解淀粉芽孢杆菌HAB-9的鉴定及对17株植物病原菌的拮抗作用
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摘要
HAB-9是从槟榔树根际土壤中分离到的一株解淀粉芽孢杆菌(Bacillus amyloliquefaciens),该菌株对17株植物病原真菌有较好的拮抗作用。经16srDNA及生化特征测定,鉴定HAB-9菌株,应用对峙法测定了抑菌效果。结果显示,经16S rDNA鉴定,HAB-9菌株为B.amyloliquefacien;经生化特征测定,其革兰氏染色为阳性,M. R测定呈阴性,V-P测定为阳性,氧化酶测定为阴性,接触酶测定为阳性,淀粉水解试验发现具有解淀粉的功能;PDA平板对峙培养,发现HAB-9菌株对槟榔炭疽菌(Colletotrichum gloeosporioides)等17株植物病原真菌的抑菌活性有较大差异,平均抑制率为60.82%;对槟榔炭疽菌(C.gloeosporioides)有较好的拮抗作用,其抑菌率为70.59%;效果最差的为黄瓜炭疽病菌(C.orbiculare),抑菌率为50.22%。从本实验结果可知,HAB-9菌株对多数病原炭疽菌有较强的抑制作用,具有潜在的应用价值。
HAB-9 is a strain of Bacillus amyloliquefaciens isolated from rhizosphere soil of arecanut. The strain had good antagonistic effect on 17 strains of plant pathogenic fungi. HAB-9 strain was identified by using 16 S rDNA and biochemical characteristics. The antifungal effect was then determined by using the confrontation method. The results showed that strain HAB-9 was identified as B. amyloliquefacien by16 SrDNA. It is Gram positive, MR negative, V-P positive, oxidase negative and catalase positive. Starch hydrolysis test showed that it had the function of starch hydrolysis. PDA plate confrontation culture test found that the strain HAB-9 had a significant difference in an antifungal activity against 17 strains of plant pathogenic fungi like Collectotrichum gloeosporioides, with an average inhibition rate of 60.82%. It had a good antagonistic effect on Colletotrichum gloeosporioides, and its fungal inhibition rate was 70.59%. It had the worst inhibition effect on C.orbiculare, with an inhibition rate of 50.22%. According to the results of this experiment, HAB-9 strain has a high inhibitory effect on most of Colletotrichum pathogens, which indicates that it has a potential application value.
HAB-9 is a strain of Bacillus amyloliquefaciens isolated from rhizosphere soil of arecanut. The strain had good antagonistic effect on 17 strains of plant pathogenic fungi. HAB-9 strain was identified by using 16 S rDNA and biochemical characteristics. The antifungal effect was then determined by using the confrontation method. The results showed that strain HAB-9 was identified as B. amyloliquefacien by16 SrDNA. It is Gram positive, MR negative, V-P positive, oxidase negative and catalase positive. Starch hydrolysis test showed that it had the function of starch hydrolysis. PDA plate confrontation culture test found that the strain HAB-9 had a significant difference in an antifungal activity against 17 strains of plant pathogenic fungi like Collectotrichum gloeosporioides, with an average inhibition rate of 60.82%. It had a good antagonistic effect on Colletotrichum gloeosporioides, and its fungal inhibition rate was 70.59%. It had the worst inhibition effect on C.orbiculare, with an inhibition rate of 50.22%. According to the results of this experiment, HAB-9 strain has a high inhibitory effect on most of Colletotrichum pathogens, which indicates that it has a potential application value.
引文
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[6]卢娟，夏启玉，顾文亮，等.拮抗香蕉枯萎病菌的解淀粉芽孢杆菌LX1菌株的鉴定及其抗菌蛋白基因的克隆[J].热带作物学报，2013,34(1):117-124.
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[9]Marcus S, Ajay S, Owen P W. Developments in the use of Bacillus Species for Industrial Production[J]. Canadian Journal of Microbiology,2004,50(1):1-17.
[10]东秀珠，蔡妙英.常见细菌系统鉴定手册[M].北京：科学出版社，2001:370-398.
[11]刘雅婷，陈建斌.微生物实验手册[M].北京：中国农业出版社，2018:35-40.
[12]闫霜，吴洪生，周晓冬，等.黄瓜枯萎病生物防治研究进展[J].山东农业科学，2011(1):86-92.
[13]孙燕霞，张瑞清，刘保友，等.白地霉和解淀粉芽孢杆菌对黄瓜枯萎病的防治效果[J].山东农业科学，2010(12):55-57.
[2]刘俊.解淀粉芽孢杆菌C06防治桃褐腐病菌的机制及其产生的γ-多聚谷氨酸在定殖中的作用[D].南京：南京农业大学，2010.
[3]林毅，陈金辉，黄志鹏.芽孢杆菌几丁质酶及其在植物病虫害生物防治中的应用[J].福建农业科技，1998(S1):34-35.
[4]来航线，杨保伟，邱学礼. 9株芽孢杆菌的初步分离鉴定与拮抗性试验[J].西北农林科技大学学报（自然科学版），2004,32(7):93-96.
[5]张学君，朱桂宁，缪卫国，等. 5株芽孢杆菌对棉花病害的防治效果及其对棉苗生长的影响[J].棉花学报，1994,6(1):61-64.
[6]卢娟，夏启玉，顾文亮，等.拮抗香蕉枯萎病菌的解淀粉芽孢杆菌LX1菌株的鉴定及其抗菌蛋白基因的克隆[J].热带作物学报，2013,34(1):117-124.
[7]Priest F G,Goodfellow M,Shute L A,et al. Bacillus amyloliquefaciens sp. nov. nom. rev[J].International Journal of Systematic Bacteriology,1987,37(1):69-71.
[8]关晓欢，姜华.解淀粉芽孢杆菌研究进展[J].生物技术世界，2013(1):4,9.
[9]Marcus S, Ajay S, Owen P W. Developments in the use of Bacillus Species for Industrial Production[J]. Canadian Journal of Microbiology,2004,50(1):1-17.
[10]东秀珠，蔡妙英.常见细菌系统鉴定手册[M].北京：科学出版社，2001:370-398.
[11]刘雅婷，陈建斌.微生物实验手册[M].北京：中国农业出版社，2018:35-40.
[12]闫霜，吴洪生，周晓冬，等.黄瓜枯萎病生物防治研究进展[J].山东农业科学，2011(1):86-92.
[13]孙燕霞，张瑞清，刘保友，等.白地霉和解淀粉芽孢杆菌对黄瓜枯萎病的防治效果[J].山东农业科学，2010(12):55-57.
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