树梅,漆智平,陈雪华,等.营养胁迫对石斛兰植株形态和叶表面微结构的影响[J].电子显微学报,1999,18(5):503-506.
[7] 周俊.石斛的历史、分布与功能[J].中国药学杂志,2013,48(19):1609.
[8] 林萍,毕志明,徐红,等.石斛属植物药理活性研究进展[J].中草药,2003,34(11):附19-附21.
[9] MIYAZAWA M,SHIMAMURA H,NAKAMURA S,et a1.Antimutagenic activity of gigantol from Dendrobium nobile[J].J Agric Food Chem,1997,45(8):2849-2853.
[10] ONE M,ITO Y,MASUOKA C,et al.Antioxidative constituents from Dendrobii herba(Stams of Dendrobium spp.)[J].Food Sci Technol,1995,1(2):115-120.
[11] 钱桂敏,章华泼.金钗石斛化学成分及药理作用研究进展[J].中国中医药现代远程教育,2011,9(4):194-196.
[12] 王家玉.金钗的栽培[J].生物学通报,1994,29(6):42-43.
[13] 郑建铃.金钗石斛种苗快繁及林下栽培技术的初步研究[J].福建林业,2015(3):36-38,45.
[14] 徐红,王峥涛,丁家宜,等.药用石斛生物技术的研究概况[J].中国野生植物资源,2001,20(1):1-4.
[15] 吴辉,赵俊,宋锡全.四种不同培养基对诱导金钗石斛幼苗生根的影响[J].贵州科学,2007,25(2):65-67.
[16] 张明,夏鸿西,朱利泉,等.石斛组织培养研究进展[J].中国中药杂志,2000,25(6):323-326.
[17] 彭文书,马莉,赵苗苗,等.铁皮石斛组培苗生根条件优化研究[J].热带农业科学,2015,35(2):13-18.
[18] 索海翠,王科翰,李武,等.铁皮石斛组培苗生长差异与移栽成活率相关性研究[J].广东农业科学,2014(14):20-23.
[19] 熊庆娥.植物生理学实验教程[M].成都:四川科学技术出版社,2003:30-56.
[20] 刘石泉,董萌,赵运林.香蕉对疏花石斛试管苗生长的影响[J].中国林副特产,2009(6):10-12.
[21] 黎建玲,黄肇宇,詹源庆,等.金钗石斛试管苗生根研究[J].广西科学院学报,2006,22(2):87-89.
[22] 魏凤娟.铁皮石斛组织培养与栽培技术研究进展[J].广东农业科学,2010,37(4):81-85.
[23] 姚春,李泽生,李薇莎,等.串珠石斛组织培养与快速繁殖技术研究[J].安徽农业科学,2015,43(30):33-34,69.
[24] 林秀莲,马骁勇.‘阿里山’蝴蝶兰组织培养及其驯化移栽技术[J].亚热带农业研究,2015,11(1):31-34.
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