基于~1H-NMR的定坤丹指纹图谱研究
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摘要
目的建立定坤丹的~1H NMR指纹图谱,为其质量控制提供比较全面快速的评价方法。方法采用600 MHz NMR仪测定,建立10批次定坤丹甲醇水相和氯仿相的~1H NMR指纹图谱,利用SPSS统计分析软件计算批次间Pearson相关系数。结果建立了10批定坤丹样品的指纹图谱,可检测到糖类、有机酸类及芳香类化合物,10批供试品的指纹图谱间的相似系数均大于0. 9,与生成的对照图谱数据间相似系数均大于0. 95(P <0. 01),即呈现显著相关性,表明该复方总体质量较为稳定。结论该指纹图谱方法科学、准确且简便,可以为定坤丹的内在质量控制提供科学依据。
Objective To establish the ~1H NMR fingerprint of Dingkun Dan( DKD) and conduct a comprehensive and efficient method of quality evaluation. Methods The 600 MHz ~1H-NMR was employed to establish the ~1H NMR fingerprint of 10 batches of DKD.DKD samples were extracted with chloroform-methanol-aqueous. The Pearson correlation coefficient between batches was calculated using SPSS statistical analysis software. Results The ~1H NMR fingerprint of 10 batches of DKD were established. Carbohydrate,organic acids,aromatic compounds and other compounds were detected. The Pearson correlation coefficient values of the DKD were all above 0. 9,and the coefficient compared with generated control group data were all above 0. 95( P < 0. 01),which showed significant correlation,indicating a relatively stable quality of the formula. Conclusion The ~1H-NMR analysis method established in this study is scientific,accurate,reliable,and simple,and it can also supply a solid scientific basis for the further quality control of DKD.
Objective To establish the ~1H NMR fingerprint of Dingkun Dan( DKD) and conduct a comprehensive and efficient method of quality evaluation. Methods The 600 MHz ~1H-NMR was employed to establish the ~1H NMR fingerprint of 10 batches of DKD.DKD samples were extracted with chloroform-methanol-aqueous. The Pearson correlation coefficient between batches was calculated using SPSS statistical analysis software. Results The ~1H NMR fingerprint of 10 batches of DKD were established. Carbohydrate,organic acids,aromatic compounds and other compounds were detected. The Pearson correlation coefficient values of the DKD were all above 0. 9,and the coefficient compared with generated control group data were all above 0. 95( P < 0. 01),which showed significant correlation,indicating a relatively stable quality of the formula. Conclusion The ~1H-NMR analysis method established in this study is scientific,accurate,reliable,and simple,and it can also supply a solid scientific basis for the further quality control of DKD.
引文
[1]中华人民共和国药典编委会.中国药典·一部[M].北京:中国医药科技出版社,2015:1120-1121.
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[12]刘东方,赵丽娜,李银峰,等.中药指纹图谱技术的研究进展及应用[J].中草药,2016,47(22):4085-4094.
[13]梁悦.生脉注射液物理指纹图谱的建立及其质量评价[J].中草药,2018,49(11):2571-2576.
[2]赵卫萍,范树军,高云秀.定坤丹治疗青春期原发性痛经30例[J].中医杂志,2005,46(8):609-610.
[3]王燕.定坤丹治疗原发性痛经300例[J].陕西中医,2010,31(3):278-280.
[4]马堃,陈燕霞,王艳英.定坤丹对寒凝血瘀型痛经患者血清GPF2α,ET,PAF及子宫动脉血流动力学的影响[J].中国中药杂志,2017,42(23):4474-4480.
[5]易星星,林洁.定坤丹治疗肾虚肝郁证月经过少患者30例临床观察[J].中国中西医结合杂志,2016,36(5):629-631.
[6]江敏.补肾活血汤联合定坤丹序贯治疗育龄期肾虚血瘀型PCOS的临床[D].武汉:湖北中医药大学,2017.
[7]王玉雯.定坤丹治疗妇科疾病应用体会[J].中医药信息杂志,2007,14(9):78.
[8]张西芝.定坤丹为主治疗子宫内膜异位症37例[J].中医杂志,2005,46(4):286.
[9]李静,魏玉海,秦雪梅,等.基于NMR代谢组学技术的当归不同部位化学成分比较[J].中草药,2017,48(7):1409-1415.
[10]马晓丽,邹萍萍,雷伟,等.定量核磁技术参数的优化及其在中草药定量分析领域的应用[J].药学学报,2014,49(9):1248-1257.
[11]曲婷丽,魏玉海,李爱平,等.黄芪注射液1H-NMR指纹图谱研究[J].药学学报,2016,51(5):780-785.
[12]刘东方,赵丽娜,李银峰,等.中药指纹图谱技术的研究进展及应用[J].中草药,2016,47(22):4085-4094.
[13]梁悦.生脉注射液物理指纹图谱的建立及其质量评价[J].中草药,2018,49(11):2571-2576.